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Storage of Bull and Boar Semen: Novel Concepts Derived Using Magnetized Water and Antioxidants

  • Lee, Sang-Hee (College of Animal Life Science, Kangwon National University) ;
  • Cheong, Hee-Tae (School of Veterinary Medicine, Kangwon National University) ;
  • Yang, Boo-Keun (College of Animal Life Science, Kangwon National University) ;
  • Park, Choon-Keun (College of Animal Life Science, Kangwon National University)
  • Received : 2014.03.03
  • Accepted : 2014.03.12
  • Published : 2014.03.31

Abstract

Artificial insemination technique has been contributed immensely for production of livestock worldwide as a critical assisted reproductive technique to preserve and propagate excellent genes in domestic animal industry. In the past decade, methods for semen preservation have been improved mostly in liquid preservation method for boar semen and freezing method for bull semen. Among many factors affecting semen quality during preservation, reactive oxygen species, produced by aerobic respiration in sperm for survival and motility, are unfavorable to sperm physiology. In mammalian cell as well as in the sperm, antioxidant system plays a role in degradation of reactive oxygen species. Magnetized water forms smaller stabilizing water clusters, resulting in high absorption and permeability of the cell for water, implicating its application for semen preservation. Therefore, this review focuses on preservation methods of boar and bull semen with respect to improvement of extender and reduction of reactive oxygen species by using magnetized water and supplementation of antioxidants.

Acknowledgement

Supported by : IPET

References

  1. Tigrek S, Barnes F (2010). Water structures and effects of electric and magnetic fields. In: Non-Thermal Effects and Mechanisms of Interaction between Electromagnetic Fields and Living Matter. Giuliani, Soffritti, Eds and Fidenza editors. Bologna Italy: An ICEMS Monograph, pp. 25-50.
  2. Watson P (2000): The causes of reduced fertility with cryopreserved semen. Anim Reprod Sci 60:481-492.
  3. White I (1993): Lipids and calcium uptake of sperm in relation to cold shock and preservation: a review. Reprod Fertil Dev 5(6):639-658. https://doi.org/10.1071/RD9930639
  4. Woelders H, Matthijs A, Engel B (1997): Effects of trehalose and sucrose, osmolality of the freezing medium, and cooling rate on viability and intactness of bull sperm after freezing and thawing. Cryobiology 35(2):93-105. https://doi.org/10.1006/cryo.1997.2028
  5. Woo MW, Mujumdar AS (2010): Effects of electric and magnetic field on freezing and possible relevance in freeze drying. Drying Technol 28(4):433-443. https://doi.org/10.1080/07373930903202077
  6. Zhou KX, Lu GW, Zhou QC, Song JH, Jiang ST, Xia HR (2000): Monte Carlo simulation of liquid water in a magnetic field. J Appl Phys 88(4):1802-1805. https://doi.org/10.1063/1.1305324
  7. Zhou Z, Zhao H, Han J (2012): Supercooling and crystallization of water under DC magnetic fields. CIESC J 63:1405-1408. (Received: 3 March 2014/ Accepted: 12 March 2014)
  8. Wang X, Sharma RK, Sikka SC, Thomas Jr AJ, Falcone T, Agarwal A (2003): Oxidative stress is associated with increased apoptosis leading to spermatozoa DNA damage in patients with male factor infertility. Fertil Steril 80(3):531-535. https://doi.org/10.1016/S0015-0282(03)00756-8
  9. Roca J, Vazquez JM, Gil MA, Cuello C, Parrilla I, Martinez EA (2006): Challenges in pig artificial insemination. Reprod Domest Anim 41(2):43-53.
  10. Rodríguez-Gil J, Rigau T (1995): Effects of slight agitation on the quality of refrigerated boar sperm. Anim Reprod Sci 39(2):141-146. https://doi.org/10.1016/0378-4320(95)01390-L
  11. Salisbury G, Lodge J (1963): Respiratory quotients of bovine spermatozoa. J Reprod Fertil 5(2):195-202. https://doi.org/10.1530/jrf.0.0050195
  12. Sanocka D, Kurpisz M (2004): Reactive oxygen species and sperm cells. Reprod Biol Endocrinol 2(12):1-7. https://doi.org/10.1186/1477-7827-2-1
  13. Shimatsu Y, Uchida M, Niki R, Imai H (2002): Liquid storage of miniature boar semen. Exp Anim 51(2):143-147. https://doi.org/10.1538/expanim.51.143
  14. Storey BT (1997): Biochemistry of the induction and prevention of lipoperoxidative damage in human spermatozoa. Molecular Hum Reprod 3(3): 203-213. https://doi.org/10.1093/molehr/3.3.203
  15. Stradaioli G, Noro T, Sylla L, Monaci M (2007): Decrease in glutathione (GSH) content in bovine sperm after cryopreservation: comparison between two extenders. Theriogenology 67(7):1249-1255. https://doi.org/10.1016/j.theriogenology.2007.01.009
  16. SZCZESNIAK-FABIANCZYK B, Bochenek M, SMORĄG Z, Ryszka F (2003): Effect of antioxidants added to boar semen extender on the semen survival time and sperm chromatin structure. Reprod Biol 3:81-87.
  17. Szkatula A, Balanda M, Kopeć M (2002): Magnetic treatment of industrial water. Silica activation. Eur Phys J Applied Physics 18(01):41-49. https://doi.org/10.1051/epjap:2002025
  18. Thiangtum K, Pinyopummin A, Hori T, Kawakami E, Tsutsui T (2009): Effect of catalase and superoxide dismutase on motility, viability and acrosomal integrity of frozen‐thawed cat spermatozoa. Reprod Domest Anim 44(s2):369-372. https://doi.org/10.1111/j.1439-0531.2009.01420.x
  19. Michael A, Alexopoulos C, Pontiki E, Hadjipavlou- Litina D, Saratsis P, Boscos C (2007): Effect of antioxidant supplementation on semen quality and reactive oxygen species of frozen-thawed canine spermatozoa. Theriogenology 68(2):204-212. https://doi.org/10.1016/j.theriogenology.2007.04.053
  20. O'Flaherty C, de Lamirande E, Gagnon C (2006): Reactive oxygen species modulate independent protein phosphorylation pathways during human sperm capacitation. Free Radicals Biol Med 40(6):1045-1055. https://doi.org/10.1016/j.freeradbiomed.2005.10.055
  21. Oehninger S, Blackmore P, Mahony M, Hodgen G (1995): Effects of hydrogen peroxide on human spermatozoa. J Assisted Reprod Genet 12(1):41-47. https://doi.org/10.1007/BF02214128
  22. Ollero M, Perez-Pe R, Muino-Blanco T, Cebrian-Perez J (1998): Improvement of ram sperm cryopreservation protocols assessed by sperm quality parameters and heterogeneity analysis. Cryobiology 37 (1):1-12. https://doi.org/10.1006/cryo.1998.2092
  23. Paulenz H, Kommisrud E, Hofmo P (2000): Effect of long‐term storage at different temperatures on the quality of liquid boar semen. Reprod Domest Anim 35(2):83-87. https://doi.org/10.1046/j.1439-0531.2000.00207.x
  24. Plisko N (1965): A method for prolonging the viability and fertilizing ability of boar spermatozoa. Svinovodstvo 19:37-41.
  25. Purdy P (2006): A review on goat sperm cryopreservation. Small Ruminant Res 63(3):215-225. https://doi.org/10.1016/j.smallrumres.2005.02.015
  26. Pursel V, Johnson L (1975): Freezing of boar spermatozoa: fertilizing capacity with concentrated semen and a new thawing procedure. J Anim Sci 40 (1):99-102. https://doi.org/10.2527/jas1975.40199x
  27. Revell S, Glossop C (1989): A long-time ambient temperature diluent for boar semen. Anim Prod 48: 579-584. https://doi.org/10.1017/S0003356100004104
  28. Roca J, Rodríguez MJ, Gil MA, Carvajal G, Garcia EM, Cuello C et al. (2005): Survival and in vitro fertility of boar spermatozoa frozen in the presence of superoxide dismutase and/or catalase. J Androl 26 (1):15-24.
  29. Johnson L, Weitze K, Fiser P, Maxwell W (2000): Storage of boar semen. Anim Reprod Sci 62(1):143-172. https://doi.org/10.1016/S0378-4320(00)00157-3
  30. Kadirvel G, Kumar S, Kumaresan A (2009): Lipid peroxidation, mitochondrial membrane potential and DNA integrity of spermatozoa in relation to intracellular reactive oxygen species in liquid and frozen- thawed buffalo semen. Anim Reprod Sci 114 (1):125-134. https://doi.org/10.1016/j.anireprosci.2008.10.002
  31. Keutsch F, Saykally R (2001): Water clusters: Untangling the mysteries of the liquid, one molecule at a time. Proc Natl Acad Sci USA 98(19):10533-10540. https://doi.org/10.1073/pnas.191266498
  32. Krems RV (2004): Breaking van der Waals molecules with magnetic fields. Phys Rev Lett 93(1):013-201.
  33. Lee SH, Song EJ, Woo JS, Lee SH, Kang HS, Cheong HE, Yang BK, Park CK (2013): Development of semen transport system for cryopreservation and fertility in bull sperm. J Emb Trans 37(3):97-102.
  34. Liu K, Brown MG, Carter C, Saykally RJ, Gregory JK, Clary DC (1996): Characterization of a cage form of the water hexamer. Nature 381(6582):501-503. https://doi.org/10.1038/381501a0
  35. Lopes S, Jurisicova A, Sun J-G, Casper RF (1998): Reactive oxygen species: potential cause for DNA fragmentation in human spermatozoa. Hum Reprod 13(4):896-900. https://doi.org/10.1093/humrep/13.4.896
  36. Lopez Rodriguez A, Rijsselaere T, Vyt P, Van Soom A, Maes D (2012): Effect of dilution temperature on boar semen quality. Reprod Domest Anim 47(5):e63-e66. https://doi.org/10.1111/j.1439-0531.2011.01938.x
  37. Maxwell W, Johnson L (1997): Membrane status of boar spermatozoa after cooling or cryopreservation. Theriogenology 48(2):209-219. https://doi.org/10.1016/S0093-691X(97)84068-X
  38. Mazor D, Golan E, Philip V, Katz M, Jafe A, Ben‐ Zvi Z et al. (1996): Red blood cell permeability to thiol compounds following oxidative stress. Eur J Haematol 57(3):241-246.
  39. Meyers S (2005): Spermatozoal response to osmotic stress. Anim Reprod Sci 89(1):57-64. https://doi.org/10.1016/j.anireprosci.2005.06.026
  40. Gadea J, Sellés E, Marco MA, Coy P, Matás C, Romar R (2004): Decrease in glutathione content in boar sperm after cryopreservation: Effect of the addition of reduced glutathione to the freezing and thawing extenders. Theriogenology 62(3):690- 701. https://doi.org/10.1016/j.theriogenology.2003.11.013
  41. Hendricks KE, Hansen PJ (2009): Can programmed cell death be induced in post-ejaculatory bull and stallion spermatozoa?. Theriogenology 71(7):1138-1146. https://doi.org/10.1016/j.theriogenology.2008.12.006
  42. Hermelo MP, Amador M, Alvarez R, Alonso A (1987): Slimming treatment efficiency and changes in serum lipids and lipoproteins in obese adolescents. Exp Clin Endocrinol Diabetes 90(06):347-360. https://doi.org/10.1055/s-0029-1210711
  43. Hu JH, Li QW, Zan LS, Jiang ZL, An JH, Wang LQ (2010): The cryoprotective effect of low-density lipoproteins in extenders on bull spermatozoa following freezing-thawing. Anim Reprod Sci 117(1):11-17. https://doi.org/10.1016/j.anireprosci.2009.04.001
  44. Inaba H, Saitou T, Tozaki K-i, Hayashi H (2004): Effect of the magnetic field on the melting transition of H[sub 2]O and D[sub 2]O measured by a high resolution and supersensitive differential scanning calorimeter. J Appl Phys 96(11):6127-6132. https://doi.org/10.1063/1.1803922
  45. Jeong BS, Yang X (2001): Cysteine, glutathione, and percoll treatments improve porcine oocyte maturation and fertilization in vitro. Mol Reprod Dev 59 (3):330-335. https://doi.org/10.1002/mrd.1038
  46. Jeyendran R, Ven H, Rachagan S, Perez‐Peieaz M, Zaneveld L (1989): Semen quality and in‐vitro fertilization. Australian-New Zealand J Obstet Gynaecol29( 2):168-172. https://doi.org/10.1111/j.1479-828X.1989.tb01710.x
  47. Jiang ZL, Li QW, Li WY, Hu JH, Zhao HW, Zhang SS (2007): Effect of low density lipoprotein on DNA integrity of freezing-thawing boar sperm by neutral comet assay. Anim Reprod Sci 99(3):401- 407. https://doi.org/10.1016/j.anireprosci.2006.08.022
  48. Johnson L, Aalbers J, Grooten H (1988): Artificial insemination of swine: fecundity of boar semen stored in Beltsville TS (BTS), Modified Modena (MM), or MR‐A and inseminated on one, three and four days after collection12. Reprod Domest Anim 23 (2):49-55. https://doi.org/10.1111/j.1439-0531.1988.tb01080.x
  49. Chang KT, Weng CI (2008): An investigation into the structure of aqueous NaCl electrolyte solutions under magnetic fields. Comput Mater Scu 43(4):1048-1055. https://doi.org/10.1016/j.commatsci.2008.02.020
  50. De Almeida M, Gazagne I, Jeulin C, Herry M, Belaisch- Allart J, Frydman R et al. (1989): In-vitro processing of sperm with autoantibodies and in-vitro fertilization results. Hum Reprod 4(1):49-53. https://doi.org/10.1093/oxfordjournals.humrep.a136844
  51. de Lamirande E, Gagnon C (1995): Impact of reactive oxygen species on spermatozoa: a balancing act between beneficial and detrimental effects. Hum Reprod 10(1):15-21. https://doi.org/10.1093/humrep/10.suppl_1.15
  52. De Leeuw F, De Leeuw A, Den Daas J, Colenbrander B, Verkleij A (1993): Effects of various cryoprotective agents and membrane-stabilizing compounds on bull sperm membrane integrity after cooling and freezing. Cryobiology 30(1):32-44. https://doi.org/10.1006/cryo.1993.1005
  53. Dontas IA, Lelovas PP, Kourkoulis SK, Aligiannis N, Paliogianni A, Mitakou S et al. (2011): Protective effect of Sideritis euboea extract on bone mineral density and strength of ovariectomized rats. Menopause 18(8):915-922. https://doi.org/10.1097/gme.0b013e31820ce580
  54. Drevet JR (2006): The antioxidant glutathione peroxidase family and spermatozoa: a complex story. Mol Cell Endocrinol 250(1):70-79. https://doi.org/10.1016/j.mce.2005.12.027
  55. DU MESNIL DBF, Dauzier L (1959): Improvement of practical use of preservation techniques for boar semen by saturation of the diluent with carbondioxide. Ann Zootech Suppl (8):81-96.
  56. Flowers W (1997): Management of boars for efficient semen production. J Reprod Fertil Suppl 52-67.
  57. Foote RH, Brockett CC, Kaproth MT (2002): Motility and fertility of bull sperm in whole milk extender containing antioxidants. Anim Reprod Sci 71 (1):13-23. https://doi.org/10.1016/S0378-4320(02)00018-0
  58. Gadea J (2003): Review: semen extenders used in the artificial inseminarion of swine. Span J Agric Res 1(2):17-27. https://doi.org/10.5424/sjar/2003012-17
  59. Alvarez JG, Storey BT (1989): Role of glutathione peroxidase in protecting mammalian spermatozoa from loss of motility caused by spontaneous lipid peroxidation. Gamete Res 23(1):77-90. https://doi.org/10.1002/mrd.1120230108
  60. Alvarez JG, Storey BT (1993): Evidence that membrane stress contributes more than lipid peroxidation to sublethal cryodamage in cryopreserved human sperm: glycerol and other polyols as sole cryoprotectant. J Androl 14(3):199-209.
  61. Barbas J, Mascarenhas R (2009): Cryopreservation of domestic animal sperm cells. Cell Tissue Banking 10(1):49-62. https://doi.org/10.1007/s10561-008-9081-4
  62. Bilodeau JF, Chatterjee S, Sirard MA, Gagnon C (2000): Levels of antioxidant defenses are decreased in bovine spermatozoa after a cycle of freezing and thawing. Mol Reprod Dev 55(3):282-288. https://doi.org/10.1002/(SICI)1098-2795(200003)55:3<282::AID-MRD6>3.0.CO;2-7
  63. Blank M, Soo L, Britten J (1976): Adsorption of albumin on rabbit sperm membranes. J Membr Biol 29(1):401-409. https://doi.org/10.1007/BF01868973
  64. Blottner S, Warnke C, Tuchscherer A, Heinen V, Torner H (2001): Morphological and functional changes of stallion spermatozoa after cryopreservation during breeding and non-breeding season. Anim Reprod Sci 65(1):75-88. https://doi.org/10.1016/S0378-4320(00)00214-1
  65. Boe-Hansen GB, Ersboll AK, Greve T, Christensen P (2005): Increasing storage time of extended boar semen reduces sperm DNA integrity. Theriogenology 63(7):2006-2019. https://doi.org/10.1016/j.theriogenology.2004.09.006
  66. Cassani P, Beconi MT, O'Flaherty C (2005): Relationship between total superoxide dismutase activity with lipid peroxidation, dynamics and morphological parameters in canine semen. Anim Reprod Sci 86(1):163-173. https://doi.org/10.1016/j.anireprosci.2004.06.006
  67. Celeghini ECC, de Arruda RP, de Andrade AFC, Nascimento J, Raphael CF, Rodrigues PHM (2008): Effects that bovine sperm cryopreservation using two different extenders has on sperm membranes and chromatin. Anim Reprod Sci 104(2):119-131. https://doi.org/10.1016/j.anireprosci.2007.02.001
  68. Aitken R, Vernet P (1997): Maturation of redox regulatory mechanisms in the epididymis. J Reprod Fertil Suppl 53:109-118.
  69. Aitken RJ (1995): Free radicals, lipid peroxidation and sperm function. Reprod Fertil Dev 7(4):659-668. https://doi.org/10.1071/RD9950659
  70. Aitken RJ, Baker MA (2004): Oxidative stress and male reprod biol. Reprod Fertil Dev 16(5):581-588. https://doi.org/10.1071/RD03089
  71. Alvarez JG, Storey BT (1982): Spontaneous lipid peroxidation in rabbit epididymal spermatozoa: its effect on sperm motility. Biol Reprod 27(5):1102-1108. https://doi.org/10.1095/biolreprod27.5.1102