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The Electrophysiology Application on Guard Cells to See the Influence of Carbon Dioxide

  • Lee, Joon-Sang
  • Received : 2014.02.10
  • Accepted : 2014.04.15
  • Published : 2014.05.30

Abstract

The effect of $CO_2$ on the opening of stomata in the intact leaf of Commelina communis has been investigated. Full opening of stomatal apertures(around $18{\mu}m$) was achieved in the intact leaf by addition of $CO_2$($900{\mu}mol\;mol^{-1}$). At 90 minutes, the stomatal apertures of leaves treated with $CO_2$ free air were reduced. In contrast, stomata opened most widely with the treatment of $CO_2$ air at 90 minutes. The effects of light, $CO_2$ air and $CO_2$ free air on the change of membrane potential difference(PD) were measured. Fast hyperpolarization of guard cell membrane PD was recorded reaching up to -12 mV in response to light. If $CO_2$ free air was given firstly, there was no response. When light was given after $CO_2$ free air, the light effect was very clear. At the onset of $CO_2$ air, the PD showed a dramatic hyperpolarization to about -25 mV. Changes in the pH of apoplast in intact leaves in response to $CO_2$ air were observed. $CO_2$ air caused a change of 0.4 pH unit. Therefore, it can be hypothesized that $CO_2$ flowing could stimulate proton efflux which is a necessary precursor of stomatal opening.

Keywords

$CO_2$;Commelina communis;Stomatal aperture;Membrane potential difference

References

  1. Pallaghy, C. K., 1968, Electrophysiological studies in guard cells of tobacco. Plant Physiol., 64, 79-82.
  2. Ramos, C., Hall, A. E., 1983, Effects of photofluorescence rate and intercellular partial pressure on leaf conductance and $CO_2$ uptake rate in Capsicum and Amaranthus, Photosynthetica, 17, 34-42.
  3. Raschke, K., 1976. How stomata resolve the dilemma of opposing priorities. Philosophical Transactions of the Royal Society, ser, B, 273, 551-560. https://doi.org/10.1098/rstb.1976.0031
  4. Raschke, K., Humble, G. D., 1973, No uptake of ions required by opening stomata of Vicia faba, Planta, 115, 47-57. https://doi.org/10.1007/BF00388604
  5. Shaish, A., Roth-Bejerano, N., Itai, C., 1989, The response of stomata to relate to its effect on respiration and ATP level, Physiol. Plantarum, 76, 107-111. https://doi.org/10.1111/j.1399-3054.1989.tb05460.x
  6. Taiz, L., Zeiger, E., 2007, Plant Physiology. 4th ed, Sunderland: Sinauet Associates.
  7. Weyers, J. D. B., Travis, A. J., 1981, Selection and preparation of leaf epidermis for experiments on stomatal physiology, J Exp. Bot., 32, 837-850. https://doi.org/10.1093/jxb/32.4.837
  8. Wong, S. C., Cowan, I. R., Farquhar, G. D., 1978, Leaf conductance in relation to assimilation in Eucalyptus pauciflora Sieb. ex Spreng, Plant Physiol., 62, 670-674. https://doi.org/10.1104/pp.62.4.670
  9. Zeiger, E., 1986, The photobiology of stomatal movements. in: Kendrick, R. E. and Krononberg, G. H. M., (eds,), Photomorphogenesis in plants, Martinus Nijhorff Publishers, Dordrecht, Netherlands, 391-413.
  10. Zeiger, E., 1983, The biology of stomatal guard cells. Ann. Rev. Plant Physiol., 34, 441-475. https://doi.org/10.1146/annurev.pp.34.060183.002301
  11. Melis, A., Zeiger, E., 1982, Modulation of guard cell photophsphorylation by $CO_2$, Plant Physiol., 69, 642-647. https://doi.org/10.1104/pp.69.3.642
  12. Lee, J. S., Bowling, D. J. F., 1992, Effect of the mesophyll on stomatal opening in Commelina communis, J Exp. Bot., 43, 951-957. https://doi.org/10.1093/jxb/43.7.951
  13. Lee, J. S., Bowling, D. J. F., 1993, Influence of the mesophyll on the change of electrical potential difference of guard cells induced by red light and $CO_2$ in Commelina communis L. and Tradescantina virginiana L, Kor. J. Bio., 36, 383-389.
  14. Leymarie, J., Vavasseur, A., Lsaceve, G., 1999, $CO_2$ sensingin stomata of abi1-1 and abi2-1 mutants of Arabidopsis thaliana, Plant Physiol. Biochem, 36, 539-543.
  15. Meidner, H., Mansfield, T. A., 1965, Stomatal response to illumination, Biological Review, 40, 483-509. https://doi.org/10.1111/j.1469-185X.1965.tb00813.x
  16. Morison, J. I. L., 1987, Intercellular concentration and stomatal response to $CO_2$, in: Zeiger, E., Farquhar, G.D., Cowan, I. R., (eds.), Stomatal function, Stanford, California, USA, 229-251.
  17. Ogawa, T., Ishikawa, K., Shimada, K., Shibata, K., 1978, Synergistic action of red and blue light and action spectra for malate formation in guard cells of Vicia faba L., Planta, 142, 61-65. https://doi.org/10.1007/BF00385121
  18. Lee, J. S., Bowling, D. J. F., 1995, Influence of the mesophyll on stomatal opening, Aust. J Plant Physiol., 22, 357-363. https://doi.org/10.1071/PP9950357
  19. Lee, J. S., 2010. Stomatal opening mechanism of CAM plants, J Plant Biol., 53, 19-23. https://doi.org/10.1007/s12374-010-9097-8
  20. Lee, J. S., 2013. Do really close stomata by soil drying ABA produced in the roots and transported in transpiration stream? American J Plant Science, 4: 169-173. https://doi.org/10.4236/ajps.2013.41022
  21. Higinbotham, N., 1973, Electropotentials of plant cells, Ann. Rev. Plant Physiol., 24, 25-46. https://doi.org/10.1146/annurev.pp.24.060173.000325
  22. Edwards, M. C., Smith, G. N., Bowing, D. J. F., 1988, Guard cells extrude protons prior to stomatal opening, J Exp. Bot., 39, 1541-1547. https://doi.org/10.1093/jxb/39.11.1541
  23. Farquhar, G. D., Dubbe, D. R., Raschke, K., 1978, Gain of the feedback loop involving carbon dioxide and stomata, Plant Physiol., 62, 406-412. https://doi.org/10.1104/pp.62.3.406
  24. Gotow, K., Taylor, S., Zeiger, E., 1988, Photosynthetic carbon fixation in guard cell protoplasts of Vicia faba L, Plant Physiol., 86, 700-705. https://doi.org/10.1104/pp.86.3.700
  25. Hsiao, T. C., Allaway, W. G., Evans, L. T., 1973, Action spectra for guard cell $Rb^{2+}$ uptake and stomatal opening in Vicia faba, Plant Physiol., 51, 82-88. https://doi.org/10.1104/pp.51.1.82
  26. Jarvis, P. G., Morison, J. I. L., 1981, The control of transpiration and photosynthesis by the stomata, in: P. G. Jarvis, and Mansfield, T. A. (eds,), Stomatal physiology, Cambridge University Press, 247-279.
  27. Kim, C. Y., 2012, Stomatal responses of C3 and C4 cyperus species(Cyperaceae) in Korea to elevated $CO_2$ concentration, M.S.D. Dessetation, Sungshin Women's University, Seoul, Korea.
  28. Kim, D. J., Lee, J. S., 2007, Current theories for mechanism of stomatal opening, J Plant Biol., 50, 523-526. https://doi.org/10.1007/BF03030704
  29. Kuiper, P. J. C., 1964, Dependence upon wavelength of stomatal movement in epidermal tissue of Senecio odoris, Plant Physiol., 39, 952-955. https://doi.org/10.1104/pp.39.6.952
  30. Bowing, D. J. F., 1989, Intracellular and intercellular pH measurement with microelectrodes, Methods Enzym., 174, 331-338. https://doi.org/10.1016/0076-6879(89)74026-X

Acknowledgement

Supported by : Chungbuk National University