- Volume 15 Issue 4
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Expression of Transcription Factor FOXC2 in Cervical Cancer and Effects of Silencing on Cervical Cancer Cell Proliferation
- Zheng, Chun-Hua (Gynecology, The First Hospital of Jilin University) ;
- Quan, Yuan (Gynecology, The First Hospital of Jilin University) ;
- Li, Yi-Yang (Gynecology, The First Hospital of Jilin University) ;
- Deng, Wei-Guo (Public Health, Jilin University) ;
- Shao, Wen-Jing (Gynecology, The First Hospital of Jilin University) ;
- Fu, Yan (Gynecology, The First Hospital of Jilin University)
- Published : 2014.02.28
Objective: Forkhead box C2 (FOXC2) is a member of the winged helix/forkhead box (Fox) family of transcription factors. It has been suggested to regulate tumor vasculature, growth, invasion and metastasis, although it has not been studied in cervical cancer. Here, we analyzed FOXC2 expression in cervical tissues corresponding to different stages of cervical cancer development and examined its correlation with clinicopathological characteristics. In addition, we examined the effects of targeting FOXC2 on the biological behavior of human cervical cancer cells. Methods: The expression of FOXC2 in normal human cervix, CIN I-III and cervical cancer was examined by immunohistochemistry and compared among the three groups and between cervical cancers with different pathological subtypes. Endogenous expression of FOXC2 was transiently knocked down in human Hela and SiHa cervical cells by siRNA, and cell viability and migration were examined by scratch and CCK8 assays, respectively. Results: In normal cervical tissue the frequency of positive staining was 25% (10/40 cases), with a staining intensity (PI) of
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