DOI QR코드

DOI QR Code

Establishment of proliferation and regeneration system of PLBs in Phalaenopsis by treatments of a variety of types of medium, sucrose concentrations and anti-browning agents

다양한 배지종류, sucrose 농도 및 갈변억제물질 처리에 의한 팔레놉시스 PLB 증식 및 재분화 체계확립

  • Received : 2014.11.14
  • Accepted : 2014.11.18
  • Published : 2014.12.31

Abstract

To establish an efficient proliferation and regeneration of PLBs (protocorm-like bodies) of Phalaenopsis plants, a variety of propagation medium types, various concentraions of sucrose as well as liquid and solid type were tested in this study. Further, activated charcoal, citric acid and ascorbic acid were compared whether these agents are suppose to reduce the browning in culture process using PLBs of Phalanopsis plants. With regard to the proper propagation medium, VW medium showed 1.3 ~ 2 times highr than those of other medium in an index of increasing for fresh weight and 50% higher than those of other medium in the frequency of shoot regeneration. However, regarding liquid and solid types of culture, there were no significant differences in the proliferation of PLBs and regeneration of shoots from PLBs. In the experiment for a variety of sucrose concentrations (0 ~ 50 g/l), 10 g of sucrose showed 30 ~ 50% higher than other concentrations in increasing index and 10 ~ 50% higher in the regeneration of shoots from PLBs. Regarding the reduction of browning in tissue culture via PLBs of Phalaenopsis plants, 1 g of activated charcoal showed only 1.5% browning of PLBs cultured. Whereas, other treatments including citric acid and ascorbic acid showed 6 ~ 16% of browning of PLBs. Therefore, activated charcoal was selected as an efficient anti-browning agents for the culture of PLBs in Phalaenopsis plants. Using above-described results can be contibuted to the establishment of mass propagation system using PLBs of Phalaenopsis plants in the future.

Keywords

Orchids;Phalaenopsis;PLBs (protocorm-like bodies);Regeneration;Tissue culture

References

  1. Abdelwahd R, Hakam N, Labhilili M, Udupa SM (2008) Use of an adsorbent and antioxidants to reduce the effects of leached phenolics in in vitro plantlet regeneration of faba bean. Afri J of Biotech 7(8):997-1002
  2. Anthony JM, Senaratna T, Dixon KW, Sivasithamparam K (2004) The role of antioxidants for initiation of somatic embryos with Conostephium pendulum (Ericaceae). Plant Cell Tiss and Org Cult 78(3):247-252 https://doi.org/10.1023/B:TICU.0000025661.56250.b4
  3. Been CG (2003) Production of Phalaenopsis clones by shoot propagation. Flower Res J 11(2):171-176
  4. Carlberg I, Glimelius K, Eriksson T (1983) Improved culture ability of potato protoplasts by use of activated charcoal. Plant Cell Rep 2:223-225 https://doi.org/10.1007/BF00269145
  5. Eum S.J, Park KI, Oh W, Kim KW (2010) Promotion of Bulblet Enlargement through Liquid Stationary Culture in Korean Native Lilies in Vitro. Hort Environ biotechnol 51(1):45-50
  6. Homma Y and T Asahira (1985) New means of Phalaenopsis propagation with internodal zawa sections of flower stalk. J of the Jpn Soc for Hort Sci 54:379-387 https://doi.org/10.2503/jjshs.54.379
  7. Inchihashi S (1992) Micropropagation of Phalaenopsis through the culture of lateral buds from young flower stalks. Lindleyana 7:208-215
  8. Inchihashi S (1993) Phalaenopsis (Breeding and culture) III carbohydrate source on growth of embryogenic callus in Phalaenopsis and allied genera. Hirosawa Univ 10:81-88
  9. Intuwong O and Y sagawa (1974) Clonal propagation of Phalaenopsis by shoot tip culture. Ameri Orchid Soc Bull 43:893-895
  10. Karami O, Deljou A, Esna-Ashari M, Ostad-Ahmadi P (2006) Effect of sucrose concentrations on somatic embryogenesis in carnation (Dianthus caryophyllus L.). Sci Hort 110(4):340-344 https://doi.org/10.1016/j.scienta.2006.07.029
  11. Kauth PJ, Dutra D, Johnson TR, Stewart SL, Kane ME, Vendrame W (2008) Techniques and applications of in vitro orchid seed germination. Floriculture, ornamental and plant biotechnology: advances and topical issues 5:375-391
  12. Kim JH (2010) Production system of in vitro Phalaenopsis plants and problems on domestic tissue culture facilities in Korea. 10th Orchid symposium. Abstract p. 11-19
  13. Kim MS, Eun JS, Kim JY (2001b) Effect of culture medium, temperature, and light intensity on PLB propagation of Phalaenopsis. Kor J Plant Tissue culture 28(4):215-219
  14. Kim WJ, Jeong HY, Kim YJ, Hwang KS, Choi SR, Shin HK, Kim HD, Koo DH, Cho HR, Lee HK, Lim JH, Kim KJ, Kim JY, Lee DW, Song JS, Lee KS, Yoo BS, Kim WH, Oh YN, Lee EK, Jeong MI, Kim MS, Bang CS, Lee YR, Yoo EH, Lee YS (2001a) Breeding techniques in ornamentals, RDA, Suwon, p. 289-309
  15. Knudson L (1946) A new nutrient solution for germination of orchid seed. Am Orchid Soc Bull 15:2124-217
  16. Ko WH, Su, CC, Chen CL, Chao CP (2009) Control of lethal browning of tissue culture plantlets of Cavendish banana cv. Formosana with ascorbic acid. Plant Cell Tiss Org Cult 96(2):137-141 https://doi.org/10.1007/s11240-008-9469-7
  17. Kobayashi M, Komatuda M, Yonar S (1991) Studies on the vegetative propagation of Phalaenopsis through root tip culture Abstr. J of Jpn Soc for Hort Sci 59:664-665
  18. Kusumoto M (1980) Effects of coconut milk, agar and sucrose concentrations and medium pH on the proliferation of Cymbidium protocorm-like bodies cultured in vitro. J of the Jap Soc for Hort Sci 48(4):503-509 https://doi.org/10.2503/jjshs.48.503
  19. Lee YM, Kim MS, Lee SI, Kim JB (2010) Review on breeding, tissue culture and genetic transformation systems in Cymbidium. J Plant Biotechnol 37:357-365 https://doi.org/10.5010/JPB.2010.37.4.357
  20. Lin CC (1986) In vitro culture of flower stalk internodes of Phalaenopsis and Dorutaenopsis. Lindleyan
  21. Madhusudhanan K and Rahiman BA (2000) The effect of activated charcoal supplemented media to browning of in vitro culture of Piper species. Biologya Plant 143(2):297-299
  22. Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15(3):473-497 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  23. Paek KY, (2001) Plant tissue culture techniques, Hyang-Moon publisher, Seoul, p. 466
  24. Paek KY, Hahn EJ, Park SY (2011) Micropropagation of Phalaenopsis orchids via protocorms and protocorm-like bodies. Methods in molecular biology (Clifton, NJ), 710:293-306 https://doi.org/10.1007/978-1-61737-988-8_20
  25. Park YS, KakutaS, Kano A, Okabe M (1996) Effcient propagation of protocorm-like bodies of Phalaenopsis in liquid medium. Plant Cell Tiss Org Cult 45:79-85 https://doi.org/10.1007/BF00043432
  26. Roter G (1949) A method of vegetative propagation of Phalaenopsis species and hybrids. A O S Bull 18:738-739
  27. Roubelakis-Angelakis KA, Zivanovitc SB (1991) A new culture medium for in vitro rhizogenesis of grapevine (Vitis spp.) genotypes. HortScience 26(12):1551-1553
  28. Tanaka M and Y sakanishi (1978) Factors affecting the growth of in vitro cultured lateral bods from Phalaenopsis flower stalks. Sci Hort 8:169-173 https://doi.org/10.1016/0304-4238(78)90022-5
  29. Teixeira da Silva JA, Chan MT, Chai ML, Tanaka M (2006) Priming abiotic factors for optimal hybrid Cymbidium (Orchidaceae) PLB and callus induction, plantlet formation, and their subsequent cytogenetic stability analysis. Sci Hort 109(4):368-378 https://doi.org/10.1016/j.scienta.2006.05.016
  30. Teixeirada silva JA (2013) Impact of paper bridges, activated charcoal, and antoxidants on growthand development of protocorm-like bodies of hybrid Cymbidium. In Vitro Cell Dev Biol Plant 49:414-420 https://doi.org/10.1007/s11627-013-9532-x
  31. Tokuhara K, Mill M (1993) Micropropagation of Phalaenopsis and Doritaenopsis by culturing shoot tips of flower stalk buds. Plant Cell Rep 13(1):7-11
  32. Tse AT, Smith RJ, Hackett WP (1971) Adventitious shoot formation on Phalaenopsis nodes. Ameri Orchid Soc Bull 40:807-810
  33. Vacin E and Went FW (1949) Some pH changes in nutrient solutions. Bot Gaz 110:605-613 https://doi.org/10.1086/335561
  34. Yun KY and Jeong SY (2011) Orchids of World. Kim-Young publisher, Pa-ju, Kyong-Ki, Korea

Acknowledgement

Supported by : 농촌진흥청