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Expression and Clinical Significance of miRNA-34a in Colorectal Cancer

  • Ma, Zhi-Bin (Department of Gastroenterology, the First Affiliated Hospital of Harbin Medical University) ;
  • Kong, Xiao-Lin (Department of Hematology, the First Affiliated Hospital of Harbin Medical University) ;
  • Cui, Gang (Department of Hematology, the First Affiliated Hospital of Harbin Medical University) ;
  • Ren, Cui-Cui (Department of Hematology, the First Affiliated Hospital of Harbin Medical University) ;
  • Zhang, Ying-Jie (Department of Hematology, the First Affiliated Hospital of Harbin Medical University) ;
  • Fan, Sheng-Jin (Department of Hematology, the First Affiliated Hospital of Harbin Medical University) ;
  • Li, Ying-Hua (Department of Hematology, the First Affiliated Hospital of Harbin Medical University)
  • Published : 2014.11.28

Abstract

Background: The aim of this study was to investigate differences of miRNA-34a expression in benign and malignant colorectal lesions. Materials and Methods: Samples of cancer, paraneoplastic tissues and polyps were selected and total RNA was extracted by conventional methods for real-time PCR to detect the miRNA-34a expression. In addition, the LOVO colorectal cancer cell line was cultured, treated with the demethylating agent 5-azacytidine and screened for differentially expressed miRNA-34a. Results: After the drug treatment, the miRNA-34a expression of colorectal cancer cell line LOVO was increased and real-time PCR showed that levels of expression in both cell line and colorectal cancer tissues were low, as compared to paraneoplastic tissue (p<0.05). Polyps tissues had significantly higher expression than paraneoplastic and colorectal cancer samples (p<0.05). Conclusions: miRNA-34a-5p may play a role as a tumor suppressor gene in colorectal cancer, with involvement of DNA methylation.

Acknowledgement

Supported by : Department of Education in Heilongjiang Province

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