The Net Energy Values of Corn, Dried Distillers Grains with Solubles and Wheat Bran for Laying Hens Using Indirect Calorimetry Method

  • Ning, D. ;
  • Yuan, J.M. ;
  • Wang, Y.W. ;
  • Peng, Y.Z. ;
  • Guo, Y.M.
  • Received : 2013.05.03
  • Accepted : 2013.08.23
  • Published : 2014.02.01


The present study was conducted to estimate the NE values of corn, dried distillers grains with solubles (DDGS) and wheat bran (WB) for laying hens based on an indirect calorimetry method and nitrogen balance measurements. A total of 576 twenty-eight-wk-old Dwarf Pink-shell laying hens were randomly assigned to four groups fed a basal diet (BD) or a combination of BD with 50% corn or 20% DDGS or 20% WB, with four replicates each. After a 7-d adaptation period, each replicate with 36 hens were kept in one of the two respiration chambers to measure the heat production (HP) for 6 days during the feeding period and subsequent 3-d fasting. The equilibrium fasting HP (FHP) provided an estimate of NE requirements for maintenance (NEm). The NE values of test feedstuffs was estimated using the difference method. Results showed that the heat increment that contributed 35.34 to 37.85% of ME intake was not influenced by experimental diets (p>0.05) when expressed as Mcal/kg of DM feed intake. Lighting increased the HP in hens in an fed-state. The FHP decreased over time (p<0.05) with the lowest value determined on the third day of starvation. No significant difference between treatments was found on FHP of d 3 (p>0.05). The estimated AME, AMEn, and NE values were 3.46, 3.44 and 2.25 Mcal/kg DM for corn, 3.11, 2.79, and 1.80 Mcal/kg DM for DDGS, 2.14, 2.10, and 1.14 Mcal/kg DM for WB, respectively. The net availability of AME of corn tended to be numerically higher than DDGS and WB (p = 0.096). In conclusion, compared with corn, the energy values of DDGS and WB were overestimated when expressed on an AME basis.


Corn;Dried Distillers Grains with Solubles;Indirect Calorimetry;Laying Hens;Net Energy;Wheat Bran


  1. AOAC. 1990. Official methods of analysis. 15th ed. Association of Official Analytical Chemists, Arlington, Virginia.
  2. Adeola, O. and H. Zhai. 2012. Metabolizable energy value of dried corn distillers grains and corn distillers grains with solubles for 6-week-old broiler chickens. Poult. Sci. 91:712-718.
  3. Birkett, S. and K. de Lange. 2001a. A computational framework for a nutrient flow representation of energy utilization by growing monogastric animals. Br. J. Nutr. 86:661-674.
  4. Birkett, S. and K. de Lange. 2001b. Limitations of conventional models and a conceptual framework for a nutrient flow representation of energy utilization by animals. Br. J. Nutr. 86:647-659.
  5. Brouwer, E. 1957. On simple formulae for calculating the heat expenditure and the quantities of carbohydrate and fat oxidized in metabolism of men and animals from gaseous exchange (oxygen intake and carbonic acid output) and urine-N. Acta Physiol. Pharmacol. Neerl. 6:795-802.
  6. Carre, B., B. Prevotel, and B. Leclercq. 1984. Cell wall content as a predictor of metabolisable energy value of poultry feedingstuffs. Br. Poult. Sci. 25:561-572.
  7. Jorgensen, H., X. Q. Zhao, K. E. Knudsen, and B. O. Eggum. 1996. The influence of dietary fibre source and level on the development of the gastrointestinal tract, digestibility and energy metabolism in broiler chickens. Br. J. Nutr. 75:379-395.
  8. Hill, F. W. and D. L. Anderson. 1958. Comparison of metabolizable energy and productive energy determinations with growing chicks. J. Nutr. 64:587-603.
  9. Latshaw, J. D. and J. S. Moritz. 2009. The partitioning of metabolizable energy by broiler chickens. Poult. Sci. 88:98-105.
  10. Li, Y., T. Ito, M. Nishibori, and S. Yamamoto. 1992. Effects of environmental temperature on heat production associated with food intake and on abdominal temperature in laying hens. Br. Poult. Sci. 33:113-122.
  11. Li, Y. Z., T. Ito, and S. Yamamoto. 1991. Use of limited daily access to food in measuring the heat production associated with food intake in laying hens. Br. Poult. Sci. 32:829-839.
  12. MacLeod, M. G. 1990. Energy and nitrogen intake, expenditure and retention at 20 degrees in growing fowl given diets with a wide range of energy and protein contents. Br. J. Nutr. 64:625-637.
  13. MacLeod, M. G. 1991. Fat deposition and heat production as responses to surplus dietary energy in fowls given a wide range of metabolisable energy:protein ratios. Br. Poult. Sci. 32:1097-1108.
  14. MacLeod, M. G., H. Lundy, and T. R. Jewitt. 1985. Heat production by the mature male turkey (Meleagris gallopavo): preliminary measurements in an automated, indirect, open-circuit multi-calorimeter system. Br. Poult. Sci. 26:325-333.
  15. Mathlouthi, N., M. Larbier, M. A. Mohamed, and M. Lessire. 2002. Performance of laying hens fed wheat, wheat-barley or wheat-barley-wheat bran based diets supplemented with xylanase. Can. J. Anim. Sci. 82:193-199.
  16. Musharaf, N. A. and J. D. Latshaw. 1999. Heat increment as affected by protein and amino acid nutrition. World Poult. Sci. 55:233-240.
  17. Ning, D., Y. M. Guo, Y. W. Wang, and Y. Z. Peng. 2013. Earlier metabolizable energy intake level influences heat production during the following 3-day fast in laying hens. Asian-Aust. J. Anim. Sci. 26:558-563.
  18. Noblet, J., H. Fortune, X. S. Shi, and S. Dubois. 1994a. Prediction of net energy value of feeds for growing pigs. J. Anim. Sci. 72:344-354.
  19. Noblet, J., X. S. Shi, and S. Dubois. 1994b. Effect of body weight on net energy value of feeds for growing pigs. J. Anim. Sci. 72:648-657.
  20. National Research Council (NRC). 1994. Committee on animal nutrition. Subcommittee on Poultry Nutrition. Nutrient Requirements of Poultry. 9th rev. ed. National Academy of Sciences, Washington, DC.
  21. O'Neill, S. J. B. and N. Jackson. 1974. The heat production of hens and cockerels maintained for an extended period of time at a constant environmental temperature of $23{^{\circ}C}$. J. Agric. Sci. 82:549-552.
  22. Pishnamazi, A., J. Pourreza, M. A. Edriss, and A. H. Samie. 2005. Influence of broiler breeder and laying hen breed on the apparent metabolizable energy of selected feed ingredients. Int. J. Poult. Sci. 4:163-166.
  23. Sakomura, N. K., R. Basaglia, C. M. L. Sá-Fortes, and J. B. K. Fernandes. 2005. Model for metabolizable energy requirements of laying hens. R. Bras. Zootec. 34:575-583.
  24. Sarmiento-Franco, L., M. G. Macleod, and J. M. Mcnab. 2000. True metabolisable energy, heat increment and net energy values of two high fibre foodstuffs in cockerels. Br. Poult. Sci. 41:625-629.
  25. Shannon, D. W. and W. O. Brown. 1969. Losses of energy and nitrogen on drying poultry excreta. Poult. Sci. 48:41-43.
  26. Spratt, R. S., H. S. Bayley, B. W. McBride, and S. Leeson. 1990. Energy metabolism of broiler breeder hens. 1. The partition of dietary energy intake. Poult. Sci. 69:1339-1347.
  27. Sugahara, K. and T. Kubo. 1992. Involvement of food intake in the decreased energy retention associated with single deficiencies of lysine and sulphur-containing amino acids in growing chicks. Br. Poult. Sci. 33:805-814.
  28. van Soest, P. J. and R. H. Wine. 1967. Use of detergents in the analysis of fibrous feeds. I. Preparation of fibre residues of low nitrogen content.VI.Determination of cell-wall constituents. J. Assoc. Offic. Anal. Chem. 50:50-55.
  29. van Milgen, J., J. Noblet, S. Dubois, and J. F. Bernier. 1997. Dynamic aspects of oxygen consumption and carbon dioxide production in swine. Br. J. Nutr. 78:397-410.
  30. Wang, X., G. Jia, X. Li, and K. N. Wang. 2010a. Determination of the net energy and appropriate substitution ratio of soybean meal yellow-feathered broilers for using a substitution method. Chinese J. Anim. Nutr. 22:1434-1439.
  31. Wang X. L., G. M. Zhang, J. Q. Wang, and E. P. Zhang. 2010b. Measurement on net energy value of corn and soybean meal for laying hens. J. Northwest A & F University - Nat. Sci. Ed. 38:71-76.
  32. Zhang, Q. L., G. Jia, X. Q. Wu, and K. N. Wang. 2011. Determination of the net energy values of feedstuffs and its influence on performance and nitrogen availability of yellow-feather broilers aged from 1 to 21 days. Chinese J. Anim. Nutr. 23:1094-1100.

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