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Isolation and Characterization of α-Glucosidase Inhibitor Produced by Bacillus sp. SKU31-1 Strain

Bacillus sp. SKU31-1가 생산하는 α-Glucosidase 저해제 분리 및 특성 조사

  • Kim, Shin-Duk (Department of Chemical and Biological Engineering, Seokyeong University)
  • 김신덕 (서경대학교 화학생명공학과)
  • Received : 2014.12.01
  • Accepted : 2014.12.18
  • Published : 2014.12.31

Abstract

In the course of screening for ${\alpha}$-glucosidase inhibitor produced by microorganism, the active compound was isolated from the culture filtrate of Bacillus sp. SKU31-1 using a series of chromatography procedures. The structure of the active compound was elucidated as 5-amino-1-hydroxymethyl-1, 2, 3, 4-cyclohexanetetrol on the basis of spectroscopic evidence obtained and comparison with data from the literature. The active compound showed potent inhibitory activity against ${\alpha}$-glucosidase with an $IC_{50}$ value of $1.9{\mu}M$ for maltose and 4.9 mM for sucrose. A Lineweaver-Burk plot indicated that its inhibition of ${\alpha}$-glucosidase was competitive, with a $K_i$ value of 0.15 mM.

Keywords

Bacillus sp.;competitive inhibitor;${\alpha}$-glucosidase inhibitor;valiolamine

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  1. α-Glucosidase inhibitory activity and protease characteristics produced by Bacillus amyloliquefaciens vol.22, pp.5, 2015, https://doi.org/10.11002/kjfp.2015.22.5.727