DOI QR코드

DOI QR Code

Analysis of Epidemiological Characteristics, PFGE Typing and Antibiotic Resistance of Pathogenic Escherichia coli Strains Isolated from Gyeonggi-do

경기도에서 분리한 병원성대장균의 역학적 특성 및 PFGE, 항생제 내성 연구

  • Kim, Kyung-A (Team of Microbiology, Gyeonggi Institute of Health and Environment) ;
  • Yong, Kum-Chan (Team of Microbiology, Gyeonggi Institute of Health and Environment) ;
  • Jeong, Jin-A (Team of Microbiology, Gyeonggi Institute of Health and Environment) ;
  • Huh, Jeong-Weon (Team of Microbiology, Gyeonggi Institute of Health and Environment) ;
  • Hur, Eun-Seon (Team of Microbiology, Gyeonggi Institute of Health and Environment) ;
  • Park, Sung-Hee (Team of Microbiology, Gyeonggi Institute of Health and Environment) ;
  • Choi, Yun-Sook (Team of Microbiology, Gyeonggi Institute of Health and Environment) ;
  • Yoon, Mi-Hye (Team of Microbiology, Gyeonggi Institute of Health and Environment) ;
  • Lee, Jong-Bok (Team of Microbiology, Gyeonggi Institute of Health and Environment)
  • 김경아 (경기도보건환경연구원 미생물팀) ;
  • 용금찬 (경기도보건환경연구원 미생물팀) ;
  • 정진아 (경기도보건환경연구원 미생물팀) ;
  • 허정원 (경기도보건환경연구원 미생물팀) ;
  • 허은선 (경기도보건환경연구원 미생물팀) ;
  • 박성희 (경기도보건환경연구원 미생물팀) ;
  • 최연숙 (경기도보건환경연구원 미생물팀) ;
  • 윤미혜 (경기도보건환경연구원 미생물팀) ;
  • 이정복 (경기도보건환경연구원 미생물팀)
  • Received : 2014.08.29
  • Accepted : 2014.11.26
  • Published : 2014.12.31

Abstract

This study was conducted to survey the epidemiological characteristics and the isolated strains for pathogenic E. coli which was the major causative organisms for food poisoning occurred at school food services in the Gyeonggi-do area during the past three years. We investigated 19 accidents of food-borne disease outbreaks by pathogenic E. coli at school food services from 2010 to 2012. Food-borne disease outbreaks by pathogenic E. coli were usually occurred at direct management type (18 accidents, 95%) and high schools. For the seasonal factors, 13 accidents (65%) were occurred in June to September, especially the end of August and September after the summer holidays. The first patients were occurred on Wednesday (7 accidents, 37%) and Thursday (7 accidents, 37%), and they were mainly reported on Thursday (7 accidents, 37%) and Friday (5 accidents, 26%). The exposure of risk was estimated in Monday (4 accidents, 21%), Tuesday (7 accidents, 37%) and Wednesday (4 accidents, 21%), and kimchi (5 accidents, 50%) was estimated as the food of the high risk responsible for the outbreaks. 98 isolates of pathogenic E. coli consisted of PEC (50%), ETEC (34%), EAEC (15%), and EHEC (1%). The antibiotic resistance of pathogenic E. coli showed in the descending order of ampicilline (40%), nalidixic acid (37%), trimethoprim/sulfamethoxazole (24%), and tetracycline (19%). The antibiotics of second and third generation cephalosporins, cabarpenem, aminoglycosides, and second generation quinolones had antimicrobial susceptibilities and cefalotin, ampicillin/sulbactam and chloramphenicol showed medium resistance at 29%, 25%, and 6% respectively, and 70% of isolates were resistant to more than one antibiotic. By the PFGE analysis, they were classified into nine major groups and 31 profiles with 57% pattern similarity. It was very difficult to find the correlation of antimicrobial susceptibilities and genotype in the small scale-food poisoning, but the similarity of antimicrobial resistance and PFGE patterns in the large scale-food poisoning enabled the outbreaks to estimate the same pathotype of E. coli derived from identical origins.

Keywords

antimicrobial susceptibility;pathogenic E. coli;PFGE;school food service

References

  1. Bentley, R. and Meganathan, R. 1982. Biosynthesis of vitamin K (menaquinone) in Bacteria. Bacteriol. Rev. 46, 241-280.
  2. Dixon B. 2000. Antimicrobials as growth promoters : Risks and alternatives. ASM News 66, 264-265.
  3. Dupont, H.L., Formal, S.B., Hornick, R.B., Snyder, M.J., Libonati, J.P., and Sheahan, D.G. 1971. Pathogenesis of Escherichia coli diarrhea. N. Engl. J. Med. 285, 1-9. https://doi.org/10.1056/NEJM197107012850101
  4. e-Country Indicators. 2012. http://www.index.go.kr/search/search.jsp.
  5. Finlay, B.B., Rosenshine, I., Donnenberg, M.S., and Kaper, J.B. 1992. Cytoskeletal composition of attaching and effacing lesions associated with enteropathogenic Escherichia coli adherence to HeLa cells. Infect. Immun. 60, 2541-2543.
  6. Hudault, S., Guignot, J., and Servin, A.L. 2001. Escherichia coli strains colonizing the gastrointestinal tract protect germfree mice against Salmonella typhimurium infection. Gut 49, 47-55. https://doi.org/10.1136/gut.49.1.47
  7. Huh, M.J., Oh, S.S., and Jang, J.S. 2013. Antimicrobial resistance and implicated genes of E. coli isolated from commercial and cooked foods in Seoul. Korean J. Food Nutr. 26, 132-136. https://doi.org/10.9799/ksfan.2013.26.1.132
  8. Jin, Y.H., Seung, H.J., Oh, Y.H., Jung, J.H., Jeon, S.J., Lee, J.K., Kim, C.K., Choi, S.M., and Chae, Y.Z. 2013. Epidemiological relationship of enterotoxigenic Escherichia coli and enteroaggregative E. coli isolated from patients with diarrhea in Seoul. J. Bacteriol. Virol. 43, 37-44. https://doi.org/10.4167/jbv.2013.43.1.37
  9. Jo, S.H., Kim, C.l., and Ha, S.D. 2009. Outbreak pattern forecasting of food-borne disease in group food services in Korea. J. Fd. Hyg. Safety 24, 19-26.
  10. Jung, S.C. 2005. Screening of antimicrobial resistance. The 3rd national antimicrobial resistance safety management and vision. Korea Food and Drug Administration, pp. 113-126.
  11. Kaper, J.B., Nataro, J.P., and Mobley, H.L.T. 2004. Pathogenic Escherichia coli. Nat. Rev. Microbiol. 2, 123-140. https://doi.org/10.1038/nrmicro818
  12. Kim, J.H., Oh, B.Y., Gong, Y.W., Kim, H.Y., Lee, M.Y., Koh, Y.J., Hwang, K.W., Jegal, S., and Lee, J.M. 2010. The trend of antimicrobial resistance of Escherichia coli isolated from healthy volunteers of community and hospital patients in Incheon. Korean J. Microbiol. 42, 252-256.
  13. Kim, Y.J., Chun, H.J., Lee, J.W., Hong, K.W., Kim, S.I., Wie, S.H., Kim, Y.R., and Kang, M.W. 2012. The changing patterns of antibiotics usage in Korea during 1981-2008. Infect. Chemother. 44, 411-418. https://doi.org/10.3947/ic.2012.44.6.411
  14. Korea Food Safety Research Institute. 2013. Media Workshop 2013 12th school meals and Safety. http://www.kfsri.or.kr/.
  15. Korea Ministry of Agriculture, Food and Rural Affairs. 2013. http://www.mafra.go.kr https://www.mafra.go.kr/list.jsp?&newsid= 155444846§ion_id=b_sec_1&pageNo=88&year=2014&list cnt=10&board_kind=C&board_skin_id=C3&depth=1&division =B&group_id=3&menu_id=1125&reference=2&parent_code=3 &popup_yn=N&tab_yn=N.
  16. Korea Ministry of Food and Drug Safety. 2012a. http://www.mfds.go.kr/ e-stat/index.do.
  17. Korea Ministry of Health and Welfare. 2009. http://www.mw.go.kr/ front_new/al/sal0301vw.jsp?PAR_MENU_ID=04&MENU_ID= 0403&BOARD_ID=140&BOARD_FLAG=00&CONT_SEQ=2 17331&page=1
  18. Knutton, S. 1994. Attaching and effacing E. coli. In Gyles, C.L. (ed.), Escherichia coli in domestic animals and humans, pp. 567-591. CAB International, Wallingford.
  19. Lee, K.M. and Ryu, K. 2007. Field assessment of sanitation management for school foodservice suppliers in the Seoul area. Kor. J. Food Cookery Sci. 23, 650-663.
  20. McDaniel, T.K., Jarvis, K.G., Donnenberg, M.S., and Kaper, J.B. 1995. A genetic locus of enterocyte effacement conserved among diverse enterobacterial pathogens. Proc. Natl. Acad. Sci. USA 92, 1664-1668. https://doi.org/10.1073/pnas.92.5.1664
  21. Nataro, J.P. and Kaper, J.B. 1998. A comprehensive review of the pathogenesis, epidemiology, diagnosis and clinical aspects of diarrhoeagenic E. coli. Clin. Microbiol. Rev. 11, 142-201.
  22. Ochiai, K., Yamanaka, T., Kimura, K., and Sawada, O. 1959. Inheritance of drug resistance (and its transfer) between Shigella strains and between Shigella and E. coli strains. Hihon Iji Shimpor 1861, 34.
  23. People's solidarity for participatory democracy. Antibiotics Usage monitoring at livestock industry and aquaculture II. 2006. http://www.peoplepower21.org/StableLife/629493.
  24. Pupo, G.M., Lan, R., and Reeves, P.R. 2000. Multiple independent origins of Shigella clones of Escherichia coli and convergent evolution of many of their characteristics. Proc. Natl. Acad. Sci. USA 97, 10567-10572. https://doi.org/10.1073/pnas.180094797
  25. Reid, G., Howard, J., and Gan, B.S. 2001. Can bacterial interference prevent infection? Trends Microbiol. 9, 424-428. https://doi.org/10.1016/S0966-842X(01)02132-1
  26. Sansonetti, P. 2002. Host-pathogen interactions: the seduction of molecular cross talk. Gut 50, 2-8.
  27. Scaletsky, I.C.A., Fabbricotti, S.H., Carvalho, R.L.B., Nunes, C.R., Maranha, H.S., Morais, M.B., and Fagundes-Neto, U. 2002. Diffusely adherent Escherichia coli as a cause of acute diarrhea in young children in northeast Brazil: a case-control study. J. Clin. Microbiol. 40, 645-648. https://doi.org/10.1128/JCM.40.2.645-648.2002
  28. Sung, M.S., Kim, J.H., and Kim, K.S. 2010. Molecular epidemiologic analysis of pathogenic Escherichia coli isolated from poultry in Korea. Kor. J. Vet. Res. 50, 239-246.
  29. Wei, J., Goldberg, M.B., Burland V., Venkatesan, M.M., Deng, W., Fournier, G., Mayhew, G.F., Plunkett III, G., Rose, D.J., Darling, A., and et al. 2003. Complete genome sequence and comparative genomics of Shigella flexneri serotype 2a strain 2457T. Infect. Immun. 71, 2775-2786. https://doi.org/10.1128/IAI.71.5.2775-2786.2003
  30. Wolfgang, W. 1998. Medical consequences of antibiotic use in agriculture. Science 279, 996-997. https://doi.org/10.1126/science.279.5353.996
  31. Yoo, Y.A., Kim, M.S., Kim, K.S., Park, S.H., and Jung, S.K. 2010. Antimicrobial resistance and implicated genes of E. coli isolated from commercial and cooked foods in Seoul. J. Fd. Hyg. Safety 25, 220-225.
  32. Korea Ministry of Food and Drug Safety. 2012b. http://www.mfds.go.kr/ index.do?mid= 664&pageNo=92&seq=17557&cmd=v.

Cited by

  1. Strains in Kimchi and Determination of Their Kinetic Behavior in Cabbage Kimchi During Fermentation vol.15, pp.7, 2018, https://doi.org/10.1089/fpd.2017.2391
  2. Can Survive in Kimchi during Fermentation vol.81, pp.6, 2018, https://doi.org/10.4315/0362-028X.JFP-17-459
  3. in Foodborne Outbreak vol.48, pp.4, 2018, https://doi.org/10.4167/jbv.2018.48.4.113