DOI QR코드

DOI QR Code

miR-205 in Situ Expression and Localization in Head and Neck Tumors - a Tissue Array Study

  • Ab Mutalib, Nurul-Syakima (Department of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia) ;
  • Lee, Learn-Han (Department of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia) ;
  • Cheah, Yoke-Kqueen (Department of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia)
  • Published : 2014.11.28

Abstract

Background: microRNAs are small non-coding RNA that control gene expression by mRNA degradation or translational inhibition. These molecules are known to play essential roles in many biological and physiological processes. miR-205 may be differentially expressed in head and neck cancers; however, there are conflicting data and localization of expression has yet to be determined. Materials and Methods: miR-205 expression was investigated in 48 cases of inflammatory, benign and malignant tumor tissue array of the neck, oronasopharynx, larynx and salivary glands by Locked Nucleic Acid in situ hybridization (LNA-ISH) technology. Results: miR-205 expression was significantly differentially expressed across all of the inflammatory, benign and malignant tumor tissues of the neck. A significant increase in miR-205 staining intensity (p<0.05) was observed from inflammation to benign and malignant tumors in head and neck tissue array, suggesting that miR-205 could be a biomarker to differentiate between cancer and non-cancer tissues. Conclusions: LNA-ISH revealed that miR-205 exhibited significant differential cytoplasmic and nuclear staining among inflammation, benign and malignant tumors of head and neck. miR-205 was not only exclusively expressed in squamous epithelial malignancy. This study offers information and a basis for a comprehensive study of the role of miR-205 that may be useful as a biomarker and/or therapeutic target in head and neck tumors.

References

  1. Avissar M, Christensen BC, Kelsey KT, et al (2009). MicroRNA expression ratio is predictive of head and neck squamous cell carcinoma. Clin Cancer Res, 15, 2850-55. https://doi.org/10.1158/1078-0432.CCR-08-3131
  2. Babu JM, Prathibha R, Jijith VS, et al (2011). A miR-centric view of head and neck cancers. Biochim Biophys Acta, 1816, 67-72.
  3. Baranwal S, Alahari SK (2010). miRNA control of tumor cell invasion and metastasis. Int J Cancer, 126, 1283-90.
  4. Benes V, Castoldi M (2010). Expression profiling of microRNA using real-time quantitative PCR, how to use it and what is available. Methods, 50, 244-9. https://doi.org/10.1016/j.ymeth.2010.01.026
  5. Chen HC, Chen GH, Chen YH, et al (2009). MicroRNA deregulation and pathway alterations in nasopharyngeal carcinoma. Br J Cancer, 100, 1002-11. https://doi.org/10.1038/sj.bjc.6604948
  6. Childs G, Fazzari M, Kung G, et al (2009). Low-level expression of microRNAs let-7d and miR-205 are prognostic markers of head and neck squamous cell carcinoma. Am J Pathol, 174, 736-45. https://doi.org/10.2353/ajpath.2009.080731
  7. Darnell DK, Kaur S, Stanislaw S, et al (2006). MicroRNA expression during chick embryo development. Dev Dyn, 235, 3156-65. https://doi.org/10.1002/dvdy.20956
  8. Git A, Dvinge H, Salmon-Divon M, et al (2010). Systematic comparison of microarray profiling, real-time PCR, and next-generation sequencing technologies for measuring differential microRNA expression. RNA, 16, 991-1006. https://doi.org/10.1261/rna.1947110
  9. Harris T, Jimenez L, Kawachi N, et al (2012). Low-level expression of miR-375 correlates with poor outcome and metastasis while altering the invasive properties of head and neck squamous cell carcinomas. Am J Pathol, 180, 917-28. https://doi.org/10.1016/j.ajpath.2011.12.004
  10. Jemal A, Bray F, Center MM, et al (2011).Global cancer statistics. CA Cancer J Clin, 61, 69-90. https://doi.org/10.3322/caac.20107
  11. Jiang J, Lee EJ, Gusev Y, et al (2005). Real-time expression profiling of microRNA precursors in human cancer cell lines. Nucleic Acids Res, 33, 5394-403. https://doi.org/10.1093/nar/gki863
  12. Kimura S, Naganuma S, Susuki D, et al (2010). Expression of microRNAs in squamous cell carcinoma of human head and neck and the esophagus: miR-205 and miR-21 are specific markers for HNSCC and ESCC. Oncol Rep, 23, 1625-33.
  13. Kozomara A, Griffiths-Jones S (2014). miRBase: annotating high confidence microRNAs using deep sequencing data. Nucl Acids Res, 42, 68-73.
  14. Krutovskikh VA, Herceg Z (2010).Oncogenic microRNAs (OncomiRs) as a new class of cancer biomarkers. Bioessays, 32, 894-904. https://doi.org/10.1002/bies.201000040
  15. Lagos-Quintana M, Rauhut R, Lendeckel W, et al (2001). Identification of novel genes coding for small expressed RNAs. Science, 294, 853-8. https://doi.org/10.1126/science.1064921
  16. Lee RC, Ambros V (2001). An extensive class of small RNAs in Caenorhabditis elegans. Science, 294, 862-4. https://doi.org/10.1126/science.1065329
  17. Lei L, Huang Y, Gong W (2013). miR-205 promotes the growth, metastasis and chemoresistance of NSCLC cells by targeting PTEN. Oncol Rep, 30, 2897-902.
  18. Liu CJ, Shen WG, Peng SY, et al (2014). miR-134 induces oncogenicity and metastasis in head and neck carcinoma through targeting WWOX gene. Int J Cancer, 134, 811-21. https://doi.org/10.1002/ijc.28358
  19. Liu X, Yu J, Jiang L, et al (2009). MicroRNA-222 regulates cell invasion by targeting matrix metalloproteinase 1 (MMP1) and manganese superoxide dismutase 2 (SOD2) in tongue squamous cell carcinoma cell lines. Cancer Genomics Proteomics, 6, 131-9.
  20. Lu J, Luo H, Liu X, et al (2014). miR-9 targets CXCR4 and functions as a potential tumor suppressor in nasopharyngeal carcinoma. Carcinogenesis, (epub ahead of print).
  21. Macfarlane LA, Murphy PR (2010). MicroRNA: Biogenesis, Function and Role in Cancer. Curr Genomics, 11, 537-61. https://doi.org/10.2174/138920210793175895
  22. Mehanna H, Paleri V, West CM, et al (2010). Head and neck cancer--Part 1: Epidemiology, presentation, and prevention. BMJ, 341, 4684. https://doi.org/10.1136/bmj.c4684
  23. Nohata N, Hanazawa T, Kikkawa N, et al (2011a). Tumor suppressive microRNA-375 regulates oncogene AEG 1/MTDH in head and neck squamous cell carcinoma (HNSCC). J Hum Genet, 56, 595-601. https://doi.org/10.1038/jhg.2011.66
  24. Nohata N, Sone Y, Hanazawa T, et al (2011b). miR-1 as a tumor suppressive microRNA targeting TAGLN2 in head and neck squamous cell carcinoma. Oncotarget, 2, 29-42.
  25. Nuovo GJ (2008). In situ detection of precursor and mature microRNAs in paraffin embedded, formalin fixed tissues and cell preparations. Methods, 44, 39-46. https://doi.org/10.1016/j.ymeth.2007.10.008
  26. Nuovo GJ, Elton TS, Nana-Sinkam P, et al (2009). A methodology for the combined in situ analyses of the precursor and mature forms of microRNAs and correlation with their putative targets. Nat Protoc, 4, 107-15. https://doi.org/10.1038/nprot.2008.215
  27. Obernosterer G, Martinez J, Alenius M (2007). Locked nucleic acid based in situ detection of microRNAs in mouse tissue sections. Nat Protoc, 2, 1508-14. https://doi.org/10.1038/nprot.2007.153
  28. Orang AV, Safaralizadeh R, Hosseinpour Feizi MA (2014). Insights into the diverse roles of miR-205 in human cancers. Asian Pac J Cancer Prev, 15, 577-83. https://doi.org/10.7314/APJCP.2014.15.2.577
  29. Ouyang L, Shi Z, Zhao S, et al (2012). Programmed cell death pathways in cancer: a review of apoptosis, autophagy and programmed necrosis. Cell Prolif, 45, 487-98. https://doi.org/10.1111/j.1365-2184.2012.00845.x
  30. Politz JC, Zhang F, Pederson T (2006). MicroRNA-206 colocalizes with ribosome-rich regions in both the nucleolus and cytoplasm of rat myogenic cells. Proc Natl Acad Sci USA, 103, 18957-62. https://doi.org/10.1073/pnas.0609466103
  31. Sassen S, Miska EA, Caldas C (2008). MicroRNA: implications for cancer. Virchows Arch, 452, 1-10.
  32. Schwarz S, Stiegler C, Muller M, et al (2011). Salivary gland mucoepidermoid carcinoma is a clinically, morphologically and genetically heterogeneous entity: a clinicopathological study of 40 cases with emphasis on grading, histological variants and presence of the t(11;19) translocation. Histopathology, 58, 557-70. https://doi.org/10.1111/j.1365-2559.2011.03777.x
  33. Spizzo R, Nicoloso MS, Croce CM, et al (2009). SnapShot: MicroRNAs in Cancer. Cell, 137, 586 https://doi.org/10.1016/j.cell.2009.04.040
  34. Su N, Qiu H, Chen Y, et al (2013). miR-205 promotes tumor proliferation and invasion through targeting ESRRG in endometrial carcinoma. Oncol Rep, 29, 2297-302.
  35. Tran N, McLean T, Zhang X, et al (2007). MicroRNA expression profiles in head and neck cancer cell lines. Biochem Biophys Res Commun, 358, 12-7. https://doi.org/10.1016/j.bbrc.2007.03.201
  36. Tran N, O'Brien CJ, Clark J, et al (2010). Potential role of microRNAs in head and neck tumorigenesis. Head Neck, 32, 1099-111. https://doi.org/10.1002/hed.21356
  37. Tu HF, Liu CJ, Chang CL, et al (2012). The association between genetic polymorphism and the processing efficiency of miR-149 affects the prognosis of patients with head and neck squamous cell carcinoma. PLoS One, 7, 51606. https://doi.org/10.1371/journal.pone.0051606
  38. Ventura A, Jacks T (2009). MicroRNAs and cancer: short RNAs go a long way. Cell, 136, 586-91. https://doi.org/10.1016/j.cell.2009.02.005
  39. Wang N, Li Q, Feng NH, et al (2013). miR-205 is frequently downregulated in prostate cancer and acts as a tumor suppressor by inhibiting tumor growth. Asian J Androl, 15, 735-41. https://doi.org/10.1038/aja.2013.80
  40. Wolfish EB, Nelson BL, Thompson LD (2012). Sinonasal tract mucoepidermoid carcinoma: a clinicopathologic and immunophenotypic study of 19 cases combined with a comprehensive review of the literature. Head Neck Pathol, 6, 191-207. https://doi.org/10.1007/s12105-011-0320-9
  41. Xie H, Zhao Y, Caramuta S, et al (2012). miR-205 expression promotes cell proliferation and migration of human cervical cancer cells. PLoS One, 7, 46990. https://doi.org/10.1371/journal.pone.0046990
  42. Zhang L, Sullivan PS, Goodman JC, et al (2011). MicroRNA-1258 suppresses breast cancer brain metastasis by targeting heparanase. Cancer Res, 71, 645-54. https://doi.org/10.1158/0008-5472.CAN-10-1910

Cited by

  1. Landscape of expression profiles in esophageal carcinoma by The Cancer Genome Atlas data vol.29, pp.8, 2015, https://doi.org/10.1111/dote.12416
  2. miR-205 promotes proliferation and invasion of laryngeal squamous cell carcinoma by suppressing CDK2AP1 expression vol.48, pp.1, 2015, https://doi.org/10.1186/s40659-015-0052-5