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Overwintering Conditions of the Diamondback Moth and Genetic Variation of Overwintering Populations

배추좀나방 월동 조건과 야외 월동집단의 유전적 변이

  • Kim, Eunseong (Department of Bioresource Sciences, Andong National University) ;
  • Choi, Bongki (Department of Applied Biology, Kyungpook National University) ;
  • Park, Youngjin (Department of Bioresource Sciences, Andong National University) ;
  • Cha, Ookhyun (Department of Biology, Kyungsung University) ;
  • Jung, Chungryel (Department of Biology, Kyungsung University) ;
  • Lee, Daeweon (Department of Biology, Kyungsung University) ;
  • Kim, Kwangho (Crop Protection Division, National Academy of Agriculture Science) ;
  • Kim, Yonggyun (Department of Bioresource Sciences, Andong National University)
  • 김은성 (안동대학교 자연과학대학 생명자원과학과) ;
  • 최봉기 (경북대학교 농업생명과학대학 응용생명과학과) ;
  • 박영진 (안동대학교 자연과학대학 생명자원과학과) ;
  • 차욱현 (경성대학교 자연과학대학 생물학과) ;
  • 정충렬 (경성대학교 자연과학대학 생물학과) ;
  • 이대원 (경성대학교 자연과학대학 생물학과) ;
  • 김광호 (국립농업과학원 작물보호과) ;
  • 김용균 (안동대학교 자연과학대학 생명자원과학과)
  • Received : 2014.06.17
  • Accepted : 2014.10.02
  • Published : 2014.12.01

Abstract

It has been unclear whether the diamondback moth, Plutella xylostella can overwinter in Korean field conditions. This study determined overwintering conditions of P. xylostella by conducting field exposure tests based on its cold tolerance and monitoring overwintering populations by direct examination of overwintering larval habitats and capturing adults with sex pheromone traps. In addition, the overwintering populations were analyzed using polymorphic genetic markers to trace their sources. When all immature stages of P. xylostella were exposed to $-5^{\circ}C$, which was the temperature much above their supercooling points, they significantly suffered with direct cold injuries, where larval stage was most tolerant to the cold injury. However, the exposure to $5^{\circ}C$ for a long period (4 weeks) did not give any significant cold injury to nonfeeding stages, while this treatment gave lethality to larval stage without diet. When all developmental stages of P. xylostella were exposed to open field conditions during winter, they exhibited significant decreases of survival rates. However, some protected and indoor conditions reduced the cold injuries and the diet provision significantly increased larval survival rates. Adult monitoring with sex pheromone during winter period indicated that the first captures were observed at similar periods at different locations (${\approx}260$ Km apart). The overwintering adults were captured until early April. Genetic variation of these overwintering populations was analyzed with polymorphic molecular markers, indicating significant genetic divergences among the overwintering populations. This study indicates that P. xylostella can overwinter in southern Korean fields or some protected greenhouses with host plants.

Acknowledgement

Supported by : 농촌진흥청

References

  1. Bale, J.S., 1987. Insect cold hardiness: freezing and supercooling-an ecophysiological perspective. J. Insect Physiol. 33, 899-908. https://doi.org/10.1016/0022-1910(87)90001-1
  2. Caprio, M.A., Tabashnik, B., 1992. Allozymes used to estimate gene flow among populations of diamondback moth (Lepidoptera: Plutellidae) in Hawaii. Environ. Entomol. 21, 808-816. https://doi.org/10.1093/ee/21.4.808
  3. Chen, C.P., Denlinger, D.L., Lee, R.E., Jr., 1987. Cold shock injury and rapid cold hardening in the flesh fly, Sarcophaga crassipalpis. Physiol. Zool. 60, 297-304.
  4. Choi, B., Hepat, R., Kim, Y., 2014. RNA interference of a heat shock protein, Hsp70, loses its protection role in indirect chilling injury to the beet armyworm, Spodoptera exigua. Comp. Biochem. Physiol. A 168, 90-95.
  5. Danks, H.V., 2002. Modification of adverse conditions by insects. Oikos 99, 10-24. https://doi.org/10.1034/j.1600-0706.2002.990102.x
  6. Denlinger, D.L., 2002. Regulation of diapause. Annu. Rev. Entomol. 47, 93-122.
  7. Duman, J.G., 2001. Antifreeze and ice nucleator proteins in terrestrial arthropods. Annu. Rev. Physiol. 63, 327-357. https://doi.org/10.1146/annurev.physiol.63.1.327
  8. Hayakawa, H., Tsutsui, H., Goto, C., 1988. A survey of overwintering of the diamondback moth, Plutella xylostella Linne, in the Tokachi district of Hokkaido. Ann. Rept. Plant Prot. North Japan 39, 227-228.
  9. Honda, K.I., 1992. Hibernation and migration of diamondbackmoth in northern Japan. pp. 43-50, in Management of diamondbackmoth and other crucifer pests: proceedings of the second internationalworkshop. Ed. N.S. Talekar. Asian Vegetable Researchand Development Center. Shanhua, Taiwan.
  10. Hong, S.T., Bang, S., Hyun, S., Kang, J., Jeong, K., Paik, D., Chung, J., Kim, J., 2008. cAMP signalling in mushroom bodies modulates temperature preference behavior in Drosophila. Nature 454, 771-775.
  11. Kang, J., Kim, J., Choi, K.W., 2011. Novel cytochrome P450, cyp6a17, is required for temperature preference behavior in Drosophila. PLoS One 6, e29800. https://doi.org/10.1371/journal.pone.0029800
  12. Kim, I., Bae, J.S., Choi, K.H., Jin, B.R., Lee, K.R., Sohn, H.D., 2000. Haplotype diversity and gene flow of the diamondback moth, Plutella xylostella (L.) (Lepidoptera: Yponomeutidae), in Korea. Kor. J. Appl. Entomol. 39, 43-52.
  13. Kim, M., Lee, S.C., 1991. Bionomics of diamondback moth, Plutella xylostella (Lepidoptera: Plutellidae) in southern region of Korea. Kor. J. Appl. Entomol. 30, 169-173.
  14. Kim, Y., Bae, S., Son, Y., Park, J., 2009. Analysis of migration of the oriental fruit moth, Grapholita molesta, in apple-cultivating areas based on population monitoring using sex pheromone and RAPD molecular marker. Kor. J. Appl. Entomol. 48, 211-219. https://doi.org/10.5656/KSAE.2009.48.2.211
  15. Kim, Y., Kim, K., Kim, N., 1999. Genetic difference of two field populations of Plutella xylostella (Linne) (Lepidoptera: Yponomeutidae) measured with four polymorphic allozymes. J. Asia Pac. Entomol. 2, 1-5. https://doi.org/10.1016/S1226-8615(08)60024-2
  16. Kim, Y., Park, H., Chung, M., 2001. Genetic analysis of overwinteringdiamondback moth, Plutella xylostella (Linne), populationsusing four polymorphic allozymes. Kor. J. Appl. Entomol.40, 227-233.
  17. Kim, Y.H., Lee, J.H., Lee, S.H., 2011. Determination of organophosphate and carbamate resistance allele frequency in diamondback moth populations by quantitative sequencing and inhibition tests. J. Asia Pac. Entomol. 14, 29-33. https://doi.org/10.1016/j.aspen.2010.11.007
  18. Kwon, D.H., Choi, B.R., Park, H.M., Lee, S.H., Miyata, T., Clark, J.M., Lee, S.H., 2004. Knockdown resistance allele frequency in field populations of Plutella xylostella in Korea. Pestic. Biochem. Physiol. 80, 21-30
  19. Lee, R.E., Jr., 1989. Insect cold-hardiness: to freeze or not to freeze. BioScience 39, 308-313. https://doi.org/10.2307/1311113
  20. Lee, R.E., Jr., Damodaran, K., Yi, S.X., Lorigan, G.A., 2006. Rapid cold-hardening increases membrane fluidity and cold tolerance of insect cells. Cryobiology 52, 459-463. https://doi.org/10.1016/j.cryobiol.2006.03.003
  21. Lee, R.E., Jr., Denlinger, D.L., 1991. Insects at low temperatures. Chapman and Hall, New York.
  22. Neely, G.G., Keene, A.C., Duchek, P., Chang, E.C., Wang, Q.P., Aksoy, Y.A., Rosenzweig, M., Costigan, M., Woolf, C.J., Garrity, P.A., Penninger, J.M., 2011. TrpA1 regulates thermal nociception in Drosophila. PLoS One 6, e24343. https://doi.org/10.1371/journal.pone.0024343
  23. Nei, M., Maruyama, T., Chakraborty, R., 1975. The bottleneck effect and genetic variability in populations. Evolution 29, 1-10. https://doi.org/10.2307/2407137
  24. Nei, M., 1978. Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89, 583-590
  25. Park, Y., Kim, Y., 2014. A specific glycerol kinase induces rapid cold hardening of the diamondback moth, Plutella xylostella. J. Insect Physiol. 67, 56-63.
  26. Pfister, T.D., Storey, K.B., 2006. Insect freeze tolerance: roles of protein phosphatases and protein kinase A. Insect Biochem. Mol. Biol. 36, 18-24.
  27. Philip, B.N., Lee, R.E., Jr., 2010. Changes in abundance of aquaporin- like proteins occurs concomitantly with seasonal acquisition of freeze tolerance in the goldenrod gall fly, Eurosta solidaginis. J. Insect Physiol. 56, 679-685. https://doi.org/10.1016/j.jinsphys.2009.12.003
  28. Romlov, H., Lee, R.E., Jr., 2000. Extreme resistance to desiccation in overwintering larvae of the gall fly Eurosta solidaginis (Diptera, Tephritidae). J. Exp. Biol. 203, 783-789.
  29. Rozsypal, J., Kostal, V., Zahradnickova, H., Simek, P., 2013. Overwintering strategy and mechanisms of cold tolerance in the coding moth (Cydia pomonella). PLoS One 8, e61745. https://doi.org/10.1371/journal.pone.0061745
  30. SAS Institute, Inc., 1989. SAS/STAT User's Guide, Release 6.03, Ed. Cary, NC, USA.
  31. Storey, K.B., Storey, J.M., 1996. Natural freezing survival in animals. Annu. Rev. Ecol. Syst. 27, 365-386. https://doi.org/10.1146/annurev.ecolsys.27.1.365
  32. Storey, K.B., Storey, J.M., 1998. Freeze tolerance in animals. Physiol. Rev. 68, 27-84.
  33. Storey, K.B., Storey, J.M., 2012. Insect cold hardiness: metabolic, gene, and protein adaptation. Can. J. Zool. 90, 456-475.
  34. Tabashnik, B.E., Liu, Y.B., Malvar, T., Heckel, D.G., Masson, L., Ballester, V., Granero, F., Mensua, J.L., Ferre, J., 1997. Global variation in the genetic and biochemical basis of diamondback moth resistance to Bacillus thuringiensis. Proc. Natl. Acad. Sci. USA 94, 12780-12785.
  35. Wright, S., 1951. The genetical structure of populations. Annu. Eugen. 15, 323-354.
  36. Yuan, G., Gao, W., Yang, Y., Wu, Y., 2010. Molecular cloning, genomic structure, and genetic mapping of two RDL-orthologous genes of GABA receptors in the diamondback moth, Plutella xylostella. Arch. Insect Biochem. Physiol. 74, 81-90.

Cited by

  1. Decrease in Genetic Variation of Overwintering Populations of the Diamondback Moth during Seasonal Occurrence 2015, https://doi.org/10.5656/KSAE.2015.08.0.028
  2. Differential Susceptibility to High Temperature and Variation of Seasonal Occurrence between Spodoptera exigua and Plutella xylostella 2016, https://doi.org/10.5656/KSAE.2016.01.1.074
  3. Elusively overwintering: a review of diamondback moth (Lepidoptera: Plutellidae) cold tolerance and overwintering strategy vol.150, pp.02, 2018, https://doi.org/10.4039/tce.2018.2