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Effect of DMfree (GTE) on Gene Array Profile of M. leprae Infected Mesenchymal Stem Cells

디엠프리(녹차 추출물)가 나균 감염 중간엽 줄기세포의 유전자 발현에 미치는 영향

  • Park, Ran-Sook (Dept. of Food & Nutrition, Soongeui Women's College)
  • 박란숙 (숭의여자대학교 식품영양과)
  • Received : 2014.02.28
  • Accepted : 2014.04.10
  • Published : 2014.04.30

Abstract

This study found antibacterial activity of $DMfree^{(R)}$ [green tea extract] on facultative bacteria by direct petri dish method and gene array of obligatory M. leprae infected mesenchymal stem cells (MSC). While DMfree showed DPPH radical scavenging effect and high contents of polyphenol, it did not inhibit growth of facultative bacteria such as E. coli and S. aureus on the petri dish. The result does not exclude a possible antibacterial effect of organic solvent extract of green tea rather than DMfree which comes from the water extract of green tea. Pre-treatment of DMfree appeared to have no effect on copy number of 14 genes compared with control MSC by real-time RT-PCR. However pre-treatment of DMfree on M. leprae infected MSC revealed a significant decrease of anti-inflammatory cytokine (IL-6), (P<0.038) and sharp down-regulation of pro-inflammatory cytokine (IL-1). Enhanced expression of VEGFR-1 mRNA was noted in DMfree pretreated M. leprae infected MSC group (P<0.003). These results show that DMfree would stabilize M. leprae infected MSC from further inflammation by down-regulating anti-inflammatory cytokine (IL-6) and pro-inflammatory cytokine (IL-$1{\beta}$). This is the first report on DMfree inhibition of IL-6 and IL-$1{\beta}$ expression in M. leprae infected MSC. Further experiments that detect protein levels of IL-$1{\beta}$ and IL-6 may support the result of this gene array.

Acknowledgement

Supported by : 숭의여자대학

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  1. DMfree®(Green Tea Extract) Inhibits IL-6 of Mycobacterium leprae Infected Mesenchymal Stem Cells vol.28, pp.4, 2015, https://doi.org/10.9799/ksfan.2015.28.4.695