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Bacterial diversity in children's dental caries

소아의 치아 우식 부위별 세균 다양성

  • Kim, Eun-Mi (Department of Dental Hygiene, Gwangju Health University) ;
  • Baik, Keun-Sik (Division of Life Science, Korea Basic Science Institute) ;
  • Ha, Myung-Ok (Department of Dental Hygiene, Gwangju Health University)
  • 김은미 (광주보건대학교 치위생과) ;
  • 백근식 (한국기초과학지원연구원 생명과학연구부) ;
  • 하명옥 (광주보건대학교 치위생과)
  • Received : 2013.07.31
  • Accepted : 2013.10.17
  • Published : 2013.10.31

Abstract

Objectives : Molecular biology techniques were employed to assess diversity of bacterial in children's dental caries. Methods : DNA of germs was extracted and the diversity of the 16S rRNA clones was analyzed by amplified rDNA restriction analysis and sequencing. The experimental samples were pit and fissure caries (PC), deep dentinal caries (DC), smooth surface caries (SC), and supragingival plaque (PQ) from 50 children of age less than 12 years old. The control group was healthy teeth supragingival plaque (HT). Thirty clones from each 16S rRNA clone library of 5 samples were randomly selected, thus a total of 150 clones were analyzed. Results : Amplified rDNA restriction analysis uncovered 18, 20, 11, 17, and 22 phylotypes from healthy teeth, pit and fissure caries, deep dentinal caries, smooth surface caries, and supragingival plaque, respectively. Sequencing analysis found the dominance of Actinomycs naeslundii and Fusobacterium nucleatum in the healthy teeth; Leptotrichia sp. in the pit and fissure caries; Actinomyces sp., Streptococcus mutans, and Rahnella aquatilis in the deep dentinal caries; Streptococcus mutans and Actinomyces sp. in the smooth surface caries; Enterobacter hormaechei and Streptococcus sanguinis in the supragingival plaque. Conclusions : Clonal analysis identified 6 phyla, 20 genera, and 51 species.

Keywords

ARDRA;bacterial diversity;dental caires;16S rRNA gene

Acknowledgement

Supported by : 광주보건대학교

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