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Stathmin is a Marker of Progression and Poor Prognosis in Esophageal Carcinoma

  • Wang, Feng (Department of Oncology, the First Affiliated Hospital of Zhengzhou University) ;
  • Xuan, Xiao-Yan (Department of Microbiology and Immunology, Zhengzhou University) ;
  • Yang, Xuan (Department of Microbiology and Immunology, Zhengzhou University) ;
  • Cao, Lei (Department of Oncology, the First Affiliated Hospital of Zhengzhou University) ;
  • Pang, Li-Na (Department of Oncology, the First Affiliated Hospital of Zhengzhou University) ;
  • Zhou, Ran (Department of Oncology, the First Affiliated Hospital of Zhengzhou University) ;
  • Fan, Qin-Xia (Department of Oncology, the First Affiliated Hospital of Zhengzhou University) ;
  • Wang, Liu-Xing (Department of Oncology, the First Affiliated Hospital of Zhengzhou University)
  • Published : 2014.04.30

Abstract

Stathmin, also called oncoprotein 18, is a founding member of the family of microtubule-destabilizing proteins that play a critical role in the regulation of mitosis. At the same time stathmin has been recognized as one of responsible factors in cancer cells. The aim of this study was to assess stathmin status, its correlations with clinicopathological parameters and its role as a progosnostic marker in EC patients. The protein and mRNA levels of stathmin were examined byimmunohistochemistry (IHC) and in situ hybridization in 100EC tissues and adjacent noncancerous tissues. mRNA and protein expression of stathmin in three EC cell lines(EC9706, ECa109, EC1 commonly used in research) were also analyzed using immunocytochemistry, western blot and in situ hybridization. The prognostic value of Stathmin expression within the tumor tissues were assessed by Cox regression and Kaplan-Meier analysis. We showed that stathmin expression was significantly higher in EC tissues than in adjacent noncancerous tissues. High stathmin immunostaining score in the EC was positively correlated with tumor differentiation, Tumor invasion, Lymph node metastases, and TNM stage. In addition, we demonstrated that three EC cell lines examined, were constitutively expressing a high level of stathmin. Of those, EC-1 showed the strongest mRNA and protein expression for the stathmin analyzed. Kaplan-Meier analysis showed that significantly longer 5-year survival rate was seen in EC patients with high Stathmin expression, compared to those with low expression of Stathmin expression. Furthermore, multivariate Cox proportional hazard analyses revealed that Stathmin was an independent factors affecting the overall survival probability. In conclusion, our data provide a basis for the concept that stathmin might be associated with EC development and progression. High levels of Stathmin expression in the tumor tissues may be a good prognostic marker for patients with EC.

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