DOI QR코드

DOI QR Code

Screening of Biological Activity of Solvent Extract from Styela clava Tunic for Fishery Waste Recycling

수산폐기물 재활용을 위한 미더덕껍질 용매 추출물의 생리활성 탐색

  • Lee, Sang-Mee (College of Natural Resources & Life Science, Pusan National University) ;
  • Kang, Eun-Jin (College of Natural Resources & Life Science, Pusan National University) ;
  • Go, Tae-Hun (College of Natural Resources & Life Science, Pusan National University) ;
  • Jeong, Seong-Yun (Department of Medical Life Science, Catholic University of Daegu) ;
  • Park, Geun-Tae (Research and University-Industry Cooperation, Pusan National University) ;
  • Lee, Hee-Sup (College of Human Ecology, Pusan National University) ;
  • Hwang, Dae-Youn (College of Natural Resources & Life Science, Pusan National University) ;
  • Jung, Young-Jin (College of Natural Resources & Life Science, Pusan National University) ;
  • Son, Hong-Joo (College of Natural Resources & Life Science, Pusan National University)
  • 이상미 (부산대학교 생명자원과학대학) ;
  • 강은진 (부산대학교 생명자원과학대학) ;
  • 고태훈 (부산대학교 생명자원과학대학) ;
  • 정성윤 (대구가톨릭대 의생명과학과) ;
  • 박근태 (부산대학교 산학협력단) ;
  • 이희섭 (부산대학교 생활환경대학) ;
  • 황대연 (부산대학교 생명자원과학대학) ;
  • 정영진 (부산대학교 생명자원과학대학) ;
  • 손홍주 (부산대학교 생명자원과학대학)
  • Received : 2013.07.02
  • Accepted : 2013.08.12
  • Published : 2014.01.31

Abstract

Styela clava tunic is generated in large amounts as a waste from S. clava processing plants and causes environmental problem. Although biological activities of S. clava were reported by many investigators, study on S. clava tunic was little. In this study, therefore, tyrosinase inhibition and antioxidative activities of extracts from S. clava tunic using different solvent were investigated for recycling of the fishery waste. Among extraction methods tested, autoclaved extraction (25.7%) and hot water extraction (18.2%) appeared to be effective for extraction. The highest total phenolic content was 46.6 mg/g in autoclaved extract while the highest flavonoid content was 23.0 mg/g in chloroform extract. All extracts possessed tyrosinase inhibition activity and the inhibition activity was concentration-dependent. Inhibition concentration ($IC_{50}$) against tyrosinase activity was $0.36{\times}10^4$ mg/ml in ethanol extract, $0.11{\times}10^3$ mg/ml in acetone extract and 0.27 mg/ml in n-butanol extract. Among extracts tested, hot water and autoclaved extracts displayed higher antioxidative activity than organic solvent extracts. Therefore, our data suggest that extract from S. clava tunic may potential candidate for cosmetic product with whitening effect and medicine for diseases caused by various oxidative stresses.

Keywords

Antioxidant;Solvent extract;Styela clava;Whitening effect

Acknowledgement

Supported by : 농림수산식품부

References

  1. Ahn, S. H., Jung, S. H., Kang, S. J., Jeong, T. S., Choi, B. D., 2003, Extraction glycosaminoglycans from Styela clava tunic, Kor. J. Biotechnol. Bioeng., 18, 180-185.
  2. Amador, M. L., Jimeno, J., Paz-Ares, L., Cortes-Funes, H., Hidalgo, M., 2003, Progress in the development and acquisition of anticancer agents from marine sources. Ann. Oncol., 14, 1607-1615. https://doi.org/10.1093/annonc/mdg443
  3. An, S. H., 2004, Fractionation of glycosaminoglycans extracted from the Styela clava Tunic, MS Dissertation, Gyungsang National University, Jinju.
  4. Azzi, A., Davis, K. J. A., Kelly, F., 2004, Free radical biology-terminology and critical thinking, FEBS Lett., 558, 3-6. https://doi.org/10.1016/S0014-5793(03)01526-6
  5. Blois, M. S., 1958, Antioxidant determinations by the use of a stable free radical, Nature, 181, 1199-1200. https://doi.org/10.1038/1811199a0
  6. Cha, Y. J., Lee, H. Y., Jeong, E. J., 2008, Changes of biological activities of two ascidians, Styela calava and Styela plicata by cooking, J. Life Sci., 18, 109-113. https://doi.org/10.5352/JLS.2008.18.1.109
  7. Cho, Y. J., 2011, Characteristics of cosmetic with whitening compounds from Phellodendron amurense, J. Appl. Biol. Chem., 54, 108-113. https://doi.org/10.3839/jabc.2011.019
  8. Dinis, T. C. P., Madeira, V. M. C., Almeida, L. M., 1994, Action of phenolic derivatives (acetaminophen, salicylate, and 5-aminosalicylate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers, Arch. Biochem. Biophys., 315, 161-169. https://doi.org/10.1006/abbi.1994.1485
  9. Garcia-Fernandez, L. F., Fernando, R., Sanchex-Puelles, J. M., 2002, The marine pharmacy: New antitumoral compounds from the sea. Pharmaceutical News, 9, 495-501.
  10. Jo, Y. G., 1978, The sterol composition of Styela clava, Kor. Fish Soc., 11, 97-101.
  11. Jung, E. S., Park, E., Lee, S. C., 2008, Antioxidant activities of extracts from parts of Styela clava, J. Kor. Soc. Food Sci. Nutr., 37, 1674-1678. https://doi.org/10.3746/jkfn.2008.37.12.1674
  12. Jung, S W., Lee, N K., Kim, S J., Han, D. S., 1995, Screening of tyrosinase inhibitor from plants, Kor. J. Food Sci. Technol., 27, 891-896.
  13. Kim, J. J., Kim, S. J., Kim, S. H., Park, H. R., Lee, S. C., 2006, Antioxidant and anticancer activities of extracts from Styela clava according to the processing methods and solvents, J. Kor. Soc. Food Nutr., 35, 278-283. https://doi.org/10.3746/jkfn.2006.35.3.278
  14. Kim, Y. R., Ahn, S. H., Choi, B. D., Kang, S. J., Shin, G. W., Oh, M. J., Jung, T. S., 2004, In vitro examination of chondroitin sulfates extracted Midduck (Styela clava) and Munggae tunics (Halocynthia roretzi) as cosmetic material, J. Kor. Soc. Food Sci. Nutr., 33, 646-652. https://doi.org/10.3746/jkfn.2004.33.4.646
  15. Lee, J. J., Kim, A. R., Seo, Y. N., Lee, M. Y., 2009, Comparison of physicochemical composition of three species of genus Angelica, Kor. J Food Preserv., 16, 94-100.
  16. Lee, K. H, Park, C. S., Hong, B. I., Jung, B. C., Cho, H. S., Jea, Y. G., 1995, Seasonal variations of nutrients in warty sea squirt (Styela clava). J. Kor. Soc. Food Nutr., 24, 268-273.
  17. Lee, S. C., Kim, J. J., Kim, S. J., Kim, S. H., Park, H. R., 2006, Antioxidant and anticancer activities of extracts from Styela clava according to the processing method and solvents, J. Kor. Soc. Food Sci. Nutr., 35, 278-283. https://doi.org/10.3746/jkfn.2006.35.3.278
  18. Lim, N. Y., Kwon, G. J., Kim, Y. S., Baik, S. K., Lim, J. R., Mun, Y. j., Woo W. H., 2004, Inhibitory effect of methanolic extract from Adenophorae radix on melenogenesis, Kor. Ori. Physiol. Pathol. 18, 747-753.
  19. Marcocci, L., Maguire, J. J., Droylefaix, M. T., Packer, L., 1994, The nitric oxide-scavenging properties of Ginkgo biloba extract EGb 761, Biochem. Biophys. Res. Commun., 201, 748-755. https://doi.org/10.1006/bbrc.1994.1764
  20. Marklund, S., Marklund, G., 1974, Involvement of superoxide anion radical in the oxidation of pyrogallol and a convenient assay for superoxide dismutase, Eur. J. Biochem., 47, 469-474. https://doi.org/10.1111/j.1432-1033.1974.tb03714.x
  21. Nam, S. H., Choi, H. J., Hwang, S. M., Choi, Y. W., Lee, Y. G., Joo, W. H., 2013, Antimicrobial and anti-oral malodor efficacy of Schizandra chinensis extracts against oral pathogens, J. Life Sci., 23, 443-447. https://doi.org/10.5352/JLS.2013.23.3.443
  22. Oyaizu, M., 1986, Studies on products of the browning reaction. Antioxidative activities of browning reaction products prepared from glucosamine, Jpn. J. Nutr., 44, 307-315. https://doi.org/10.5264/eiyogakuzashi.44.307
  23. Park, S. M., Lee, B. B., Hwang, Y. M., Lee S. C., 2006, Quality properties of fish paste containing Styela clava, J. Kor. Soc. Food Sci. Nutr., 35, 908-911. https://doi.org/10.3746/jkfn.2006.35.7.908
  24. Prota, G., 1980, Recent advances in the chemistry of melanogenesis in mammals, J. Invest. Dermatol., 75, 122-127. https://doi.org/10.1111/1523-1747.ep12521344
  25. Singleton, V. L., Rossi, J. A., 1965, Colorimetry of total phenolics with phosphomolybdic- phosphotungstic acid reagents, Am. J. Enol. Viti., 16, 144-158.
  26. Woo, Y. M., Kim, A. J., Kim, J., Lee, C. H., 2011, Tyrosinase inhibitory compounds isolated from Persicaria tinctoria flower, J. Appl. Biol. Chem., 54, 47-50. https://doi.org/10.3839/jabc.2011.008
  27. Yagi, A., Kanbara, T., Morinobu, N., 1987, Inhibition of mushroom-tyrosinase by Aloe extract, Planta Med., 53, 515-517. https://doi.org/10.1055/s-2006-962798
  28. Zhishen, J., Mengcheng, T., Jianming, W., 1999, The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals, Food Chem., 64, 555-559. https://doi.org/10.1016/S0308-8146(98)00102-2

Cited by

  1. tunics in ICR mice vol.31, pp.3, 2015, https://doi.org/10.5625/lar.2015.31.3.125
  2. Protective effects of the antioxidant extract collected from Styela clava tunics on UV radiation-induced skin aging in hairless mice vol.38, pp.5, 2016, https://doi.org/10.3892/ijmm.2016.2740