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The Optimal Combination of the Mixture of Unripe Rubus coreanus and Astragalus membranaceus in the Activation and Differentiation of Osteoblastic Cells

복분자 미성숙과와 황기 추출물 혼합비율에 의한 조골세포의 활성연구

  • Received : 2013.10.17
  • Accepted : 2013.10.24
  • Published : 2013.10.31

Abstract

Rubus coreanus and Astragalus membranaceus Bunge have been used as a traditional medicine with various pharmacological properties for a long time in Asia. We investigated the proliferative and differentiative effects on osteoblastic cells, MG-63 in various mixture ratios of immature fructus extracts of R. coreanus and root extracts of A. membranaceus. With preliminary in vivo ovariectomized mice, we confirmed the effects of the supplementation of various mixture on alkaline phosphatase (ALP) activity in serum. The ratio of 7:3 is considered as the most effective ratio for the proliferation of osteoblastic cells as they increased by 56.8% similar to estradiol. ALP activity in osteoblast increased by the combination of R. coreanus and A. membranaceus and peaked in the ratio of 72:28 and 68:32 respectively. These results indicate that the optimal combination of R. coreanus and A. membranaceus in 7:3 has the most beneficial effects on the activation of osteoblast cells, and give further possibilities that it can be used clinically in the treatment of osteoporosis.

Keywords

Alkaline phosphatase;Astragalus membranaceus;MG-63;Osteocalcin;Rubus coreanus

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  1. Effects of Rubus coreanus-Cheonggukjang on Bone Mineral Density and Bone Mineral Content in Growing Rats vol.20, pp.4, 2015, https://doi.org/10.3746/pnf.2015.20.4.260
  2. Effect of water extract and distillate from the mixture of black goat meat and medicinal herb on osteoblast proliferation and osteoclast formation vol.48, pp.2, 2015, https://doi.org/10.4163/jnh.2015.48.2.157
  3. Anti-osteoporotic Effects of Unripe Fructus of Rubus coreanus Miquel in Osteoblastic and Osteoclastic Cells vol.27, pp.6, 2014, https://doi.org/10.7732/kjpr.2014.27.6.593
  4. Effect of the combinatory mixture of Rubus coreanus Miquel and Astragalus membranaceus Bunge extracts on ovariectomy-induced osteoporosis in mice and anti-RANK signaling effect vol.151, pp.2, 2014, https://doi.org/10.1016/j.jep.2013.12.008

Acknowledgement

Supported by : 농촌진흥청