Dietary Supplementation of Benzoic Acid and Essential Oil Compounds Affects Buffering Capacity of the Feeds, Performance of Turkey Poults and Their Antioxidant Status, pH in the Digestive Tract, Intestinal Microbiota and Morphology

  • Giannenas, I. (Laboratory of Nutrition, School of Veterinary Medicine, Aristotle University of Thessaloniki PC 54124) ;
  • Papaneophytou, C.P. (Laboratory of Biochemistry, Veterinary Faculty, University of Thessaly) ;
  • Tsalie, E. (Laboratory of Pathology, Veterinary Faculty, University of Thessaly) ;
  • Pappas, I. (Laboratoty of Pharmacology, Veterinary Faculty, University of Thessaly) ;
  • Triantafillou, E. (Military Veterinary Training and Nursing Center) ;
  • Tontis, D. (Laboratory of Pathology, Veterinary Faculty, University of Thessaly) ;
  • Kontopidis, G.A. (Laboratory of Biochemistry, Veterinary Faculty, University of Thessaly)
  • Received : 2013.07.01
  • Accepted : 2013.09.21
  • Published : 2014.02.01


Three trials were conducted to evaluate the effect of supplementation of a basal diet with benzoic acid or thymol or a mixture of essential oil blends (MEO) or a combination of benzoic acid with MEO (BMEO) on growth performance of turkey poults. Control groups were fed a basal diet. In trial 1, benzoic acid was supplied at levels of 300 and 1,000 mg/kg. In trial 2, thymol or the MEO were supplied at levels of 30 mg/kg. In trial 3, the combination of benzoic acid with MEO was evaluated. Benzoic acid, MEO and BMEO improved performance, increased lactic acid bacteria populations and decreased coliform bacteria in the caeca. Thymol, MEO and BMEO improved antioxidant status of turkeys. Benzoic acid and BMEO reduced the buffering capacity compared to control feed and the pH values of the caecal content. Benzoic acid and EOs may be suggested as an effective alternative to AGP in turkeys.


Feed Additives;Benzoic Acid;Essential Oils;Turkeys;Performance


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