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Zinc Restored the Decreased Vascular Smooth Muscle Cell Viability under Atherosclerotic Calcification Conditions

  • Shin, Mee-Young (Department of Food Science and Nutrition, College of Human Ecology, Andong National University) ;
  • Kwun, In-Sook (Department of Food Science and Nutrition, College of Human Ecology, Andong National University)
  • Received : 2014.12.01
  • Accepted : 2014.12.07
  • Published : 2014.12.31

Abstract

Zinc is considered to be involved in maintaining healthy vascular condition. Atherosclerotic calcification of vascular smooth muscle cells (VSMCs) occurs via the mechanism of cell death; therefore, cell viability is a critical factor for preventing VSMC calcification. In this study, we tested whether zinc affected VSMC viability under both normal physiological non-calcifying (0 mM P) and atherosclerotic calcifying conditions (3 and 5 mM P), since VSMC physiological characters change during the VSMC calcification process. The study results showed that an optimal zinc level ($15{\mu}M$) restored the decreased VSMC viability which was induced under low zinc levels (0 and $1{\mu}M$) and calcifying conditions (3 and 5 mM P) at 9 and 15 days culture. This zinc-protecting effect for VSMC viability is more prominent under atherosclerotic calcifying condition (3 and 5 mM P) than normal condition (0 mM P). Also, the increased VSMC viability was consistent with the decreased Ca and P accumulation in VSMC cell layers. The results suggested that zinc could be an effective biomineral for preventing VSMC calcification under atherosclerotic calcifying conditions.

Acknowledgement

Supported by : National Research Foundation of Korea

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Cited by

  1. Nutrition and Atherosclerosis vol.46, pp.5, 2015, https://doi.org/10.1016/j.arcmed.2015.05.010