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A Piezoelectric Immunosensor for Early Cervical Cancer Detection

  • Yang, Li (Frequency Control Laboratory, School of Automation and Engineering, University of Electronic Science and Technology of China) ;
  • Huang, Xianhe (Frequency Control Laboratory, School of Automation and Engineering, University of Electronic Science and Technology of China) ;
  • Sun, Liang (Department of Pathology, West China Second University Hospital, Sichuan University)
  • Published : 2014.11.28

Abstract

Background: A piezoelectric immunosensor for early cervical cancer detection was developed involving short analyis time and less invasive technique for $p16^{INK4a}$, a protein that has been linked to cervical cancer. Materials and Methods: $5{\mu}L$ of 5.0 mg/mL $p16^{INK4a}$ antibody and then supernatant from different clinical samples from West China Second University Hospital (Sichuan, China) were dripped on the center of the AT-cut crystal through a micro-injector. Absorption of the $p16^{INK4a}$ by antibody caused a shift in the resonant frequency of the immunosensor, and the resonant frequency was correlated to the amount of the $p16^{INK4a}$ in the supernatant. Results: The greater severity of lesion grading, the greater the expression level of $p16^{INK4a}$. Conclusion: Degree of cervical cancer lesion development could be determined by detected amount of $p16^{INK4a}$ in different clinical samples.

Acknowledgement

Supported by : Southwest university

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