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Interactions between Indole-3-acetic Acid Producing Acinetobacter sp. SW5 and Growth of Tomato Plant

Indole-3-acetic acid를 생성하는 Acinetobacter sp. SW5와 토마토 식물 간의 상호작용

  • Kwon, Hyeok-Do (Department of Biological Sciences, Kangwon National University) ;
  • Song, Hong-Gyu (Department of Biological Sciences, Kangwon National University)
  • Received : 2014.08.12
  • Accepted : 2014.10.26
  • Published : 2014.12.31

Abstract

Many rhizobacteria can promote plant growth through various direct or indirect mechanisms, and their production of phytohormones such as indole-3-acetic acid (IAA) may have pronounced effects on growth and development of plants. Rhizobacterial strain isolated from rhizosphere of foxtail (Setaria viridis), Acinetobacter sp. SW5 produced 118.1 mg/L of IAA and 4.5 mg/L of gibberellin ($GA_3$) in brain heart broth medium at 2 and 1 day of incubation, respectively. In a pot test the lengths of stem and root and fresh weight of the germinated tomato seedlings treated with Acinetobacter sp. SW5 significantly increased by 26.3, 33.3, and 105.3%, respectively compared to those of the uninoculated control in 12 weeks of cultivation. When the root exudate secreted from tomato seedlings was analyzed by HPLC, 3.75 ng mg tomato $root^{-1}$ of tryptophan which is an IAA precursor was detected. Acinetobacter sp. SW5 could produce $4.06{\mu}M$ of IAA from root exudate from 8 tomato seedlings. Together with the capability of growth of Acinetobacter sp. SW5 in the tomato root exudates, this IAA secreted by bacteria might contribute to enhance the growth of tomato plants.

Keywords

Acinetobacter sp. SW5;indole acetic acid;plant growth promotion;tomato root exudates;tryptophan

Acknowledgement

Supported by : Kangwon National University

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