The Optimal Combination of the Mixture of Unripe Rubus coreanus and Astragalus membranaceus in the Activation and Differentiation of Osteoblastic Cells

복분자 미성숙과와 황기 추출물 혼합비율에 의한 조골세포의 활성연구

  • Received : 2013.10.17
  • Accepted : 2013.10.24
  • Published : 2013.10.31


Rubus coreanus and Astragalus membranaceus Bunge have been used as a traditional medicine with various pharmacological properties for a long time in Asia. We investigated the proliferative and differentiative effects on osteoblastic cells, MG-63 in various mixture ratios of immature fructus extracts of R. coreanus and root extracts of A. membranaceus. With preliminary in vivo ovariectomized mice, we confirmed the effects of the supplementation of various mixture on alkaline phosphatase (ALP) activity in serum. The ratio of 7:3 is considered as the most effective ratio for the proliferation of osteoblastic cells as they increased by 56.8% similar to estradiol. ALP activity in osteoblast increased by the combination of R. coreanus and A. membranaceus and peaked in the ratio of 72:28 and 68:32 respectively. These results indicate that the optimal combination of R. coreanus and A. membranaceus in 7:3 has the most beneficial effects on the activation of osteoblast cells, and give further possibilities that it can be used clinically in the treatment of osteoporosis.


Alkaline phosphatase;Astragalus membranaceus;MG-63;Osteocalcin;Rubus coreanus


  1. Choi, C., H. Lee, H. Lim, S. Park, J. Lee and S. Do. 2012. Effect of Rubus coreanus extracts on diabetic osteoporosis by simultaneous regulation of osteoblasts and osteoclasts. Menopause 19:1043-1051.
  2. Ilich, J.Z. and J.E. Kerstetter. 2000. Nutrition in bone health revisited: a story beyond calcium. J. Am. Coll. Nutr. 19:715-737.
  3. Jeon, Y.H., S.W. Choi and M.R. Kim. 2009. Antimutagenic and cytotoxic activity of ethanol and water extracts from Rubus coreanus. Korean J. Food Cookery Sci. 25:379-386.
  4. Kang, S.C., J.D. Lim, J.C. Lee, H.J. Park, N.S. Kang and E.H. Sohn. 2010. Effects of fructus and semen from Rosa rugosa on Osteoimmune cells. Korean J. Plant Res. 4:157-164.
  5. Karsenty, G. 2003. The complexities of skeletal biology. Nature 423:316-318.
  6. Kennel, K.A. and M.T. Drake. 2009. Adverse effects of bisphosphonates: Implications for Osteoporosis management. Mayo. Clin. Proc. 84:632-638.
  7. Kim, S., C.K. Kim, K.S. Lee, J.H. Kim, H. Hwang, D. Jeoung, J. Choe, M.H. Won, H. Lee, K.S. Ha, Y.G. Kwon and Y.M. Kim. 2013. Aqueous extract of unripe Rubus coreanus fruit attenuates atherosclerosis by improving blood lipid profile and inhibiting NF-$\kappa{B}$ activation via phase II gene expression. J. Ethnopharmacol. 146:515-524.
  8. Komori, T. 2010. Regulation of osteoblast differentiation by Runx2. Adv. Exp. Med. Biol. 658:43-49.
  9. Lee, K.H. and E.M. Choi. 2006. Rubus coreanus Miq. extract promotes osteoblast differentiation and inhibits bone-resorbing mediators in MC3T3-E1 cells. Am. J. Chin. Med. 34:643-654.
  10. Lu, S., K.J. Chen, Q.Y. Yang and H.R. Sun. 2011. Progress in the research of Radix Astragali in treating chronic heart failure: effective ingredients, dose-effect relationship and adverse reaction. Chin. J. Int. Med. 17:473-477.
  11. Manglagas, S.C. 2000. Birth and death of bone cells: basic regulatory mechanisms and implications for the pathogenesis and treatment of osteoporosis. Endocr. Rev. 21:115-137
  12. Ochiuto, F., R.D. Pasquale, G. Gualielmo, D.R. Palumbo, G. Zangla, S. Samperi, A. Renzo and C. Circosta. 2007. Effects of phytoestrogenic isoflavones from red clover (Trifolium pretense L.) on experimental osteoporosis. Phytother. Res. 21:130-134.
  13. Park, M.C., K.J. Kim, H.S. Lee and E.H. Jo. 2007. Attenuation of airway hyperreactivity (AHR) and inflammation by water extract of Rubus coreanus Miq. (WRCM). J. Korean Orient. Med. Ophthalmol. Otolaryngol. Dermato. 20:177-194.
  14. Pertynski, T. and G. Stachowiak. 2006. Menopause-facts and controversies. Endokrynol. Pol. 57:525-534.
  15. Seibel, M.J. 2005. Biochemical markers of bone turnover:part I:biochemistry and variabillity. Clin. Biochem. Rev. 26:97-122.

Cited by

  1. Effects of Rubus coreanus-Cheonggukjang on Bone Mineral Density and Bone Mineral Content in Growing Rats vol.20, pp.4, 2015,
  2. Effect of water extract and distillate from the mixture of black goat meat and medicinal herb on osteoblast proliferation and osteoclast formation vol.48, pp.2, 2015,
  3. Anti-osteoporotic Effects of Unripe Fructus of Rubus coreanus Miquel in Osteoblastic and Osteoclastic Cells vol.27, pp.6, 2014,
  4. Effect of the combinatory mixture of Rubus coreanus Miquel and Astragalus membranaceus Bunge extracts on ovariectomy-induced osteoporosis in mice and anti-RANK signaling effect vol.151, pp.2, 2014,


Supported by : 농촌진흥청