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Enterotoxin Productivity and Antimicrobial Susceptibility of Bacillus cereus BY06 Isolated from Pigs with Diarrheal Disease

자돈 설사 분변에서 분리한 Bacillus cereus BY06의 장 독소 생성 및 항균제 감수성

  • Wu, Wei-Jie (Dept. of Food Science & Biotechnology, Chonbuk National University) ;
  • Rho, Youg-Hwan (Dept. of Food Science & Biotechnology, Chonbuk National University) ;
  • Ahn, Byung-Yong (Dept. of Oriental Medicine Resources, Chonbuk National University)
  • 오위걸 (전북대학교 바이오식품공학과) ;
  • 노용환 (전북대학교 바이오식품공학과) ;
  • 안병용 (전북대학교 한약자원학과)
  • Received : 2013.11.21
  • Accepted : 2014.04.03
  • Published : 2014.04.30

Abstract

The enterotoxin production and antimicrobial susceptibility on hemolytic strains from stools of diarrheal pigs was investigated in this study. Through morphological observation, gyrB nucleotide sequence, and API kit analysis, the selected potential pathogenic strain BY06 was identified as Bacillus cereus. Because the characteristic of enterotoxin symptoms were widely caused by Bacillus cereus strains, a PCR test was carried out in order to check the enterotoxin genes (hblA) in this strain. According to the results, this strain was an enterotoxin positive strain containing the hblA gene. The minimum inhibitory concentrations of 10 different antimicrobial agents were screened by the agar dilution test, indicating that this strain was resistant to penicillin G and intermediate to erythromycin; however, it susceptible to cephalothin, vancomycin, clindamycin, fusidic acid, gentamicin, ciprofloxacin, tetracycline and rifampin. These results suggest that the B. cereus BY06 isolated from pig feces has a potential risk of producing enterotoxin and is resistant to penicillin G, but susceptible to various antimicrobial agents.

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