DOI QR코드

DOI QR Code

Anti-oxidizing effect of the dichloromethane and hexane fractions from Orostachys japonicus in LPS-stimulated RAW 264.7 cells via upregulation of Nrf2 expression and activation of MAPK signaling pathway

  • Lee, Hyeong-Seon ;
  • Lee, Gyeong-Seon ;
  • Kim, Seon-Hee ;
  • Kim, Hyun-Kyung ;
  • Suk, Dong-Hee ;
  • Lee, Dong-Seok
  • Received : 2013.04.19
  • Accepted : 2013.06.13
  • Published : 2014.02.28

Abstract

Orostachys japonicus shows various biological activities. However, the molecular mechanisms remain unknown in LPS-stimulated macrophages. Here, we investigated the anti-oxidizing effect of the dichloromethane (DCM) and hexane fractions from O. japonicus (OJD and OJH) against oxidative stress in RAW 264.7 cells stimulated by LPS. OJD and OJH significantly increased the expression of heme oxygenase-1 (HO-1) in a dose- and time-dependent manner. Additionally, it was found that the expression of HO-1 was stimulated by Nrf2 activated via degradation of Keap1. ERK and p38 inhibitors repressed HO-1 induced by OJD and OJH in LPS-stimulated cells, respectively. In conclusion, these results suggest that OJD and OJH may block oxidative damage stimulated by LPS, via increasing the expression of HO-1 and Nrf2, and MAPK signaling pathway.

Keywords

Anti-oxidizing effect;HO-1;MAPK;Nrf2/Keap1;Orostachys japonicus

References

  1. Kim, I. D. and Ha, B. J. (2009) Paeoniflorin protects RAW 264.7 macrophages from LPS-induced cytotoxicity and genotoxicity. Toxicol. In Vitro. 23, 1014-1019. https://doi.org/10.1016/j.tiv.2009.06.019
  2. Chow, J. M., Shen, S. C., Huan, S. K., Lin, H. Y. and Chen, Y. C. (2005) Quercetin, but not rutin and quercitrin, prevention of H2O2-induced apoptosis via anti-oxidant activity and heme oxygenase 1 gene expression in macrophages. Biochem. Pharmacol. 69, 1839-1851. https://doi.org/10.1016/j.bcp.2005.03.017
  3. Kim, C. K., Jang, J. S., Cho, M. R., Agarawal, S. R. and Cha, Y. N. (2010) Taurine chloramine induces heme oxygenase-1 expression via Nrf2 activation in murine macrophages. Int. Immunopharmacology 10, 440-446. https://doi.org/10.1016/j.intimp.2009.12.018
  4. Lee, D. S., Jeong, G. S., Li, B., Park, H. and Kim, Y. C. (2010) Anti-inflammatory effects of sulfuretin from Rhus verniciflua Stokes via the induction of heme oxygenase-1 expression in murine macrophages. Int. Immunopharmacology 10, 850-858. https://doi.org/10.1016/j.intimp.2010.04.019
  5. Kim, J. Y., Cha, Y. N. and Surh, Y. J. (2010) Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis. Mutat. Res. 690, 12-23. https://doi.org/10.1016/j.mrfmmm.2009.09.007
  6. Park, S. Y., Park, D. J., Kim, Y. H., Kim, Y. H., Choi, Y. W. and Lee, S. J. (2011) Schisandra chinensis $\alpha$-iso-cubebenol induces heme oxygenase-1 expression through PI3K/ Akt and Nrf2 signaling and has anti-inflammatory activity in Porphyromonas gingivalis lipopolysaccharide-stimulated macrophages. Int. Immunopharmacology 11, 1907-1915. https://doi.org/10.1016/j.intimp.2011.07.023
  7. Park, J. C., Jan, W. D., Park, J. R., Choi, S. H. and Choi, J. W. (2005) Changes in hepatic drug metabolizing enzymes and lipid peroxidation by methanol extract and major compound of Orostachys japonicus. J. Ethnopharmacol. 102, 313-318. https://doi.org/10.1016/j.jep.2005.06.023
  8. Khlifi, D., Sghaier, R. M., Amouri, S., Laouini, D., Hamdi, M. and Bouajila, J. (2013) Composition and anti-oxidant, anti- cancer and anti-inflammatory activities of Artemisia herba- alba, Ruta chalpensis L. and Peganum harmala L. Food Chem. Toxicol. 55, 202-208. https://doi.org/10.1016/j.fct.2013.01.004
  9. Ryu, D. S., Baek, G. O., Kim, E. Y., Kim, K. H. and Lee, D. S. (2011) Effects of polysaccharides derived from Orostachys japonicus on induction of cell cycle arrest and apoptotic cell death in human colon cancer cells. BMB Rep. 43, 750-755. https://doi.org/10.5483/BMBRep.2010.43.11.750
  10. Jeong, J. H., Ryu, D. S., Suk, D. H. and Lee, D. S. (2011) Anti-inflammatory effects of ethanol extract from Orostachys japonicus on modulation of signal pathways in LPSstimulated RAW 264.7 cells. BMB Rep. 44, 399-404. https://doi.org/10.5483/BMBRep.2011.44.6.399
  11. Lee, H. S., Ryu, D. S., Lee, G. S. and Lee, D. S. (2012) Antiinflammatory effects of dichloromethane fraction from Orostachys japonicus in RAW 264.7 cells: Suppression of NF-${\kappa}B$ activation and MAPK signaling. J. Ethnopharmacology 140, 271-276. https://doi.org/10.1016/j.jep.2012.01.016
  12. Lee, H. S., Bilehal, D., Lee, G. S., Ryu, D. S., Kim, H. K., Suk, D. H. and Lee, D. S. (2013) Anti-inflammatory effect of the hexane fraction from Orostachys japonicus in RAW 264.7 cells by suppression of NF-${\kappa}B$ and PI3K-Akt signaling. J. Funct. Foods 5, 1217-1225. https://doi.org/10.1016/j.jff.2013.04.004
  13. Ma, Y. F., Jung, J. Y., Jung, Y. J., Choi, J. H., Jeong, W. S., Song, Y. S., Kang, J. S., Bi, K. and Kim, M. J. (2009) Anti-inflammatory Activity of Coumarins Isolated from Angelica gigas Nakai on LPS-stimulated RAW 264.7 Cells. J. Food Sci. Nut. 14, 179-187. https://doi.org/10.3746/jfn.2009.14.3.179
  14. Copple, I. M., Goldring, C. E., Kitteringham, N. R. and Park, B. K. (2008) The Nrf2-Keap1 defence pathway: Role in protection against drug-induced toxicity. Toxicology 246, 24-33. https://doi.org/10.1016/j.tox.2007.10.029
  15. Chung, H. T., Choi, B. M., Kwon, Y. G. and Kim, Y. M. (2008) Interaction Relations between Nitric Oxide (NO) and Carbon Monoxide (CO): Heme Oxygenase-1/CO pathway is a key modulator in NO-mediated Antiapoptosis and Anti-inflammation. Methods Enzymol. 441, 329-338. https://doi.org/10.1016/S0076-6879(08)01218-4
  16. Kim, A. N., Jeon, W. K., Lee, J. J. and Kim, B. C. (2010) Up-regulation of heme oxygenase-1 expression through CaMKII-ERK1/2-Nrf2 signaling mediates the anti-inflammatory effect of bisdemethoxycurcumin in LPS-stimulated macrophages. Free Radic. Biol. Med. 49, 323-331. https://doi.org/10.1016/j.freeradbiomed.2010.04.015
  17. Song, J. D., Kim, K. M., Kim, J. M., Yoo, Y. H. and Park, Y. C. (2011) Involvement of Peroxynitrite in NO Doner- Induced HO-1 Expression in Rat Articular Chondrocytes. J. Life Sci.21, 486-493. https://doi.org/10.5352/JLS.2011.21.4.486
  18. Kim B. C., Choi, J. W., Hong, H. Y., Lee, S. A., Hong, S., Park, E. H., Kim, S. J. and Lim C. J. (2006) Heme oxygenase-1 mediates the anti-inflammatory effect of mushroom Phellinus linteus in LPS-stimulated RAW 264.7 macrophages. J. Ethnopharmacol. 106, 364-371. https://doi.org/10.1016/j.jep.2006.01.009
  19. Fan, W., Tang, Z., Chen, D., Moughon, D., Ding, X., Chen, S., Zhu, M. and Zhong, Q. (2010) Keap1 facilitates p62- mediated ubiquitin aggregate clearance via autophage. Autophagy 6, 614-621. https://doi.org/10.4161/auto.6.5.12189
  20. Park, S. Y., Park, D. J., Kim, Y. H., Kim, Y. H., Kim, S. G., Shon, K. J., Choi, Y. W. and Lee, S. J. (2011) Upregulation of heme oxygenase-1 via PI3K/Akt and Nrf2 signaling pathways mediates the anti-inflammatory activity of Schisandrin in Porphyromonas gingivalis LPS-stimulated macrophages. Immunol. Lett. 139, 93-101. https://doi.org/10.1016/j.imlet.2011.05.007
  21. Lee, J. M. and Johnson, J. A. (2004) An Important Role of Nrf2-ARE Pathway in the Cellular Defense Mechanism. J. Biochem. Mol. Biol. 37, 139-143. https://doi.org/10.5483/BMBRep.2004.37.2.139
  22. Stepkowski, T. M. and Kruszewski, M. K. (2011) Molecular cross-talk between the NRF2/KEAP1 signaling pathway, autophagy, and apoptosis. Free Radic. Biol. Med. 50, 1186-1195. https://doi.org/10.1016/j.freeradbiomed.2011.01.033
  23. Manzoor, Z. and Koh, Y. S. (2012) Mitogen-activated Protein Kinases in Inflammation. J. Bacteriol. Virol. 42, 189-195. https://doi.org/10.4167/jbv.2012.42.3.189
  24. Cho, J. E., Park, S., Lee, H., Cho, S. N. and Kim, Y. S. (2013) Mycobacterium tuberculosis-induced expression of granulocyte- macrophage colony stimulating factor is mediated by PI3-K/MEK1/p38 MAPK signaling pathway. BMB Rep. 46, 213-218. https://doi.org/10.5483/BMBRep.2013.46.4.200

Cited by

  1. Nrf2-Mediated HO-1 Induction Contributes to Antioxidant Capacity of a Schisandrae Fructus Ethanol Extract in C2C12 Myoblasts vol.6, pp.12, 2014, https://doi.org/10.3390/nu6125667
  2. Induction of glutathione synthesis in human hepatocytes by acute and chronic arsenic exposure: Differential roles of mitogen-activated protein kinases vol.325, 2014, https://doi.org/10.1016/j.tox.2014.09.002
  3. Dimethyl Fumarate Protects Neural Stem/Progenitor Cells and Neurons from Oxidative Damage through Nrf2-ERK1/2 MAPK Pathway vol.16, pp.6, 2015, https://doi.org/10.3390/ijms160613885
  4. The role of macrophages in the susceptibility of Fc gamma receptor IIb deficient mice to Cryptococcus neoformans vol.7, 2017, https://doi.org/10.1038/srep40006
  5. Celastrol attenuates angiotensin II mediated human umbilical vein endothelial cells damage through activation of Nrf2/ERK1/2/Nox2 signal pathway vol.797, 2017, https://doi.org/10.1016/j.ejphar.2017.01.027
  6. Protection Effect of Punicalagin Isolated from Pomegranate on Inflammation and Ethanol-induced Gastric Mucosal Injury vol.37, pp.11, 2016, https://doi.org/10.1002/bkcs.10973
  7. Glutathione Protects Brain Endothelial Cells from Hydrogen Peroxide-Induced Oxidative Stress by Increasing Nrf2 Expression vol.23, pp.1, 2014, https://doi.org/10.5607/en.2014.23.1.93
  8. The inhibitory effect of Orostachys japonicus on dextran sulfate sodium-induced colitis in mice vol.29, pp.4, 2014, https://doi.org/10.6116/kjh.2014.29.4.29
  9. Anti-inflammatory potential of saponins derived from cultured wild ginseng roots in lipopolysaccharide-stimulated RAW 264.7 macrophages vol.35, pp.6, 2015, https://doi.org/10.3892/ijmm.2015.2165
  10. Nrf2 Expression and Apoptosis in Quercetin-treated Malignant Mesothelioma Cells vol.38, pp.5, 2015, https://doi.org/10.14348/molcells.2015.2268
  11. ASIC2a-dependent increase of ASIC3 surface expression enhances the sustained component of the currents vol.49, pp.10, 2016, https://doi.org/10.5483/BMBRep.2016.49.10.057
  12. Protective Effect ofArtemisia asiaticaExtract and Its Active Compound Eupatilin against Cisplatin-Induced Renal Damage vol.2015, 2015, https://doi.org/10.1155/2015/483980
  13. Pyrrole-Derivative of Chalcone, (E)-3-Phenyl-1-(2-Pyrrolyl)-2-Propenone, Inhibits Inflammatory Responses via Inhibition of Src, Syk, and TAK1 Kinase Activities vol.24, pp.6, 2016, https://doi.org/10.4062/biomolther.2016.027
  14. Ethanol extract of Prunus mume fruit attenuates hydrogen peroxide-induced oxidative stress and apoptosis involving Nrf2/HO-1 activation in C2C12 myoblasts vol.26, pp.2, 2016, https://doi.org/10.1016/j.bjp.2015.06.012