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Identification and Association of SNPs in TBC1D1 Gene with Growth Traits in Two Rabbit Breeds

  • Yang, Zhi-Juan ;
  • Fu, Lu ;
  • Zhang, Gong-Wei ;
  • Yang, Yu ;
  • Chen, Shi-Yi ;
  • Wang, Jie ;
  • Lai, Song-Jia
  • Received : 2013.05.17
  • Accepted : 2013.07.18
  • Published : 2013.11.01

Abstract

The TBC1D1 plays a key role in body energy homeostasis by regulating the insulin-stimulated glucose uptake in skeletal muscle. The present study aimed to identify the association between genetic polymorphisms of TBC1D1 and body weight (BW) in rabbits. Among the total of 12 SNPs detected in all 20 exons, only one SNP was non-synonymous (c.214G>A. p.G72R) located in exon 1. c.214G>A was subsequently genotyped among 491 individuals from two rabbit breeds by the high-resolution melting method. Allele A was the predominant allele with frequencies of 0.7780 and 0.6678 in European white rabbit (EWR, n = 205) and New Zealand White rabbit (NZW, n = 286), respectively. The moderate polymorphism information content (0.250.05). Our results implied that the c.214G>A of TBC1D1 gene might be one of the candidate loci affecting the trait of 35 d BW in the rabbit.

Keywords

TBC1D1;Polymorphism;High-resolution Melting;Growth Traits;Rabbit

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