Analysis of Different Activation Statuses of Human Mammary Epithelial Cells from Young and Old Groups

  • Feng, Chen-Chen (College of Bioinformatics Science and Technology, Harbin Medical University) ;
  • Chen, Li-Na (College of Bioinformatics Science and Technology, Harbin Medical University) ;
  • Chen, Mei-Jun (College of Bioinformatics Science and Technology, Harbin Medical University) ;
  • Li, Wan (College of Bioinformatics Science and Technology, Harbin Medical University) ;
  • Jia, Xu (College of Bioinformatics Science and Technology, Harbin Medical University) ;
  • Zhou, Yan-Yan (College of Bioinformatics Science and Technology, Harbin Medical University) ;
  • He, Wei-Ming (Institute of Opto-electronics, Harbin Institute of Technology)
  • Published : 2014.04.30


Human mammary epithelial cells have different proliferative statuses and demonstrate a close relationship with age and cell proliferation. Research on this topic could help understand the occurrence, progression and prognosis of breast cancer. In this article, using significance analysis of a microarray algorithm, we analyzed gene expression profiles of human mammary epithelial cells of different proliferative statuses and different age groups. The results showed there were significant differences in gene expression in the same proliferation status between elderly and young groups. Three common differentially expressed genes were found to dynamically change with the proliferation status and to be closely related to tumorigenesis. We also found elderly group had less status-related differential genes from actively proliferating status to intermediate status and more statusrelated differential genes from intermediate status than the young group. Finally, functional enrichment analyses allowed evaluation of the detailed roles of these differentially-expressed genes in tumor progression.


  1. Upadhyay AK, Ajay AK, Singh S, Bhat MK (2008) Cell cycle regulatory protein 5 (Cdk5) is a novel downstream target of ERK in carboplatin induced death of breast cancer cells. Curr Cancer Drug Targets, 8, 741-52.
  2. Romanov SR, Kozakiewicz BK, Holst CR, et al (2001). Normal human mammary epithelial cells spontaneously escape senescence and acquire genomic changes. Nature, 409, 633-7.
  3. Tam KW, Ho CT, Lee WJ, et al (2013). Alteration of alphatocopherol-associated protein (TAP) expression in human breast epithelial cells during breast cancer development. Food Chem, 138, 1015-21.
  4. Tusher VG, Tibshirani R, Chu G (2001). Significance analysis of microarrays applied to the ionizing radiation response. Proc Natl Acad Sci U S A, 98, 5116-21.
  5. Xing Z, Zhang Y, Zhang X, et al (2013). Fangchinoline Induces G1 Arrest in Breast Cancer Cells Through Cell-Cycle Regulation. Phytother Res, 27, 1790-4
  6. Yang M, Yuan F, Li P, et al (2012). Interferon regulatory factor 4 binding protein is a novel p53 target gene and suppresses cisplatin-induced apoptosis of breast cancer cells. Mol Cancer, 11, 54.
  7. Yau C, Fedele V, Roydasgupta R, et al (2007). Aging impacts transcriptomes but not genomes of hormone-dependent breast cancers. Breast Cancer Res, 9, 59.
  8. Yu M, Bardia A, Wittner BS, et al (2013). Circulating breast tumor cells exhibit dynamic changes in epithelial and mesenchymal composition. Science, 339, 580-4.
  9. Yuan Y, Nymoen DA, Dong HP, et al (2009). Expression of the folate receptor genes FOLR1 and FOLR3 differentiates ovarian carcinoma from breast carcinoma and malignant mesothelioma in serous effusions. Hum Pathol, 40, 1453-60.
  10. Huang da W, Sherman BT, Lempicki RA (2009). Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources. Nat Protoc, 4, 44-57.
  11. Didier ES, Sugimoto C, Bowers LC, et al (2012). Immune correlates of aging in outdoor-housed captive rhesus macaques (Macaca mulatta). Immun Ageing, 9, 25.
  12. Du H, Huang X, Wang S, et al (2009). PSMA7, a potential biomarker of diseases. Protein Pept Lett, 16, 486-9.
  13. Eivazi-Ziaei J, Dastgiri S, Kermani IA, et al (2011). Age pattern of the occurrence of breast cancer in the northwest of Iran. Indian J Cancer, 48, 406-9.
  14. Iwanaga Y, Chi YH, Miyazato A, et al (2007). Heterozygous deletion of mitotic arrest-deficient protein 1 (MAD1) increases the incidence of tumors in mice. Cancer Res, 67, 160-6.
  15. Jemal A, Siegel R, Ward E, et al (2009). Cancer statistics, 2009. CA Cancer J Clin, 59, 225-49.
  16. Patel JB, Patel KD, Patel SR, et al (2012). Recent candidate molecular markers: vitamin D signaling and apoptosis specific regulator of p53 (ASPP) in breast cancer. Asian Pac J Cancer Prev, 13, 1727-35.

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