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BMI1 and TWIST1 Downregulated mRNA Expression in Basal Cell Carcinoma

  • Rajabpour, Fatemeh Vand (Cellular and Molecular Research Center, Shahrekord University of Medical Sciences) ;
  • Raoofian, Reza (Medical Genetics Department, Tehran University of Medical Sciences) ;
  • Youssefian, Leila (Medical Genetics Department, Tehran University of Medical Sciences) ;
  • Vahidnezhad, Hassan (Medical Genetics Department, Tehran University of Medical Sciences) ;
  • Shahshahani, Mostafa Mirshams (Tumor Clinic, Pathology Department and the Department of Plastic and Reconstructive Surgery, Razi Dermatology Hospital, Tehran University of Medical Sciences) ;
  • Fathi, Hamidreza (Tumor Clinic, Pathology Department and the Department of Plastic and Reconstructive Surgery, Razi Dermatology Hospital, Tehran University of Medical Sciences) ;
  • Noormohammadpour, Pedram (Tumor Clinic, Pathology Department and the Department of Plastic and Reconstructive Surgery, Razi Dermatology Hospital, Tehran University of Medical Sciences) ;
  • Hesari, Kambiz Kamyab (Tumor Clinic, Pathology Department and the Department of Plastic and Reconstructive Surgery, Razi Dermatology Hospital, Tehran University of Medical Sciences) ;
  • Hashemzadeh-Chaleshtori, Morteza (Cellular and Molecular Research Center, Shahrekord University of Medical Sciences) ;
  • Tabrizi, Mina (Medical Genetics Department, Tehran University of Medical Sciences)
  • Published : 2014.04.30

Abstract

Background: BMI1, TWIST1 and SNAI2/SLUG have been implicated in aggressive behavior of squamous cell carcinoma (SCC) and melanoma and BMI1 expression could identify subtypes of Merkel cell carcinoma (MCC). However, BMI1, TWIST1 and SNAI2 expression levels in basal cell carcinomas (BCCs) have not been elucidated. We hypothesized BCC could be a good model system to decipher mechanisms which inhibit processes that drive tumor metastasis. The aim of this study was to examine the mRNA expression level of BMI1, TWIST1, and SNAI2 in BCCs. Materials and Methods: Thirty-five fresh non-metastatic BCC tissue samples and seven fresh normal skin tissue samples were evaluated by real-time RT-PCR. Results: BMI1 and TWIST1 demonstrated marked down-regulation (p<0.00l, p=0.00l respectively), but SNAI2 showed no significant change (p=0.12). Conclusions: Previous literature has clearly demonstrated a positive association between BMI1 and TWIST1 expression and metastatic BCC, aggressive SCC and melanoma. Here, we demonstrated a negative association between BMI1 and TWIST1 mRNA expression level and BCC.

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