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Antioxidant Activities of Amaranth (Amaranthus spp. L.) Flower Extracts

아마란스 꽃 추출물의 항산화에 관한 연구

  • Jo, Hyeon-Ju (Dept. of Food Science and Technology, Seoul National University of Science & Technology) ;
  • Kim, Jeong Won (Q&A Korea Corperation) ;
  • Yoon, Jin-A (Dept. of Food & Nutrition, Baewha Women's University) ;
  • Kim, Kyoung Im (Dept. of Hotel Baking Technology, Hyejeon College) ;
  • Chung, Kang-Hyun (Dept. of Food Science and Technology, Seoul National University of Science & Technology) ;
  • Song, Byeong Chun (Division of Food Bioscience, Konkuk University) ;
  • An, Jeung Hee (Division of Food Bioscience, Konkuk University)
  • 조현주 (서울과학기술대학교 식품공학과) ;
  • 김정원 ((주)큐앤에이코리아) ;
  • 윤진아 (배화여자대학교 식품영양과) ;
  • 김경임 (혜전대학교 호텔조리외식계열) ;
  • 정강현 (서울과학기술대학교 식품공학과) ;
  • 송병춘 (건국대학교 식품생명과학부) ;
  • 안정희 (건국대학교 식품생명과학부)
  • Received : 2013.11.21
  • Accepted : 2014.04.01
  • Published : 2014.04.30

Abstract

This study investigates the free radical-scavenging activities of Amaranth (Amaranthus spp. L.) red and purple flower extracts. The methanol and hot water extracts of flower are being evaluated for its total polyphenol and flavonoid contents, scavenging activities by the DPPH and ABTS analysis, SOD-like activity, and inhibition activities of superoxide radical on the HL-60 cells and nitric oxide of the RAW 264.7 cells. The PFM (purple flower extracted with MeOH) showed the highest total phenolic and flavonoid content, 606.95 mg GAE/100 g and 254.69 mg CE/100 g, respectively. Amongst the scavenging activities of the DPPH radicals, PFM($RC_{50}=155.06{\mu}g/m{\ell}$) is the highest of all the samples. The ABTS radical-scavenging activity is also highest for PFM (53.16%) at the $250{\mu}g/m{\ell}$ concentration. But, the SOD-like activity of the PFW (purple flower extracted with hot water) increases more than 3 folds of the PFM. In the leukemia HL-60 cell, the PFM shows strongly inhibited superoxide radical generations at a concentration of $200{\mu}g/m{\ell}$ at 72.34%, which increases with 1.79 folds more than the RFW (red flower extracted with hot water). The inhibition activity of nitric oxide in Raw 264.7 cells is the highest for PMF (46.90%) at a $250{\mu}g/m{\ell}$ concentration. In conclusion, PMF show the highest flavonoid contents and the most powerful free radical-scavenging activity. Our results suggest that the increase of antioxidant activities depend on flavonoid contents. Thus, Amaranth flower can be useful for natural antioxidant compounds.

Acknowledgement

Supported by : (주)큐앤에이코리아

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