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Proteomic Response of Alfalfa Subjected to Aluminum (Al) Stress at Low pH Soil

  • Rahman, Md. Atikur (Division of Applied Life Sciences (BK21Plus), IALS, PMBBRC, Gyeongsang National University) ;
  • Kim, Yong-Goo (Division of Applied Life Sciences (BK21Plus), IALS, PMBBRC, Gyeongsang National University) ;
  • Lee, Byung-Hyun (Division of Applied Life Sciences (BK21Plus), IALS, PMBBRC, Gyeongsang National University)
  • Received : 2014.12.16
  • Accepted : 2014.12.31
  • Published : 2014.12.31

Abstract

In order to reveal the aluminum (Al) stress tolerance mechanisms in alfalfa plant at low pH soil, a proteomic approach has been conducted. Alfalfa plants were exposed to Al stress for 5 days. The plant growth and total chlorophyll content are greatly affected by Al stress. The malondialdehyde (MDA) and $H_2O_2$ contents were increased in a low amount but free proline and soluble sugar contents, and the DPPH-radical scavenging activity were highly increased. These results indicate that antioxidant activity (DPPH activity) and osmoprotectants (proline and sugar) may involve in ROS ($H_2O_2$) homeostasis under Al stress. In proteomic analysis, over 500 protein spots were detected by 2-dimentional gel electrophoresis analysis. Total 17 Al stress-induced proteins were identified, of which 8 protein spots were up-regulated and 9 were down-regulated. The differential expression patterns of protein spots were selected and analyzed by the peptide mass fingerprinting (PMF) using MALDI-TOF MS analysis. Three protein spots corresponding to Rubisco were significantly down-regulated whereas peroxiredoxin and glutamine synthetase were up-regulated in response to Al stress. The different regulation patterns of identified proteins were involved in energy metabolism and antioxidant / ROS detoxification during Al stress in alfalfa. Taken together, these results provide new insight to understand the molecular mechanisms of alfalfa plant in terms of Al stress tolerance.

Keywords

Alfalfa;Aluminum stress;Proteome

Acknowledgement

Supported by : National Research Foundation (NRF), Rural Development Administration

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