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S-haplotypes and Genetic Diversity in 'Danji' Radish (Raphanus sativus L. var. hortensis)

Ahn, Yulkyun;Kim, Hyukjun;Han, Dongyeop;Park, Younghoon

  • Received : 2013.08.19
  • Accepted : 2013.11.19
  • Published : 2014.04.30

Abstract

The distribution of S-haplotypes and genetic relationships were evaluated for 47 accessions of 'Danji' radish (Raphanus sativus L. var. hortensis Baker f. gigantissimus Makino) originating from Jeju Island in South Korea. A total of 22 S-haplotype-specific SCAR markers for the S locus glycoprotein (SLG) and S receptor kinase (SRK) loci were tested, and six primer sets amplified locus-specific PCR fragments from at least one 'Danji' radish accession. S5 and S21 alleles atthe SLG locus were the most frequently distributed, and detected from 87.5% and 64.6% of the accessions, respectively. The frequency of the class-II haplotype at the SLG locus was 75%, more frequent than the class-I haplotype. The S23 allele at the SRK locus was detected from 7 accessions. Grouping of the accessions based on S-allele composition revealed three major groups, while 8 accessions showed a unique allelic composition. The genetic diversity of 47 'Danji' radishes and 1 'Gwandong' radish were also evaluated with 38 RAPD primers. A total of 312 bands were scored, and showed that 138 bands (44.2%) were monomorphic among the accessions, whereas 174 (55.8%) bands were polymorphic. Polymorphism rates ranged from 0.2 to 1.0, indicating significant variations in detecting polymorphism across RAPD primers. The genetic similarity coefficients among all pairs of the 48accessions varied from 0.62 to 0.93, and 42% of the comparisons exhibited values higher than 0.85. All the cultivars could be distinguished based on the DNA fingerprints revealed by RAPD. The comparisons between the dendrograms based on S-haplotypes and RAPDs indicate an unrelated and sporadic distribution for several accessions; however, there was a tendency for accessions with the same S-allelic composition to group into the same cluster.

Keywords

Brassicaceae;Cruciferous vegetables;DNA marker;self-incompatibility;S-locus

References

  1. Lim, S.H., K.T. Kim, S.H. Park, H.J. Cho, H.Y. Park, S.Y. Hwang, M.K. Yoon, I.G. Mok, J.G. Woo, and D.G. Oh. 2006. Identification of S haplotypes in commercial F1 hybrid cultivars of radish by PCR-RFLP analysis. Korean J. Breed. 38:167-172.
  2. Batemen, A.J. 1955. Self-incompatibility systems in angiosperms. III. Crucifierae. Heredity 9:52-68.
  3. Kong, Q., X. Li, C. Xiang, H. Wang, J. Song, and H. Zhi. 2011. Genetic diversity of radish (Raphanus sativus L.) germplasm resources revealed by AFLP and RAPD markers. Plant Mol. Biol. Rep. 29:217-223. https://doi.org/10.1007/s11105-010-0228-7
  4. Lim, S.H., H.J. Cho, S.J. Lee, Y.H. Cho, and B.D. Kim. 2002. Identification and classification of S haplotypes in Raphanus sativus by PCR-RFLP of the S locus glycoprotein (SLG) gene and the S locus receptor kinase (SRK) gene. Theor. Appl. Genet. 104:1253-1262. https://doi.org/10.1007/s00122-001-0828-6
  5. Nasrallah, J.B., T. Nishio, and M.E. Nasrallah. 1991. The selfincompatibility genes of Brassica: expression and the use in genetic ablation of floral tissues. Annu. Rev. Plant Physiol. 42:392-422.
  6. Muminovic, J., A. Merz, and A.E. Melchinger. 2005. Genetic structure and diversity among radish varieties as inferred from AFLP and ISSR analysis. J. Amer. Soc. Hort. Sci. 130:79-87.
  7. Nasrallah, M.E., J.T. Barber, and D.H. Wallace. 1970. Selfincompatibility proteins in plants: detection. Genetics and possible mode of action. Heredity 25:23-27. https://doi.org/10.1038/hdy.1970.3
  8. Nasrallah, J.B., T.H. Kao, M.L. Goldberg, and M.E. Nasrallah. 1985. A cDNA cloning encoding an S locus specific glycoprotein from Brassica oleracea. Nature 318:263-267. https://doi.org/10.1038/318263a0
  9. Rudloff, E. 1991. Results and problems in building up Selfincompatable lines and their use in hybrid seed production of winter rape (Brassica napus L.). Proc. 8th Int. Rapeseed Cong., Saskatoon p. 107-112.
  10. Takayama, S., H. Shimosato, H. Shiba, M. Funato, F.S. Che, M. Watanabe, M. Iwano, and A. Isogai. 2001. Direct ligand-receptor complex interaction controls Brasscia self-incompatibility. Nature 413:534-538. https://doi.org/10.1038/35097104
  11. Nasrallah, J.B. and M.E. Nasrallah. 1993. Pollen-stigma interaction in the sporophytic self-incompatibility response. Plant Cell 5:1325-1335.
  12. Watanabe, M., A. Ito, Y. Takada, C. Ninomiya, T. Kakizaki, Y. Takahata, K. Hatakeyama, K. Hinata, G. Suzuki, T. Takasaki, Y. Satta, H. Shiba, S.Takayama, and A. Isogai. 2000. Highly divergent sequences of the pollen self-incompatibility (S) gene in class-1 S haplotypes of Brassica campestris (syn. Rapa) L. FEBS Lett. 473:139-144. https://doi.org/10.1016/S0014-5793(00)01514-3