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Autophagy Inhibition Sensitizes Cisplatin Cytotoxicity in Human Gastric Cancer Cell Line Sgc7901

  • Zhang, Hui-Qing (The 3rd department of Medical Oncology, Jiangxi Provincial Tumor Hospital) ;
  • He, Bo (The 3rd department of Medical Oncology, Jiangxi Provincial Tumor Hospital) ;
  • Fang, Nian (Gastrointestinal Department of Internal Medicine, the 4th Affiliated Hospital of Nanchang University) ;
  • Lu, Shan (The 3rd department of Medical Oncology, Jiangxi Provincial Tumor Hospital) ;
  • Liao, Yu-Qian (The 3rd department of Medical Oncology, Jiangxi Provincial Tumor Hospital) ;
  • Wan, Yi-Ye (The 3rd department of Medical Oncology, Jiangxi Provincial Tumor Hospital)
  • Published : 2013.08.30

Abstract

We aimed to investigate the mechanism and effects of autophagy on cisplatin (DDP)-induced apoptosis in human gastric cancer cell line SGC7901. After SGC7901 cells were treated with DDP and/or chloroquine, cell proliferation was measured using MTT assay; cell apoptosis was determined by flow cytometry; autophagy and apotosis-related proteins expression were detected by Western blot; and quantitative analysis of autophagy after monodansylcadaverine (MDC) staining was performed using fluorescence microscopy. We found after treatment with 5 mg/L DDP for 24 h, the rates of cell apoptosis were ($21.07{\pm}2.12$)%. Autophagy, characterized by an increase in the number of autophagic vesicles and the level of LC3-II protein was observed in cells treated with DDP. After inhibition of autophagy by chloroquine, the rates of cell apoptosis were increased to ($30.16{\pm}3.54$)%, and the level of Caspase-3 and P53 protein were increased, and Bcl-2 protein was decreased. Therefore, autophagy protects human gastric cancer cell line SGC7901 against DDP-induced apoptosis, inhibition of autophagy can promote apoptosis, and combination therapy with DDP and chloroquine may be a promising therapeutic strategy for gastric cancer.

Keywords

Autophagy;cisplatin;chloroquine;apoptosis;gastric cancer

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