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Inhibitory Effects of α-Pinene on Hepatoma Carcinoma Cell Proliferation

  • Chen, Wei-Qiang (School of Basic Medicine and Guangdong Key Laboratory for Bioactive Drug Research, Guangdong Pharmaceutical University) ;
  • Xu, Bin (School of Life Science and Biopharmaceutical Sciences, Guangdong Pharmaceutical University) ;
  • Mao, Jian-Wen (School of Basic Medicine and Guangdong Key Laboratory for Bioactive Drug Research, Guangdong Pharmaceutical University) ;
  • Wei, Feng-Xiang (The Genetics Laboratory, Maternity and Child Healthcare Hospital, Longgang District) ;
  • Li, Ming (School of Basic Medicine and Guangdong Key Laboratory for Bioactive Drug Research, Guangdong Pharmaceutical University) ;
  • Liu, Tao (School of Basic Medicine and Guangdong Key Laboratory for Bioactive Drug Research, Guangdong Pharmaceutical University) ;
  • Jin, Xiao-Bao (School of Basic Medicine and Guangdong Key Laboratory for Bioactive Drug Research, Guangdong Pharmaceutical University) ;
  • Zhang, Li-Rong (School of Basic Medicine and Guangdong Key Laboratory for Bioactive Drug Research, Guangdong Pharmaceutical University)
  • Published : 2014.04.01

Abstract

Background: Pine needle oil from crude extract of pine needles has anti-tumor effects, but the effective component is not known. Methods: In the present study, compounds from a steam distillation extract of pine needles were isolated and characterized. Alpha-pinene was identified as an active anti-proliferative compound on hepatoma carcinoma BEL-7402 cells using the MTT assay. Results: Further experiments showed that ${\alpha}$-pinene inhibited BEL-7402 cells by arresting cell growth in the G2/M phase of the cell cycle, downregulating Cdc25C mRNA and protein expression, and reducing cycle dependence on kinase 1(CDK1) activity. Conclusion: Taken together, these findings indicate that ${\alpha}$-pinene may be useful as a potential anti-tumor drug.

Keywords

${\alpha}$-pinene;hepatoma carcinoma;proliferation;cell cycle proteins

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