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Comparison of Gangjihwan and Combination of Gangjihwan and Gamisochehwan in the Improvement Effects of Nonalcoholic Fatty Liver Disease in a High Fat Diet-Fed NAFLD Mouse Model

고지방식이 비만마우스 모델에서 강지환(降脂丸)과 강지환(降脂丸)+가미소체환(加味消滯丸)의 비알콜성 지방간질환 개선효과 비교

  • Received : 2014.05.15
  • Accepted : 2014.06.16
  • Published : 2014.06.30

Abstract

Objectives : This study investigated the improvement effects of Gangjihwan (DF) and combination of Gangjihwan and Gamisochehwan (GSH) on nonalcoholic fatty liver disease in a high fat diet-induced obese mouse model. Methods : Eight-week-old C57BL/6N mice were divided into five groups: a normal lean group given a standard diet, an obese control group given a high fat diet, and atorvastatin, DF, and DF+GSH groups given a high fat diet with atorvastatin (10 mg/kg), DF (40 mg/kg), and DF+GSH (80 mg/kg), respectively. After 8 weeks of treatment, body weight gain, blood lipid markers, ALT concentrations, liver weight and histology were examined. Results : 1. Body weight gain was significantly decreased in DF, DF+GSH and atorvastatin groups compared with control. The extent of decreases was eminent in DF+GSH group. 2. Circulating concentrations of total cholesterol, HDL-cholesterol and LDL-cholesterol were decreased in DF, DF+GSH and atorvastatin groups compared with control. The decreases were significant in DF+GSH and atorvastatin groups. 3. Liver weights were decreased in DF, DF+GSH and atorvastatin groups compared with control. In particular, liver weight was significantly reduced only in DF+GSH group. 4. Hepatic lipid accumulation was significantly decreased in DF, DF+GSH and atorvastatin groups compared with control, and the magnitude of which was more effective in DF+GSH group than in DF-only group. 5. Circulating ALT concentrations were decreased in DF, DF+GSH and atorvastatin compared with control, but ALS levels were significantly reduced only in DF+GSH group. Conclusions : In conclusion, these results suggest that DF decreases body weight gain, improves blood lipid metabolism, and reduces liver weight and hepatic lipid accumulation, contributing to the improvement of nonalcoholic fatty liver disease. In addition, these effects were more effective in DF+GSH combination group than in DF-only group.

Keywords

Gangjihwan;nonalcoholic fatty liver disease;Gamisochehwan;ALT;cholesterol

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Cited by

  1. Comparison of Gangjihwan-1,2,3,4 and Combination of Gangjihwan-1 and Gamisochehwan in the Improvement Effects of Nonalcoholic Fatty Liver Disease in a High Fat Diet-Fed Obese Mouse Model vol.29, pp.6, 2014, https://doi.org/10.6116/kjh.2014.29.6.149.
  2. Comparison of Gangjihwan and Combination of Gangjihwan and Gamisochehwan in the Improvement Effects of Weight Loss in a High Fat Diet-Fed Obese Mice vol.22, pp.2, 2014, https://doi.org/10.14374/HFS.2014.22.2.105
  3. Comparison of Gangji-hwan-1, 2, 3, 4 and Combination of Gangji-hwan-1 and Gamisoche-hwan in the Reducing Effects of Body Weight in a High Fat Diet-Fed Obese Mice vol.15, pp.01, 2015, https://doi.org/10.15429/jkomor.2015.15.1.9

Acknowledgement

Supported by : National Research Foundation of Korea(NRF)