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Application of Embryo Transfer Technology

수정란 이식 기술의 응용

  • 임현주 (농촌진흥청 국립축산과학원) ;
  • 손준규 (농촌진흥청 국립축산과학원) ;
  • 윤호백 (농촌진흥청 국립축산과학원) ;
  • 백광수 (농촌진흥청 국립축산과학원) ;
  • 최창용 (농촌진흥청 국립축산과학원) ;
  • 김시동 (농촌진흥청 국립축산과학원) ;
  • 권응기 (농촌진흥청 국립축산과학원)
  • Received : 2013.08.22
  • Accepted : 2013.09.11
  • Published : 2013.09.30

Abstract

Embryo transfer (ET) technology is of high importance in modern cattle breeding programs. ET is one step in the process of removing one or more embryos from the reproductive tract of an outstanding donor female and transferring them to one or more recipient females. Embryos also can be produced in the laboratory via techniques such as in vitro fertilization (IVF). But the actual transfer of an embryo is only one step in a series of processes that may include some or all of the following: superovulation and insemination of donors, collection of embryos, isolation, evaluation and short-term storage of embryos, micromanipulation and genetic testing of embryos, freezing of embryos and embryo transfer. Cryopreservation and direct transfer of frozen-thawed embryos is common-place with pregnancy rates near that of fresh embryos. Polymerase chain reaction (PCR) technology is currently being used for sexing embryos, and this technology will be used for "embryo diagnostics" and "embryo genomics" in the future. Although, many limitations and problems remain to overcome, these and other new technologies promise to change livestock breeding drastically in the next decade.

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