Screening of Biological Activity of Solvent Extract from Styela clava Tunic for Fishery Waste Recycling

수산폐기물 재활용을 위한 미더덕껍질 용매 추출물의 생리활성 탐색

  • Lee, Sang-Mee (College of Natural Resources & Life Science, Pusan National University) ;
  • Kang, Eun-Jin (College of Natural Resources & Life Science, Pusan National University) ;
  • Go, Tae-Hun (College of Natural Resources & Life Science, Pusan National University) ;
  • Jeong, Seong-Yun (Department of Medical Life Science, Catholic University of Daegu) ;
  • Park, Geun-Tae (Research and University-Industry Cooperation, Pusan National University) ;
  • Lee, Hee-Sup (College of Human Ecology, Pusan National University) ;
  • Hwang, Dae-Youn (College of Natural Resources & Life Science, Pusan National University) ;
  • Jung, Young-Jin (College of Natural Resources & Life Science, Pusan National University) ;
  • Son, Hong-Joo (College of Natural Resources & Life Science, Pusan National University)
  • 이상미 (부산대학교 생명자원과학대학) ;
  • 강은진 (부산대학교 생명자원과학대학) ;
  • 고태훈 (부산대학교 생명자원과학대학) ;
  • 정성윤 (대구가톨릭대 의생명과학과) ;
  • 박근태 (부산대학교 산학협력단) ;
  • 이희섭 (부산대학교 생활환경대학) ;
  • 황대연 (부산대학교 생명자원과학대학) ;
  • 정영진 (부산대학교 생명자원과학대학) ;
  • 손홍주 (부산대학교 생명자원과학대학)
  • Received : 2013.07.02
  • Accepted : 2013.08.12
  • Published : 2014.01.31


Styela clava tunic is generated in large amounts as a waste from S. clava processing plants and causes environmental problem. Although biological activities of S. clava were reported by many investigators, study on S. clava tunic was little. In this study, therefore, tyrosinase inhibition and antioxidative activities of extracts from S. clava tunic using different solvent were investigated for recycling of the fishery waste. Among extraction methods tested, autoclaved extraction (25.7%) and hot water extraction (18.2%) appeared to be effective for extraction. The highest total phenolic content was 46.6 mg/g in autoclaved extract while the highest flavonoid content was 23.0 mg/g in chloroform extract. All extracts possessed tyrosinase inhibition activity and the inhibition activity was concentration-dependent. Inhibition concentration ($IC_{50}$) against tyrosinase activity was $0.36{\times}10^4$ mg/ml in ethanol extract, $0.11{\times}10^3$ mg/ml in acetone extract and 0.27 mg/ml in n-butanol extract. Among extracts tested, hot water and autoclaved extracts displayed higher antioxidative activity than organic solvent extracts. Therefore, our data suggest that extract from S. clava tunic may potential candidate for cosmetic product with whitening effect and medicine for diseases caused by various oxidative stresses.


Antioxidant;Solvent extract;Styela clava;Whitening effect


Supported by : 농림수산식품부


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