- Volume 50 Issue 4
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Antiviral Properties of Probiotic Mixtures against Rotavirus in the Rat
랫드에서 로타바이러스에 대한 유산균혼합물의 항 바이러스활성
- Park, Jae Eun (College of Pharmacy, Sahmyook University) ;
- Lee, Do Kyung (College of Pharmacy, Sahmyook University) ;
- Kim, Min Ji (College of Pharmacy, Sahmyook University) ;
- Kim, Kyung Tae (Department of Chemistry, Sahmyook University) ;
- Choi, Kyung Soon (Department of Food and Nutrition, Sahmyook University) ;
- Seo, Jae Goo (R&D Center, Cellbiotech, Co. Ltd.) ;
- Ha, Nam Joo (College of Pharmacy, Sahmyook University)
- 박재은 (삼육대학교 약학과) ;
- 이도경 (삼육대학교 약학과) ;
- 김민지 (삼육대학교 약학과) ;
- 김경태 (삼육대학교 화학과) ;
- 최경순 (삼육대학교 식품영양학과) ;
- 서재구 ((주)쎌바이오텍) ;
- 하남주 (삼육대학교 약학과)
- Received : 2014.09.05
- Accepted : 2014.12.11
- Published : 2014.12.31
Rotavirus is a major cause of acute gastroenteritis in young children in developed and developing countries. The use of probiotics for the treatment of gastrointestinal diseases is both safe and easily accessible. In this study, we evaluated the anti-rotaviral activities of probiotic mixtures in a Sprague-Dawley rat. 24 litters with their dams were randomly assigned to four groups; placebo, phosphate buffered saline (PBS), and two probiotic mixture (PRO-1 and PRO-2) groups. All rats were inoculated with rotavirus at dose of 8 log plaque forming units per rat at 5 days old. Animals in the PRO-1 and PRO-2 groups were orally administered probiotic mixtures 1 or 2, respectively, at a dose of 8 log colony forming units daily during 4 days. For control purposes, placebo and PBS groups were orally administered the same amount of placebo (containing maltose and polydextrose) or PBS once daily for 4 days, respectively. Antiviral analysis was performed by real-time quantitative PCR (RT-qPCR) and observing intestinal villi. As a result, weights of small intestines were greater in the PRO-1, PRO-2 groups than in control groups. Villi were short and villous epithelial necrosis was exhibited in control groups, but these morphological changes were not observed in PRO-1, PRO-2 treated rats. RT-qPCR analysis showed that VP7 gene level of rotavirus in fecal samples and small intestinal epithelial cells were lower in the PRO-1 and PRO-2 groups. These findings suggest that probiotic mixtures may be useful probiotics for the treatment of or as alternative therapies for rotaviral gastroenteritis.
anti-rotaviral activities;probiotics;real-time quantitative PCR (RT-qPCR)
Supported by : Sahmyook University
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