DOI QR코드

DOI QR Code

In vitro Antioxidant Activities of Trianthema portulacastrum L. Hydrolysates

  • Yaqoob, Sadaf (Department of Chemistry and Biochemistry, University of Agriculture) ;
  • Sultana, Bushra (Department of Chemistry and Biochemistry, University of Agriculture) ;
  • Mushtaq, Muhammad (Department of Chemistry and Biochemistry, University of Agriculture)
  • Received : 2013.12.04
  • Accepted : 2014.03.10
  • Published : 2014.03.31

Abstract

Hydrolysates of Trianthema portulacastrum in acidified methanol were evaluated for their total phenolic (TP) constituents and respective antioxidant activities using in vitro assays (i.e., 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, percent inhibition of linoleic acid peroxidation, and ferric reducing power). The observed results indicate that root, shoot, and leaf fractions of T. portulacastrum contain 50.75~98.09 mg gallic acid equivalents/g dry weight of TP. In addition, these fractions have substantial reducing potentials (0.10~0.59), abilities to inhibit peroxidation (43.26~89.98%), and DPPH radical scavenging capabilities ($6.98{\sim}311.61{\mu}g/mL$ $IC_{50}$). The experimental data not only reveal T. portulacastrum as potential source of valuable antioxidants, but also indicate that acidified methanol may be an ideal choice for the enhanced recovery of phenolic compounds with retained biological potential for the food and pharmaceutical industry.

References

  1. Sultana B, Hussain Z, Asif M, Munir A. 2012. Investigation on the antioxidant activity of leaves, peels, stems bark, and kernel of mango (Mangifera indica L.). J Food Sci 77: C849-C852. https://doi.org/10.1111/j.1750-3841.2012.02807.x
  2. Dashtban M, Maki M, Leung KT, Mao C, Qin W. 2010. Cellulase activities in biomass conversion: measurement methods and comparison. Crit Rev Biotechnol 30: 302-309. https://doi.org/10.3109/07388551.2010.490938
  3. Ajila CM, Brar SK, Verma M, Tyagi RD, Godbout S, Valero JR. 2011. Extraction and analysis of polyphenols: recent trends. Crit Rev Biotechnol 31: 227-249. https://doi.org/10.3109/07388551.2010.513677
  4. Anwar F, Latif S, Przybylski R, Sultana B, Ashraf M. 2007. Chemical composition and antioxidant activity of seeds of different cultivars of mungbean. J Food Sci 72: S503-S510.
  5. Ali-Emmanuel N, Moudachirou M, Akakpo JA, Quetin- Leclercq J. 2003. Treatment of bovine dermatophilosis with Senna alata, Lantana camara and Mitracarpus scaber leaf extracts. J Ethnopharmacol 86: 167-171. https://doi.org/10.1016/S0378-8741(03)00054-0
  6. Muanda F, Kone D, Dicko A, Soulimani R, Younos C. 2011. Phytochemical composition and antioxidant capacity of three malian medicinal plant parts. Evid Based Complement Alternat Med 2011: 674320.
  7. Gousiadou C, Gotfredsen CH, Matsa M, Hadjipavlou-Litina D, Skaltsa H. 2013. Minor iridoids from Scutellaria albida ssp. albida. Inhibitory potencies on lipoxygenase, linoleic acid lipid peroxidation and antioxidant activity of iridoids from Scutellaria sp.. J Enzyme Inhib Med Chem 28: 704-710. https://doi.org/10.3109/14756366.2012.672415
  8. Aaby K, Ekeberg D, Skrede G. 2007. Characterization of phenolic compounds in strawberry (Fragaria $\times$ ananassa) fruits by different HPLC detectors and contribution of individual compounds to total antioxidant capacity. J Agric Food Chem 55: 4395-4406. https://doi.org/10.1021/jf0702592
  9. Ilahi I, Samar S, Khan I, Ahmad I. 2013. In vitro antioxidant activities of four medicinal plants on the basis of DPPH free radical scavenging. Pak J Pharm Sci 26: 949-952.
  10. Rababah TM, Banat F, Rababah A, Ereifej K, Yang W. 2010. Optimization of extraction conditions of total phenolics, antioxidant activities, and anthocyanin of oregano, thyme, terebinth, and pomegranate. J Food Sci 75: C626-C632. https://doi.org/10.1111/j.1750-3841.2010.01756.x
  11. Raudonis R, Raudone L, Jakstas V, Janulis V. 2012. Comparative evaluation of post-column free radical scavenging and ferric reducing antioxidant power assays for screening of antioxidants in strawberries. J Chromatogr A 1233: 8-15. https://doi.org/10.1016/j.chroma.2012.02.019
  12. Keegstra K. 2010. Plant cell walls. Plant Physiol 154: 483-486. https://doi.org/10.1104/pp.110.161240
  13. Kumar R, Singh S, Singh OV. 2008. Bioconversion of lignocellulosic biomass: biochemical and molecular perspectives. J Ind Microbiol Biotechnol 35: 377-391. https://doi.org/10.1007/s10295-008-0327-8
  14. Komes D, Belscak-Cvitanovic A, Horzic D, Rusak G, Likic S, Berendika M. 2011. Phenolic composition and antioxidant properties of some traditionally used medicinal plants affected by the extraction time and hydrolysis. Phytochem Anal 22: 172-180. https://doi.org/10.1002/pca.1264
  15. Corral-Aguayo RD, Yahia EM, Carrillo-Lopez A, Gonzalez-Aguilar G. 2008. Correlation between some nutritional components and the total antioxidant capacity measured with six different assays in eight horticultural crops. J Agric Food Chem 56: 10498-10504. https://doi.org/10.1021/jf801983r
  16. Roy MK, Koide M, Rao TP, Okubo T, Ogasawara Y, Juneja LR. 2010. ORAC and DPPH assay comparison to assess antioxidant capacity of tea infusions: relationship between total polyphenol and individual catechin content. Int J Food Sci Nutr 61: 109-124. https://doi.org/10.3109/09637480903292601
  17. Sanchez-Gallego JI, Lopez-Revuelta A, Hernandez-Hernandez A, Sardina JL, Lopez-Ruano G, Sanchez-Yague J, Llanillo M. 2011. Comparative antioxidant capacities of quercetin and butylated hydroxyanisole in cholesterol-modified erythrocytes damaged by tert-butylhydroperoxide. Food Chem Toxicol 49: 2212-2221. https://doi.org/10.1016/j.fct.2011.06.014
  18. Anwar F, Kalsoom U, Sultana B, Mushtaq M, Mehmood T, Arshad HA. 2013. Effect of drying method and extraction solvent on the total phenolics and antioxidant activity of cauliflower (Brassica oleracea L.) extracts. Int Food Res J 20:653-659
  19. Ahn CB, Shin TS, Seo HK, Je JY. 2012. Phenolic composition and antioxidant effect of aqueous extract of Arisaema cum Bile, the oriental herb medicine, in human fibroblast cells. Immunopharmacol Immunotoxicol 34: 661-666. https://doi.org/10.3109/08923973.2011.649289
  20. Apak R, Guclu K, Demirata B, Ozyurek M, Celik SE, Bektasoglu B, Berker KI, Ozyurt D. 2007. Comparative evaluation of various total antioxidant capacity assays applied to phenolic compounds with the CUPRAC assay. Molecules 12: 1496-1547. https://doi.org/10.3390/12071496
  21. Zhou C, Sun C, Chen K, Li X. 2011. Flavonoids, phenolics, and antioxidant capacity in the flower of Eriobotrya japonica Lindl. Int J Mol Sci 12: 2935-2945. https://doi.org/10.3390/ijms12052935
  22. Tiveron AP, Melo PS, Bergamaschi KB, Vieira TMFS, Regitano-d'Arce MAB, Alencar SM. 2012. Antioxidant activity of Brazilian vegetables and its relation with phenolic composition. Int J Mol Sci 13: 8943-8957. https://doi.org/10.3390/ijms13078943
  23. Sultana B, Anwar F, Ashraf M. 2009. Effect of extraction solvent/technique on the antioxidant activity of selected medicinal plant extracts. Molecules 14: 2167-2180. https://doi.org/10.3390/molecules14062167
  24. Alvarez-Suarez JM, Tulipani S, Diaz D, Estevez Y, Romandini S, Giampieri F, Damiani E, Astolfi P, Bompadre S, Battino M. 2010. Antioxidant and antimicrobial capacity of several monofloral Cuban honeys and their correlation with color, polyphenol content and other chemical compounds. Food Chem Toxicol 48: 2490-2499. https://doi.org/10.1016/j.fct.2010.06.021
  25. Ahmed M, Sorifa Akter MST, Eun JB. 2011. Optimization conditions for anthocyanin and phenolic content extraction form purple sweet potato using response surface methodology. Int J Food Sci Nutr 62: 91-96. https://doi.org/10.3109/09637486.2010.511167
  26. Kumar G, Banu GS, Pappa PV, Sundararajan M, Pandian MR. 2004. Hepatoprotective activity of Trianthema portulacastrum L. against paracetamol and thioacetamide intoxication in albino rats. J Ethnopharmacol 92: 37-40. https://doi.org/10.1016/j.jep.2003.12.009
  27. Bhattacharya S, Chatterjee M. 1998. Trianthema portulacastrum restores the antioxidant defense enzyme levels and hepatic biotransformation patterns in experimental rat hepatocarcinogenesis. Ital J Biochem 47: 225-232.
  28. Agostini F, Bertussi RA, Agostini G, Atti Dos Santos AC, Rossato M, Vanderlinde R. 2012. Supercritical extraction from vinification residues: fatty acids, $\alpha$-tocopherol, and phenolic compounds in the oil seeds from different varieties of grape. Sci World J 2012: 790486.
  29. Beejmohun V, Fliniaux O, Grand E, Lamblin F, Bensaddek L, Christen P, Kovensky J, Fliniaux MA, Mesnard F. 2007. Microwave-assisted extraction of the main phenolic compounds in flaxseed. Phytochem Anal 18: 275-282. https://doi.org/10.1002/pca.973
  30. Bimakr M, Rahman RA, Taip FS, Adzahan NM, Sarker MZ, Ganjloo A. 2012. Optimization of ultrasound-assisted extraction of crude oil from winter melon (Benincasa hispida) seed using response surface methodology and evaluation of its antioxidant activity, total phenolic content and fatty acid composition. Molecules 17: 11748-11762. https://doi.org/10.3390/molecules171011748
  31. Carrera C, Ruiz-Rodriguez A, Palma M, Barroso CG. 2012. Ultrasound assisted extraction of phenolic compounds from grapes. Anal Chim Acta 732: 100-104. https://doi.org/10.1016/j.aca.2011.11.032
  32. Balyan RS, Bhan VM. 1986. Germination of horse purslane (Trianthema portulacastrum) in relation to temperature, storage conditions, and seeding depths. Weed Sci 34: 513-515. https://doi.org/10.1017/S0043174500067333
  33. Shivhare MK, Singour PK, Chaurasiya PK, Pawar RS. 2012. Trianthema portulacastrum Linn. (Bishkhapra). Pharmacogn Rev 6: 132-140. https://doi.org/10.4103/0973-7847.99947
  34. Sharmila Banu G, Kumar G, Murugesan AG. 2009. Effect of ethanolic leaf extract of Trianthema portulacastrum L. on aflatoxin induced hepatic damage in rats. Indian J Clin Biochem 24: 414-418. https://doi.org/10.1007/s12291-009-0074-2

Cited by

  1. Trianthema portulacastrum L. (giant pigweed): phytochemistry and pharmacological properties vol.16, pp.3, 2017, https://doi.org/10.1007/s11101-017-9493-5
  2. Enzyme-assisted supercritical fluid extraction of phenolic antioxidants from pomegranate peel vol.104, 2015, https://doi.org/10.1016/j.supflu.2015.05.020
  3. Health-promoting and disease-preventive potential of Trianthema portulacastrum Linn. (Gadabani)—An Indian medicinal and dietary plant vol.14, pp.2, 2016, https://doi.org/10.1016/S2095-4964(16)60247-9
  4. Maceration mediated liquid–liquid extraction of conjugated phenolics from spent black tea leaves extraction of non-extractable phenolics vol.10, pp.35, 2018, https://doi.org/10.1039/C8AY01350K
  5. Salting-out-assisted liquid–liquid extraction and reverse-phase high-performance liquid chromatographic monitoring of thiacloprid in fruits and vegetables vol.53, pp.10, 2018, https://doi.org/10.1080/01496395.2017.1417317