Effects of Dietary Levels of Glycine, Threonine and Protein on Threonine Efficiency and Threonine Dehydrogenase Activity in Hepatic Mitochondria of Chicks

  • Lee, C.W. (Institute of Marine BioTechnology, Pusan National University) ;
  • Cho, I.J. (College of Korean Medicine, Daegu Haany University) ;
  • Lee, Y.J. (College of Korean Medicine, Daegu Haany University) ;
  • Son, Y.S. (Division of Life Sciences, College of Life Sciences and Biotechnology, Korea University) ;
  • Kwak, I. (Department of Biological Sciences, Silla University) ;
  • Ahn, Y.T. (Institute of Marine BioTechnology, Pusan National University) ;
  • Kim, S.C. (College of Korean Medicine, Daegu Haany University) ;
  • An, W.G. (Institute of Marine BioTechnology, Pusan National University)
  • Received : 2012.10.20
  • Accepted : 2013.09.26
  • Published : 2014.01.01


This study was carried out to evaluate the relationship between threonine (Thr) efficiency and Thr dehydrogenase (TDG) activity as an indicator of Thr oxidation on chicks fed with levels of diets (CP [17.5% and 21.5%] and Thr [3.8 and 4.7 g/100 g CP]; glycine [Gly][0.64% and 0.98%] and true digestible Thr [dThr] [0.45% and 0.60%]). Calculation of the Thr efficiency was based on N-balance data and an exponential N-utilization model, and TDG activity was determined as accumulation of aminoacetone and Gly during incubation of hepatic mitochondria. This study found that in the liver of chicks who received a diet containing up to 0.79% Thr (4.7 g Thr/100 g of CP) in the 17.5% CP diet, no significant (p>0.05) effect on TDG activity was observed. However, significantly (p = 0.014) increased TDG activity was observed with a diet containing 21.5% CP (4.7 g Thr/100 g of CP) and the efficiency of Thr utilization showed a significant (p = 0.001) decrease, indicating the end of the Thr limiting range. No significant (p>0.05) effect on the total TDG activity and accumulation of Gly was observed with addition of Gly to a diet containing 0.45% dThr. In addition, addition of Gly to a diet containing 0.60% dThr also did not result in a change in accumulation of Gly. Due to an increase in accumulation of aminoacetone, an elevated effect on total TDG activity was also observed. No significant (p>0.05) reduction in the efficiency of Thr utilization was observed after addition of Gly at the level of 0.45% dThr. However, significantly (p<0.001) reduced efficiency of Thr utilization was observed after addition of Gly at the level of 0.60% dThr. Collectively, we found that TDG was stimulated not only by addition of Thr and protein to the diet, but also by addition of Gly, and efficiency of Thr utilization was favorably affected by addition of Gly at the level near to the optimal Thr concentration. In addition, no metabolic requirement of Gly through the TDG pathway was observed with almost the same accumulation of Gly and a slight increase in TDG activity by addition of Gly. Thus, our findings suggest that determination of TDG activity and parameter of efficiency of Thr utilization may be useful for evaluation of dietary Thr level.


Supported by : National Research Foundation of Korea (NRF)


  1. Asai, S., H. Nakamura, W. Okada, and M. Yamada. 1995. Synthesis of hippuric acid with inverse phase transfer catalyst in a heterogeneous liquid-liquid reaction system. Chem. Eng. Sci. 50:943-949.
  2. Ayasan, T., F. Okan, and H. Hizli. 2009. Threonine requirement of broilers from 22 to 42 days. Int. J. Poult. Sci. 8:862-865.
  3. Baker, D. H. and Y. Han. 1994. Ideal amino acid profile for chicks during the first three weeks posthatching. Poult. Sci. 73:1441-1447.
  4. Baylan, M., S. Canogullari, T. Ayasan, and A. Sahin. 2006. Dietary threonine supplementation for improving growth performance and edible carcass parts in Japanese quails, Coturnix coturnix japonica. Int. J. Poult. Sci. 5:635-638.
  5. Corzo, A., M. T. Kidd, D. J. Burnham, and B. J. Kerr. 2004. Dietary glycine needs of broiler chicks. Poult. Sci. 83:1382-1384.
  6. Corzo, A., M. T. Kidd, W. A. Dozier, and B. J. Kerr. 2009. Dietary glycine and threonine interactive effects in broilers. J. Appl. Poult. Res. 18:79-84.
  7. Davis, A. J. and R. E. Austic. 1994. Dietary threonine imbalance alters threonine dehydrogenase activity in isolated hepatic mitochondria of chicks and rats. J. Nutr. 124:1667 -1677.
  8. Davis, A. J. and R. E. Austic. 1997. Dietary protein and amino acid levels alter threonine dehydrogenase activity in hepatic mitochondria of Gallus domesticus. J. Nutr. 127:738-744.
  9. Dean, D. W., T. D. Bidner, and L. L. Southern. 2006. Glycine supplementation to low crude protein, amino acidsupplemented diets supports optimal performance of broiler chicks. Poult. Sci. 85:288-296.
  10. Furuya, S. 2008. An essential role for de novo biosynthesis of L-serine in CNS development. Asia Pac. J. Clin. Nutr. 17:312-315.
  11. Lamers, Y., J. Williamson, L. R. Gilbert, P. W. Stacpoole, and J. F. Gregory. 2007. Glycine turnover and decarboxylation rate quantified in healthy men and women using primed, constant infusions of [1,2-(13)C2]glycine and [(2)H3]leucine. J. Nutr. 137:2647-2652.
  12. Lee, C. W., Y. J. Oh, Y. S. Son, and W. G. An. 2011. Effects of dietary protein and threonine supply on in vitro liver threonine dehydrogenase activity and threonine efficiency in rat and chicken. Asian-Aust. J. Anim. Sci. 24:1417-1424.
  13. Le Floc'h, N., B. Seve, and Y. Henry. 1994. The addition of glutamic acid or protein to a threonine-deficient diet differentially affects growth performance and threonine dehydrogenase activity in fattening pigs. J. Nutr. 124:1987-1995.
  14. Le Floc'h, N., C. Obled, and B. Seve. 1996. In vivo threonine oxidation in growing pigs fed on diets with graded levels of threonine. Br. J. Nutr. 75:825-837.
  15. Liebert, F. 1995. Methodische untersuchungen zur beurteilung von lysinverwertungskennzahlen von schweinen nach extremen veranderungen von proteinmenge und -zusammensetzung. Arch. Anim. Nutr. 48:319-327.
  16. Liebert, F. 2008. Modelling of protein metabolism yields amino acid requirements dependent on dietary amino acid efficiency, growth response, genotype and age of growing chicken. Avian Biol. Res. 1:101-110.
  17. Moghaddam, H. S., H. N. Moghaddam, H. Kermanshahi, A. H. Mosavi, and A. Raji. 2011. The effect of threonine on mucin2 gene expression, intestinal histology and performance of broiler chicken. Ital. J. Anim. Sci. 10:e14.
  18. NRC. 1994. Nutrient requirements of poultry. 9th rev. ed. Natl. Acad. Press, Washington, DC.
  19. Osanai, M. and M. Okudaira. 2001. Syntheses of glycine and L-serine by their interconversion in the posterior silkgland of the silkworm, Bombyx mori. Amino Acids 20:113-121.
  20. Rimbach, M. and F. Liebert. 2000. Ergebnisse zum altersabhangigen Threoninbedarf aktueller Broilergenotypen. Proc. Soc. Nutr. Physiol. 9:106.
  21. Samadi and F. Liebert. 2006. Estimation of nitrogen maintenance requirement and potential for nitrogen deposition in fast growing chickens depending on age and sex. Poult. Sci. 85:1421-1429.
  22. Sartori, A., H. M. Garay-Malpartida, M. F. Forni, R. I. Schumacher, F. Dutra, M. C. Sogayar, and E. J. H. Bechara. 2008. Aminoacetone, a putative endogenous source of methylglyoxal, causes oxidative stress and death to insulin-producing RINm5f cells. Chem. Res. Toxicol. 21:1841-1850.
  23. Schutte, J. B., W. Smink, and M. Pack. 1997. Requirement of young broiler chicks for glycine and serine. Arch. Geflugelkd. 61:43-47.
  24. SPSS 2005. Statistical package for social sciences, SPSS 14.0 for Windows. Statistical package for social sciences. SPSS Inc., Chicago, IL, USA.
  25. te Braake, F., H. Schierbeek, K. de Groof, A. Vermes, M. Longini, G. Buonocore, and J. B. van Goudoever. 2008. Glutathione synthesis rates after amino acid administration directly after birth in preterm infants. Am. J. Clin. Nutr. 88:333-339.
  26. Thong, H. T. and F. Liebert. 2004. Potential for protein deposition and threonine requirement of modern genotype barrows fed graded levels of protein with threonine as limiting amino acid. J. Anim. Physiol. Anim. Nutr. (Berl) 88:196-203.
  27. Waguespack, A. M. 2005. Low crude protein, amino acid-supplemented diets, and the glycine requirement in low crude protein diets for broilers. A thesis. /available/etd-11142007-170943/unrestricted/AMWThesisabsolutefinalversion.pdf.
  28. Waguespack, A. M., S. Powell, T. D. Bidner, and L. L. Southern. 2009. The glycine plus serine requirement of broiler chicks fed low-crude protein, corn-soybean meal diets. J. Appl. Poult. Res. 18:761-765.
  29. Waldroup, P. W., Q. Jiang, and C. A. Fritts. 2005. Effects of glycine and threonine supplementation on performance of broiler chicks fed diets low in crude protein. Int. J. Poult. Sci. 4:250-257.
  30. Wecke, C. and F. Liebert. 2010. Optimal dietary lysine to threonine ratio in pigs (30-110 kg BW) derived from observed dietary amino acid efficiency. J. Anim. Physiol. Anim. Nutr. (Berl) 94:e277-285.
  31. Wyss, M. and R. Kaddurah-Daouk. 2000. Cretine and creatinine metabolism. Physiol. Rev. 8:1107-1213.
  32. Yuan, J. H. and R. E. Austic. 2001. The effect of dietary protein level on threonine dehydrogenase activity in chickens. Poult. Sci. 80:1353-1356.

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