- Volume 27 Issue 2
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Dietary Supplementation of Benzoic Acid and Essential Oil Compounds Affects Buffering Capacity of the Feeds, Performance of Turkey Poults and Their Antioxidant Status, pH in the Digestive Tract, Intestinal Microbiota and Morphology
- Giannenas, I. (Laboratory of Nutrition, School of Veterinary Medicine, Aristotle University of Thessaloniki PC 54124) ;
- Papaneophytou, C.P. (Laboratory of Biochemistry, Veterinary Faculty, University of Thessaly) ;
- Tsalie, E. (Laboratory of Pathology, Veterinary Faculty, University of Thessaly) ;
- Pappas, I. (Laboratoty of Pharmacology, Veterinary Faculty, University of Thessaly) ;
- Triantafillou, E. (Military Veterinary Training and Nursing Center) ;
- Tontis, D. (Laboratory of Pathology, Veterinary Faculty, University of Thessaly) ;
- Kontopidis, G.A. (Laboratory of Biochemistry, Veterinary Faculty, University of Thessaly)
- Received : 2013.07.01
- Accepted : 2013.09.21
- Published : 2014.02.01
Three trials were conducted to evaluate the effect of supplementation of a basal diet with benzoic acid or thymol or a mixture of essential oil blends (MEO) or a combination of benzoic acid with MEO (BMEO) on growth performance of turkey poults. Control groups were fed a basal diet. In trial 1, benzoic acid was supplied at levels of 300 and 1,000 mg/kg. In trial 2, thymol or the MEO were supplied at levels of 30 mg/kg. In trial 3, the combination of benzoic acid with MEO was evaluated. Benzoic acid, MEO and BMEO improved performance, increased lactic acid bacteria populations and decreased coliform bacteria in the caeca. Thymol, MEO and BMEO improved antioxidant status of turkeys. Benzoic acid and BMEO reduced the buffering capacity compared to control feed and the pH values of the caecal content. Benzoic acid and EOs may be suggested as an effective alternative to AGP in turkeys.
Feed Additives;Benzoic Acid;Essential Oils;Turkeys;Performance
- AOAC. 1995. Official methods of analysis of the association of official analytical chemists, 16th ed. AOAC Int., Arlington, USA.
- Bosund, I. 1962. The action of benzoic and salicylic acids on the metabolism of microorganisms. Adv. Food Res. 11: 331-353.
- Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254. https://doi.org/10.1016/0003-2697(76)90527-3
- Brenes, A. and E. Roura. 2010. Essential oils in poultry nutrition: Main effects and modes of action. Anim. Feed Sci. Technol. 158:1-14. https://doi.org/10.1016/j.anifeedsci.2010.03.007
- Buege, J. A. and S. D. Aust. 1978. Microsomal lipid peroxidation. Meth. Enzymol. 52:302-310. https://doi.org/10.1016/S0076-6879(78)52032-6
- Buhler, K., C. Wenk, J. Broz, and S. Gebert. 2006. Influence of benzoic acid and dietary protein level on performance, nitrogen metabolism and urinary pH in growing-finishing pigs. Arch. Anim. Nutr. 60:382-389. https://doi.org/10.1080/17450390600884369
- Burt, S. 2004. Essential oils: their antibacterial properties and potential applications in foods - a review. Int. J. Food Microbiol. 94:223-253. https://doi.org/10.1016/j.ijfoodmicro.2004.03.022
- Cave, N. A. 1984. Effect of dietary propionic and lactic acids on feed intake by chicks. Poult. Sci. 63:131-134. https://doi.org/10.3382/ps.0630131
- Cook, R. H. and F. H. Bird. 1973. Duodenal villus area and epithelial cellular migration in conventional and germ-free chicks. Poult. Sci. 52:2276-2280. https://doi.org/10.3382/ps.0522276
- Falkowski, J. and F. X. Aherne. 1984. Fumaric acid and citric acid as feed additives in starter pig nutrition. J. Anim. Sci.58:935-938.
- Florou-Paneri, P., E. Christaki, N. A. Botsoglou, A. Kalousis, and A. B. Spais. 2001. Performance of broilers and the hydrogen ion concentration in their digestive tract following feeding of diets with different buffering capacities. Arch. Geflugelkd. 65: 1-5.
- Franz, C., K. H. C Baser, and W. Windisch. 2010. Essential oils and aromatic plants in animal feeding - a European perspective. A review. Flavour Fragr. J. 25:327-340. https://doi.org/10.1002/ffj.1967
- Friedman, M., P. R. Henika, and R. E. Mandrell. 2003. Antibacterial activities of phenolic benzaldehydes and benzoic acids against Campylobacter jejuni, Escherichia coli, Listeria monocytogenes and Salmonella enterica. J. Food Prot. 66: 1811-1821.
- Giannenas, I., P. Florou-Paneri, N. Botsoglou, E. Christaki, and A. B. Spais. 2005. Effect of supplementing feed with oregano and/or a-tocopheryl acetate on growth of broiler chickens and oxidative stability of meat. J. Anim. Feed Sci. 14:521-535.
- Giannenas, I., P. Florou-Paneri, M. Papazahariadou, E. Christaki, N. A. Botsoglou, and A. B. Spais. 2003. Effect of dietary supplementation with oregano essential oil on performance of broilers after experimental infection with Eimeria tenella. Arch. Anim. Nutr. 57:99-106. https://doi.org/10.1080/0003942031000107299
- Giannenas, I., E. Tsalie, E. Chronis, S. Mavridis, D. Tontis, and I. Kyriazakis. 2011. Consumption of Agaricus bisporus mushroom affects the performance, intestinal microflora composition and morphology, and antioxidant status of turkey poults. Anim. Feed Sci. Technol. 165:218-229. https://doi.org/10.1016/j.anifeedsci.2011.03.002
- Giesting, D. W. and R. A. Easter. 1985. Response of starter pigs to supplementation of corn-soybean meal diets with organic acids. J. Anim. Sci. 60:1288-1294.
- Jensen, B. B. 2001. Possible ways of modifying type and amount of products from microbial fermentation in the gut. In: Gut Environment of Pigs (Ed. A. Piva, K. E. Bach Knudsen, and J. E. Lindberg). The Nottingham University Press, Nottingham, pp. 181-200.
- Jiang, Z. Y., S. Q. Jiang, Y. C. Lin, P. B. Xi, D. Q. Yu, and T. X. Wu. 2007. Effects of soybean isoflavone on growth performance, meat quality, and antioxidation in male broilers. Poult. Sci. 86: 1356-1362. https://doi.org/10.1093/ps/86.7.1356
- Jin, L.Z., Y. W. Ho, N. Abdullah, and S. Jalaludin. 1998. Growth performance, intestinal microbial populations, and serum cholesterol of broilers fed diets containing Lactobacillus cultures. Poult. Sci. 77:1259-1265. https://doi.org/10.1093/ps/77.9.1259
- Józefiak, D., S. Kaczmarek, M. Bochenek, and A. Rutkowski. 2006. A note on effects of benzoic acid supplementation on the performance and microbiota populations of broiler chickens. J. Anim. Feed Sci. 16:252-256.
- Józefiak, D., S. Kaczmarek, and A. Rutkowski. 2010. The effects of benzoic acid supplementation on the performance of broiler chickens. J. Anim. Physiol. Anim. Nutr. 94:29-34. https://doi.org/10.1111/j.1439-0396.2008.00875.x
- Habig, W. H., M. J. Pabst, and W. B. Jakoby. 1974. Glutathione S-transferases: the first enzymatic step in mercapturic acid formation. J. Biol. Chem. 249:7130-7139.
- Kluge, H., J. Broz, and K. Eder. 2006. Effect of benzoic acid on growth performance, nutrient digestibility, nitrogen balance, gastrointestinal microflora and parameters of microbial metabolism in piglets. J. Anim Physiol. Anim. Nutr. 90:316-324. https://doi.org/10.1111/j.1439-0396.2005.00604.x
- Knarreborg, A., N. Miquel, T. Granli, and B. B. Jensen. 2002. Establishment and application of an in vitro methodology to study the effects of organic acids on coliform and lactic acid bacteria in the proximal part of the gastrointestinal tract of piglets. Anim. Feed Sci. Technol. 99:131-140. https://doi.org/10.1016/S0377-8401(02)00069-X
- Krebs, H. A., D. Wiggins, M. Stubbs, A. Sols, and F. Bedoya. 1983. Studies on the mechanism of the antifungal action of benzoate. Biochem. J. 214:657-663.
- Langeveld, W. T., E. J. A. Veldhuizen, and A. A. Burt. 2013. Synergy between essential oil components and antibiotics: a review. Crit. Rev. Microbiol. 1:1-19.
- Miles, R. D., G. B. Butcher, P. R. Henry, and R. C. Littell. 2006. Effect of antibiotic growth promoters on broiler performance, intestinal growth parameters, and quantitative morphology. Poult. Sci. 85:476-485. https://doi.org/10.1093/ps/85.3.476
- Mroz, Z., A. W. Jongbloed, K. H. Partanen, K. Vreman, P. A. Kemme, and J. Kogut. 2000. The effects of calcium benzoate in diets with or without organic acids on dietary buffering capacity, apparent digestibility, retention of nutrients, and manure characteristics in swine. J. Anim. Sci. 78:2622-2632.
- Nesheim, M. C. and J. D. Garlich. 1963. Studies on ornithine synthesis in relation to benzoic acid excretion in the domestic fowl. J. Nutr. 79:311-317.
- National Research Council. 1996. Guide for the care and use of laboratory animals institute of laboratory animal resources, Commission on Life Sciences, National Academy Press, Washington, DC.
- Paglia, D. E. and W. N. Valentine. 1967. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J. Lab. Cm. Med. 70:158-69.
- Papageorgiou, G., N. Botsoglou, A. Govaris, I. Giannenas, S. Iliadis, and E. Botsoglou. 2003. Effect of dietary oregano oil and alpha-tocopheryl acetate supplementation on iron-induced lipid oxidation of turkey breast, thigh, liver and heart tissues. J. Anim. Physiol. Anim. Nutr. 87:324-335. https://doi.org/10.1046/j.1439-0396.2003.00441.x
- Polonen, I. J., K. H. Partanen, T. K. Jalava, and V. F. Toivonen. 2000. Effect of dietary glycine and benzoate level on benzoate metabolism in mink (Mustela vison), blue fox (Alopex lagopus), and raccoon dog (Nyctereutes procyonoides). J. Anim. Sci. 78:976-986.
- Rehman, H., J. Böhm, and J. Zentek. 2006. Effects of diets with inulin and sucrose on the microbial fermentation in the gastrointestinal tract of broilers. Proc. Soc. Nutr. Physiol. 15: 155-158.
- Rolfe, R. D. 2000. The role of probiotic cultures in the control of gastrointestinal health. J. Nutr. 130:396S-402S.
- Salah, N., N. Miller, G. Paganga, L. Tijburg, G. Bollwell, and C. Rice-Evans. 1995. Polyphenolic flavanols as scavengers of aqueous phase radicals and as chain-breaking antoxidants. Arch. Biochem. Biophys. 322:339-346. https://doi.org/10.1006/abbi.1995.1473
- Statistical Analysis Systems (SAS) Institute. 1989. SAS User's Guide. SAS Institute, Cary, NC.
- Sturkie, P. D. 1976. Protein metabolism. In: Avian Physiology, 3rd Ed, (Ed. P. D. Sturkie), Spinger-Verlag, New York, pp. 233-251.
- van der Wielen, P. W., L. J. Lipman, F. van Knapen, and S. Biesterveld. 2002. Competitive exclusion of Salmonella enterica serovar Enteritidis by Lactobacillus crispatus and Clostridium lactatifermentans in a sequencing fed-batch culture. Appl. Environ. Microbiol. 68:555-559. https://doi.org/10.1128/AEM.68.2.555-559.2002
- Waldroup, A., S. Kaniawati, and A. Mauromustakos. 1995. Performance characteristics and microbiological aspects of broilers fed diets supplemented with organic acids. J. Food Prot. 58:482-489.
- Watkins, B. A. and B. F Miller. 1983. Competitive gut exclusion of avian pathogens by Lactobacillus acidophilus in gnotobiotic chicks. Poult. Sci. 62:1772-1779. https://doi.org/10.3382/ps.0621772
- Wegener, H., F. Aarestrup, L. Jensen, A. M. Hammerum, and F. Bager. 1998. The association between the use of antimicrobial growth promoters and development of resistance in pathogenic bacteria towards growth promoting and therapeutic antimicrobials. Anim. Feed Sci. Technol. 7:7-14.
- Wildgrube, M. and M. Zausch. 1971. Silierung von Zuckerru ben. Tierzucht 25:90-93.
- Yason, C. V., B. A. Summers, and K. A. Schat. 1987. Pathogenesis of rotavirus infection in various age groups of chickens and turkeys: pathology. Am. J. Vet. Res. 48:927-938.
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- in Weaned Piglets vol.2018, pp.2314-6141, 2018, https://doi.org/10.1155/2018/1829632
- Effects of two sources of Mexican oregano oil on performance, blood profile, carcass variables, and meat of broilers vol.47, pp.0, 2018, https://doi.org/10.1590/rbz4720170198
- Intestinal Microbiome-Metabolome Responses to Essential Oils in Piglets vol.9, pp.1664-302X, 2018, https://doi.org/10.3389/fmicb.2018.01988