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Molecular Characterization and Expression Analysis of a Glutathione S-Transferase cDNA from Abalone (Haliotis discus hannai)

북방전복 (Haliotis discus hannai)에서 분리한 Glutathione S-transferase 유전자의 분자생물학적 고찰 및 발현분석

  • 문지영 (국립수산과학원 전략양식연구소 생명공학과) ;
  • 박은희 (국립수산과학원 전략양식연구소 생명공학과) ;
  • 공희정 (국립수산과학원 전략양식연구소 생명공학과) ;
  • 김동균 (국립수산과학원 전략양식연구소 생명공학과) ;
  • 김영옥 (국립수산과학원 전략양식연구소 생명공학과) ;
  • 김우진 (국립수산과학원 전략양식연구소 생명공학과) ;
  • 안철민 (국립수산과학원 전략양식연구소 생명공학과) ;
  • 남보혜 (국립수산과학원 전략양식연구소 생명공학과)
  • Received : 2014.12.01
  • Accepted : 2014.12.24
  • Published : 2014.12.31

Abstract

Glutathione S-transferases (GSTs) are a superfamily of detoxification enzymes that primarily catalyze the nucleophilic addition of reduced glutathione to both endogenous and exogenous electrophiles. In this study, we isolated and characterized a full-length of alpha class GST cDNA from the abalone (Haliotis discus hannai). The abalone GST cDNA encodes a 223-amino acid polypeptide with a calculated molecular mass of 25.8 kDa and isoelectric point of 5.69. Multiple alignments and phylogenetic analysis with the deduced abalone GST protein revealed that it belongs to the alpha class GSTs and showed strong homology with disk abalone (Haliotis discus discus) putative alpha class GST. Abalone GST mRNA was ubiquitously detected in all tested tissues. GST mRNA expression was comparatively high in the mantle, gill, liver, and digestive duct, however, lowest in the hemocytes. Expression level of abalone GST mRNA in the mantle, gill, liver, and digestive duct was 182.7-fold, 114.8-fold, 4675.8-fold, 406.1-fold higher than in the hemocytes, respectively. Expression level of abalone GST mRNA in the liver was peaked at 6 h post-infection with Vibrio parahemolyticus and decreased at 12 h post-infection. While the expression level of abalone GST mRNA in the hemocytes was drastically increased at 3 h post-infection with Vibrio parahemolyticus. These results suggest that abalone GST is conserved through evolution and may play roles similar to its mammalian counterparts.

Acknowledgement

Supported by : 국립수산과학원

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