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Characterization of Biogenic Amine-reducing Pediococcus pentosaceus Isolated from Traditionally Fermented Soybean Products

전통 장류에서 분리한 Biogenic Amines 저감 유산균 Pediococcus pentosaceus의 분리 및 특성

  • Oh, HyeonHwa (Department of Biological Sciences, Chonbuk National University) ;
  • Ryu, MyeongSeon (Department of Biological Sciences, Chonbuk National University) ;
  • Heo, Jun (Department of Biological Sciences, Chonbuk National University) ;
  • Jeon, SaeBom (Department of Biological Sciences, Chonbuk National University) ;
  • Kim, Young Sang (Ministry of Food and Drug Safety (MFDS)) ;
  • Jeong, DoYoun (Microbial Institute for Fermentation Industry (MIFI)) ;
  • Uhm, Tai-Boong (Department of Biological Sciences, Chonbuk National University)
  • 오현화 (전북대학교 자연과학대학 생물학과) ;
  • 류명선 (전북대학교 자연과학대학 생물학과) ;
  • 허준 (전북대학교 자연과학대학 생물학과) ;
  • 전새봄 (전북대학교 자연과학대학 생물학과) ;
  • 김용상 (식품의약품안전처) ;
  • 정도연 (발효미생물산업진흥원) ;
  • 엄태붕 (전북대학교 자연과학대학 생물학과)
  • Received : 2014.08.29
  • Accepted : 2014.09.24
  • Published : 2014.12.31

Abstract

Two bacterial strains, named as LE17 and LE22, were isolated from traditionally fermented soybean products in order to select lactic acid bacteria for the reduction of biogenic amines and harmful bacteria. Both strains were identified as Pediococcus pentosaceus by 16S rRNA sequence analysis and additional biochemical tests. The strain LE17 reduced the amines by 13.7% for histamine and by 25.9% for tyramine, when it grew in minimal synthetic media containing 0.1% (w/v) histamine and 0.1% tyramine at $30^{\circ}C$ for 48 h, while the strain LE22 reduced the amines by 23.7% for histamine and by 15.7% for tyramine. Both strains also had broad inhibition spectra against pathogens. Considering their properties, they could be used as starters for industrial soybean fermentation.

Keywords

Pediococcus;anti-bacterial agents;biogenic amines;lactic acid bacteria;soybean products

Acknowledgement

Supported by : 농림수산식품부

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