Association of Tyrosinase (TYR) and Tyrosinase-related Protein 1 (TYRP1) with Melanic Plumage Color in Korean Quails (Coturnix coturnix)

  • Xu, Ying (College of Animal Science, Henan University of Science and Technology) ;
  • Zhang, Xiao-Hui (College of Animal Science, Henan University of Science and Technology) ;
  • Pang, You-Zhi (College of Animal Science, Henan University of Science and Technology)
  • Received : 2013.03.20
  • Accepted : 2013.05.30
  • Published : 2013.11.01


TYR (Tyrosinase) and TYRP1 (Tyrosinase-related protein 1) play crucial roles in determining the coat color of birds. In this paper, we aimed to characterize the relationship of TYR and TYRP1 genes with plumage colors in Korean quails. The SNPs were searched by cDNA sequencing and PCR-SSCP in three plumage color Korean quails (maroon, white and black plumage). Two SNPs ($367T{\rightarrow}C$ and $1153C{\rightarrow}T$) were found in the coding region of TYRP1 gene, but had no significant association with plumage phenotype in Korean quails. The expression of TYR was higher in black plumage quails than that in maroon plumage quails. In contrast, the expression of TYRP1 was lower in black plumage quails than that in maroon plumage quails. This study suggested that the melanic plumage color in Korean quails may be associated with either increased production of TYR or decreased production of TYRP1.


Korean Quail;TYR;TYRP1;Polymorphism;Gene Expression


  1. Dame, M. C. F., G. M. Xavier, J. P. Oliveira-Filho, A. S. Borges, H. N. Oliveira, F. Riet-Correa, and A. L. Schild. 2012. A nonsense mutation in the tyrosinase gene causes albinism in water buffalo. BMC Genet. 13:62.
  2. Ghanem, G. and J. Fabrice. 2011. Tyrosinase related protein 1 (TYRP1/gp75) in human cutaneous melanoma. Mol. Oncol. 5: 150-155.
  3. Gratten, J., D. Beraldi, B. V. Lowder, A. F. McRae, P. M. Visscher, J. M. Pemberton, and J. Slate. 2007. Compelling evidence that a single nucleotide substitution in TYRP1 is responsible for coat-colour polymorphism in a free-living population of Soay sheep. Proc. R. Soc. B. 274:619-626.
  4. Guibert, S., M. Girardot, H. Leveziel, R. Julien, and A. Oulmouden. 2004. Pheomelanin coat colour dilution in French cattle breeds is not correlated with the TYR, TYRP1 and DCT transcription levels. Pigment Cell Res. 17:337-345.
  5. Huang, Y. H., T. H. Lee, K. J. Chan, F. L. Hsu, Y. C. Wu, and M. H. Lee. 2008. Anemonin is a natural bioactive compound that can regulate tyrosinase-related proteins and mRNA in human melanocytes. J. Dermatol. Sci. 49:115-123.
  6. Imes, D. L., L. A. Geary, R. A. Grahn, and L. A. Lyons. 2006. Albinism in the domestic cat (Felis catus) is associated with a tyrosinase (TYR) mutation. Anim. Genet. 37:175-178.
  7. Ito, S. 2006. Encapsulation of a reactive core in neuromelanin. Proc. Natl. Acad. Sci. USA. 103:14647-14648.
  8. Kobayashi, T., G. Imokawa, D. C. Bennett, and V. J. Hearing. 1998. Tyrosinase stabilization by TYRP1 (the brown locus protein). J. Biol. Chem. 273:31801-31805.
  9. Li, S., C. Wang, W. Yu, S. Zhao, and Y. Gong. 2012. Identification of genes related to white and black plumage formation by RNA-Seq from white and black feather bulbs in ducks. Plos. One. 7:e36592.
  10. Mondal, M., M. Sengupta, S. Samanta, A. Sil, and K. Ray. 2012. Molecular basis of albinism in India: Evaluation of seven potential candidate genes and some new findings. Gene 511: 470-474.
  11. Murisier, F., S. Guichard, and F. Beermann. 2006. A conserved transcriptional enhancer that specifies TYRP1 expression to melanocytes. Dev. Biol. 298:644-655.
  12. Nadeau, N. J., N. I. Mundy, D. Gourichon, and F. Minvielle. 2007. Association of a single-nucleotide substitution in TYRP1 with roux in Japanese quail (Coturnix japonica). Anim. Genet. 38: 609-613.
  13. Nan, H., P. Kraft, D. J. Hunter, and J. Han. 2009. Genetic variants in pigmentation genes, pigmentary phenotypes, and risk of skin cancer in Caucasians. Int. J. Cancer 125:909-917.
  14. Okomo-Adhiambo, M., A. Rink, W. M. Rauw, and L. Gomez-Raya. 2012. Gene expression in Sinclair swine with malignant melanoma. Animal 6:179-192.
  15. Olivares, C. and F. Solano. 2009. New insights into the active site structure and catalytic mechanism of tyrosinase and its related proteins. Pigment Cell. Melanoma. Res. 22:750-760.
  16. Oribe, E., A. Fukao, C. Yoshihara, M. Mendori, K. G. Rosal, S. Takahashi, and S. Takeuchi. 2012. Conserved distal promoter of the agouti signaling protein (ASIP) gene controls sexual dichromatism in chickens. Gen. Comp. Endocrinol. 177:231-237.
  17. Rad, H. H., T. Yamashita, H. Y. Jin, K. Hirosaki, K. Wakamatsu, S. Ito, and K. Jimbow. 2004. Tyrosinase-related proteins suppress tyrosinase-mediated cell death of melanocytes and melanoma cells. Exp. Cell Res. 298:317-328.
  18. Randhawa, M., T. Huff, J. C. Valencia, Z. Younossi, V. Chandhoke, V. J. Hearing, and A. Baranova. 2009. Evidence for the ectopic synthesis of melanin in human adipose tissue. FASEB. J. 23: 835-843.
  19. Ray, K., M. Chaki, and M. Sengupta, 2007. Tyrosinase and ocular diseases: Some novel thoughts on the molecular basis of oculocutaneous albinism type 1. Prog. Retin. Eye Res. 26:323-358.
  20. Ren, J., H. Mao, Z. Zhang, S. Xiao, N. Ding, and L. Huang. 2011. A 6-bp deletion in the TYRP1 gene causes the brown colouration phenotype in Chinese indigenous pigs. Heredity 106:862-868.
  21. Schmutz, S. M., T. G. Berryere, and A. D. Goldfinch, 2002. TYRP1 and MC1R genotypes and their effects on coat color in dogs. Mamm. Genome 13:380-387.
  22. Sturm, R. A., R. D. Teasdale, and N. F. Box. 2001. Human pigmentation genes: identification, structure and consequences of polymorphic variation. Gene 277:49-62.
  23. Tully, G. 2007. Genotype versus phenotype: Human pigmentation. Genetics 1:105-110.
  24. Wang, P., Y. Li, W. Hong, J. Zhen, J. Ren, Z. Li, and A. Xu, 2012. The changes of microRNA expression profiles and tyrosinase related proteins in MITF knocked down melanocytes. Mol. Biosyst. 8:2924-2931.
  25. Yu, J. S. and A. K. Kim. 2010. Effect of combination of taurine and azelaic on antimelanogenesis in murine melanoma cells. J. Biomed. Sci. 17:S45.

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