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Development of Species-Specific PCR to Determine the Animal Raw Material

종 특이 프라이머를 이용한 동물성 식품원료의 진위 판별법 개발

  • Kim, Kyu-Heon (New Hazardous Substance Team, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety) ;
  • Lee, Ho-Yeon (New Hazardous Substance Team, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety) ;
  • Kim, Yong-Sang (New Hazardous Substance Team, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety) ;
  • Kim, Mi-Ra (New Hazardous Substance Team, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety) ;
  • Jung, Yoo Kyung (New Hazardous Substance Team, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety) ;
  • Lee, Jae-Hwang (New Hazardous Substance Team, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety) ;
  • Chang, Hye-Sook (New Hazardous Substance Team, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety) ;
  • Park, Yong-Chjun (Novel Food Division, Ministry of Food and Drug Safety) ;
  • Kim, Sang Yub (Hazardous Substances Analysis Team, Busan Regional Food and Drug Administration) ;
  • Choi, Jang Duck (New Hazardous Substance Team, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety) ;
  • Jang, Young-Mi (New Hazardous Substance Team, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety)
  • 김규헌 (식품의약품안전처 식품의약품안전평가원 신종유해물질팀) ;
  • 이호연 (식품의약품안전처 식품의약품안전평가원 신종유해물질팀) ;
  • 김용상 (식품의약품안전처 식품의약품안전평가원 신종유해물질팀) ;
  • 김미라 (식품의약품안전처 식품의약품안전평가원 신종유해물질팀) ;
  • 정유경 (식품의약품안전처 식품의약품안전평가원 신종유해물질팀) ;
  • 이재황 (식품의약품안전처 식품의약품안전평가원 신종유해물질팀) ;
  • 장혜숙 (식품의약품안전처 식품의약품안전평가원 신종유해물질팀) ;
  • 박용춘 (식품의약품안전처 신소재식품과) ;
  • 김상엽 (부산지방식품의약품안전청 유해물질분석팀) ;
  • 최장덕 (식품의약품안전처 식품의약품안전평가원 신종유해물질팀) ;
  • 장영미 (식품의약품안전처 식품의약품안전평가원 신종유해물질팀)
  • Received : 2014.06.28
  • Accepted : 2014.10.22
  • Published : 2014.12.31

Abstract

In this study, the detection method was developed using molecular biological technique to distinguish authenticity of animal raw materials. The genes for distinction of species about animals targeted at Cytochrome c oxidase subunit I (COI), Cytochrome b (Cytb), and 16S ribosomal RNA (16S rRNA) genes in mitochondrial DNA. The species-specific primers were designed by that Polymerase Chain Reaction (PCR) product size was around 200 bp for applying to processed products. The target 24 raw materials were 2 species of domestic animals, 6 species of poultry, 2 species of freshwater fishes, 13 species of marine fishes and 1 species of crustaceans. The results of PCR for Rabbit, Fox, Pheasant, Domestic Pigeon, Rufous Turtle Dove, Quail, Tree Sparrow, Barn Swallow, Catfish, Mandarin Fish, Flying Fish, Mallotus villosus, Pacific Herring, Sand Lance, Japanese Anchovy, Small Yellow Croaker, Halibut, Jacopever, Skate Ray, Ray, File Fish, Sea Bass, Sea Urchin, and Lobster raw materials were confirmed 113 bp ~ 218 bp, respectively. Also, non-specific PCR products were not detected in compare species by species-specific primers. The method using primers developed in this study may be applied to distinguish an authenticity of food materials included animal raw materials for various processed products.

Acknowledgement

Supported by : 식품의약품안전평가원

References

  1. Sawyer, J., Wood, C., Shanahan, D., Gout, S. and McDowell, D.: Real-time PCR for quantitative meat species, Food Cont., 14, 579-583 (2003). https://doi.org/10.1016/S0956-7135(02)00148-2
  2. Calvo, J.H., Zaragoza, P. and Pawlowski, R.: Validation of cytochrome b sequence analysis as method of species identification, J. Forensic Sci., 79, 2108-2212 (2001).
  3. Girish, P.S., Anjaneyulu, A.S.R., Viswas, K.N., Shivakumar, M., Anand, M., Patel, M. and Sharma, B.: Meat species identification by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) of mitochondrial 12S rRNA gene, Meat Sci., 70, 107-112 (2005). https://doi.org/10.1016/j.meatsci.2004.12.004
  4. Martin, I., Garcia, T., Fajardo, V., Lopez-calleja, I., Hernandez, P.E., Gonzalez, I. and Martin, R.: Species-specific pcr for the identification of ruminant species in feedstuffs, Meat Sci., 75, 120-127 (2007). https://doi.org/10.1016/j.meatsci.2006.06.019
  5. 정명생, 임경희: 활어의 소비구조 분석에 관한 연구, 한국해양수산개발원 (2003).
  6. Hacia, J.G.: Resequencing and mutational analysis using oligonucleotide microarrays, Nat Genet., 21(1 Supple), 42-47 (1999). https://doi.org/10.1038/4469
  7. Padovan, G.J., De Jong, D., Rodrigues, L.P. and Marchini J.S.: Detection of adulteration of commercial honey samples by the 13C/12C isotope ratio, Food Chem., 82, 633-636 (2003). https://doi.org/10.1016/S0308-8146(02)00504-6
  8. Schellenber, A., Chmielus, S., Schlicht, C., Camin, F., Perini, M., Bontempo, L., Heinrich, K., Kelly, S.D., Thomas, F., Jamin, E. and Horacek, M.: Multielement stable isotope ratio (H, C, N, S) of honey from different european regions, Food Chem., 121, 770-777(2010). https://doi.org/10.1016/j.foodchem.2009.12.082
  9. Hong E.J., Kim K.H., Par S.J., Kang J.W., Kim D.S., Lee H.J., Kim E.J., Lee J.W., Kim S.H., Lee K.H. and Noh B.S.: Discrimination of the salted cuttle fish and the salted mitra squid in economically motivatied authentication food using eletronic nose based on mass spectrometer, Food Eng. Progress., 15, 122-129 (2011).
  10. Hong E.J., Kim K.H., Par S.J., Kang J.W., Kim D.S., Lee H.J., Kim E.J., Lee J.H., Lee K.H. and Noh B.S.: Discrimination of shreds of frozen and dried alaska pollack, dried pollack and cod using electronic nose, Food Eng. Progress., 15, 162-168 (2011).
  11. Fabric, T., Celia, M. and Catherine, H.: Food and forensic molecular identification: update and challenges, TRENDS in Biotech., 23, 359-366 (2005). https://doi.org/10.1016/j.tibtech.2005.05.006
  12. Folmer, O., Black, M., Hoeh, W., Lutz, R. and Vrijenjoek, R.: DNA primers for amplication of mitochondrial cytochrome C oxidase subunit I from diverse metazoan invertebrates, Mol. Mar. Bio. and Biotech., 3, 294-299 (1994).
  13. Hird, H., Goodier, R. and Hill, M.: Rapid detection of chicken and turkey in heated meat products using the polymerase chain reaction followed by amplicon visualization with vistra green, Meat Sci., 65, 1117-1123 (2003). https://doi.org/10.1016/S0309-1740(02)00341-8
  14. Arslan, A., Irfan Ilhak, O. and Calicioglu, M.: Effect of method of cooking on identification of heat processed beef using polymerase chain reaction (PCR) technique, Meat Sci., 72, 326-330 (2006). https://doi.org/10.1016/j.meatsci.2005.08.001
  15. Hebert P.D., Cywinska, A., Ball, S.L., and deWaard, J.R.: Biological identifications through DNA Barcodes, Pro. R. Soc. Lond. B., 270, 313-321 (2003). https://doi.org/10.1098/rspb.2002.2218
  16. Michael T.M., Michael B., Ryan Gregory, T. and Alfried P.V.: DNA-based species delineation in tropical beetles using mitochondrial and nuclear markers, Philosophical Transactions of The Royal Society B., 360, 1925-1933 (2005). https://doi.org/10.1098/rstb.2005.1724
  17. Tomoaki, M., Atsushi, A., Takuma, T., Sumitaka, Y., Yutaka, O. and Manabu, Y.: Identification of animal species using the partial sequences in the mitochondrial 16S rRNA Gene, Legal Med., 11, 449-450 (2009). https://doi.org/10.1016/j.legalmed.2009.02.002
  18. Shin S.C., Chung K.Y. and Chung E.R.: Identification of meat species using PCR-RFLP marker of cytochrome b gene, Korean J. Food Sci. Ani. Resour., 26, 375-379 (2006).
  19. Park Y.K., Ahn C.Y., Jin S.O., Lim J.Y., Kim K.H., Lee J.H., Cho T.Y., Lee H.J., Park K.S. and Yoon H.S.: Identification of raw materials in processed meat products by PCR using species-specific primer, J. Fd Hyg. Safety., 27, 68-73 (2012). https://doi.org/10.13103/JFHS.2012.27.1.068
  20. Knuutinen, J. and Harjula, P.: Identification of fish species by reversed-phase high-performance liquid chromatography with photodiode-array detection, J. Chromatogr., 705, 11-21 (1998). https://doi.org/10.1016/S0378-4347(97)00505-7
  21. Hird, H., Goodier, R. and Hill, M.: Rapid detection of chicken and turkey in heated meat products using the polymerase chain reaction followed by amplification visualization with vistra green, Meat Sci., 65, 1117-1123 (2003). https://doi.org/10.1016/S0309-1740(02)00341-8
  22. Park J.K., Shin K.H., Chung K.Y. and Chung E.R.: Identification of meat species using species-specific PCR-RFLP Fingerprint of mitochondrial 12S rRNA gene, Korean J. Food Sci. Ani. Resour., 27, 209-215 (2007). https://doi.org/10.5851/kosfa.2007.27.2.209
  23. Kim M.Y., Kim J.H., Kim H.J., Park S.H., Woo G.J. and Kim H.Y.: Monitoring of genetically modified soybean and processed food in korean market using PCR, J. Korean Soc. Agric. Chem. Biotechnol., 46, 344-347 (2003).
  24. Jeon Y.J., Kang E.S. and Hong K.W.: A PCR method for rapid detection of buckwheat ingredients in food, J. Korean Soc. Agric. Chem. Biotechnol., 17, 335-341 (2007).
  25. Branicki, W., Kupiec, T. and Pawlowski, R.: Validation of cytochrome b sequence analysis as method of species identification, J. Forensic Sci., 48, 83-87 (2003).
  26. Rodriquez, M.A., Garcia, T., Gonzalez, I., Asensio, L., Mayoral, B., Lopez-Calleja, I., Hernandez, P.E. and Martin, R.: TaqMan real-time PCR for the detection and quantitation of pork in meat mixtures, Meat Sci., 70, 113-120 (2005). https://doi.org/10.1016/j.meatsci.2004.12.005
  27. Hsieh, H.M., Chiang, H.L., Tsai, L.C., Lai, S.Y., Huang, N.E., Linacre, A. and Lee, J.C.: Cytochrome b gene for species identification of the conservation animals, Forensic. Sci. Int., 122, 7-18 (2001). https://doi.org/10.1016/S0379-0738(01)00403-0
  28. Wolstenholme, D.R.: Animal mitochondreal DNA: structure and evolution, Int. Rev. Cytol, 141, 173-216 (1992). https://doi.org/10.1016/S0074-7696(08)62066-5
  29. Sunnucks, P.: Efficient genetic markers for population biology, Trends Ecol Evol., 15(5), 199-203 (2000). https://doi.org/10.1016/S0169-5347(00)01825-5

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