Isolation and Charaterization of Dye-Degrading Microorganisms for Treatment of Chromaticity Contained in Industrial Dyeing Wastewater

염색공단폐수의 색도처리를 위한 염료분해 균주의 분리와 특성

  • Kim, Jung Tae (Department of Environmental System, Pusan National University) ;
  • Park, Guen Tae (Institute for Research & Industry Cooperation, Pusan National University) ;
  • Lee, Geon (Research Department, Dongchang Corporation) ;
  • Kang, Kyeong Hwan (Department of Biotechnology and Bioengineering, Pukyong National University) ;
  • Kim, Joong Kyun (Department of Biotechnology and Bioengineering, Pukyong National University) ;
  • Lee, Sang Joon (Department of Microbiology, Pusan National University)
  • Received : 2013.08.13
  • Accepted : 2013.12.18
  • Published : 2014.01.31


To treat chromaticity contained in effluents of dyeing wastewater efficiently, potent dye-degrading microorganisms were isolated from influent water, aeration- tank sludge, recycle water and settling-tank sludge located in leather and dyeing treatment plant. Six potent strains were finally isolated and identified as Comamonas testosteroni, Methylobacteriaceae bacterium, Stenotrophomonas sp., Kluyveromyces fragilis, Ascomycetes sp. and Basidiomycetes sp. When Basidiomycetes sp. was inoculated into ME medium containing basal mixed-dyes, 93% of color was removed after 8 days incubation. In the same experiment, the 1:1 mixed culture of Basidiomycetes sp. and photosynthetic bacterium exhibited 88% of color removal; however, it showed better color removal for single-color dyes. The aeration-tank and settling-tank samples revealed higher color removal (95-96%) for black dyes. The settling-tank sample also revealed higher color removal on basal mixed-dyes, which resulted in 90% color removal after 6-h incubation. From the above results, it is expected to achieve a higher color removal using the mixed microorganisms that were isolated from aeration-tank and settling-tank samples.


Dyeing wastewater;Biodegradation;Dye-degrading microorganisms;Chromaticity removal


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