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Effects of Tissue Factor, PAR-2 and MMP-9 Expression on Human Breast Cancer Cell Line MCF-7 Invasion

  • Lin, Zeng-Mao (Breast Disease Center, Peking University First Hospital) ;
  • Zhao, Jian-Xin (Breast Disease Center, Peking University First Hospital) ;
  • Duan, Xue-Ning (Breast Disease Center, Peking University First Hospital) ;
  • Zhang, Lan-Bo (Breast Disease Center, Peking University First Hospital) ;
  • Ye, Jing-Ming (Breast Disease Center, Peking University First Hospital) ;
  • Xu, Ling (Breast Disease Center, Peking University First Hospital) ;
  • Liu, Yin-Hua (Breast Disease Center, Peking University First Hospital)
  • Published : 2014.01.30

Abstract

Objective: This study aimed to explore the expression of tissue factor (TF), protease activated receptor-2 (PAR-2), and matrix metalloproteinase-9 (MMP-9) in the MCF-7 breast cancer cell line and influence on invasiveness. Methods: Stable MCF-7 cells transfected with TF cDNA and with TF ShRNA were established. TF, PAR-2, and MMP-9 protein expression was analyzed using indirect immunofluorescence and invasiveness was evaluated using a cell invasion test. Effects of an exogenous PAR-2 agonist were also examined. Results: TF protein expression significantly differed between the TF cDNA and TF ShRNA groups. MMP-9 protein expression was significantly correlated with TF protein expression, but PAR-2 protein expression was unaffected. The PAR-2 agonist significantly enhanced MMP-9 expression and slightly increased TF and PAR-2 expression in the TF ShRNA group, but did not significantly affect protein expression in MCF-7 cells transfected with TF cDNA. TF and MMP-9 expression was positively correlated with the invasiveness of tumor cells. Conclusion: TF, PAR-2, and MMP-9 affect invasiveness of MCF-7 cells. TF may increase MMP-9 expression by activating PAR-2.

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