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Effect of miR27a on Proliferation and Invasion in Colonic Cancer Cells

  • Gao, Yang (Department of General Surgery, Henan Cancer Hospital) ;
  • Li, Bao-Dong (Department of General Surgery, Henan Cancer Hospital) ;
  • Liu, Yong-Gang (Department of General Surgery, Henan Cancer Hospital)
  • Published : 2013.08.30

Abstract

The aim of this study was to detect the expression of miR196a, miR146a, miR27a and miR200a in patients with colon cancer, and investigate the effect of miR27a expression on proliferation and invasion in colonic cancer cells. RT-PCR was employed to detect the expression levels in colon cancers. Then, colon cancer cells were cultured and transfected with 100 nM of miR27a mimics (80 nmol/L) or 80 nM miR27a inhibitors (80 nmol/L) in 24-well plates. Proliferation and invasion of colonic cancer cells were then determined by CCK-8 and Transwell assays, respectively. Our data showed miR27a to be high-expressed in patients with colon cancer. In addition, proliferation and invasion in the miR27a mimic group were significantly higher than in the control group and negative group (P<0.05), while, proliferation and invasion in the miR27a inhibitor group were obviously lowered (P<0.05). In conclusion, high expression of miR27a may play an important role in enhancing proliferation and invasion of colon cancer cells.

Keywords

Colon cancer cells;miR27a;proliferation;invasion

References

  1. Bullock MD, Pickard KM, Nielsen BS, et al (2013). Pleiotropic actions of miR-21 highlight the critical role of deregulated stromal microRNAs during colorectal cancer progression. Cell Death Dis, 4, e684. https://doi.org/10.1038/cddis.2013.213
  2. Fendler A, Jung K (2013). MicroRNAs as new diagnostic and prognostic biomarkers in urological tumors. Crit Rev Oncog, 18, 289-302. https://doi.org/10.1615/CritRevOncog.2013007176
  3. Filipowicz W, Bhattacharyya SN, Sonenberg N (2008). Mechanisms of post-transcriptional regulation by microRNAs: are the answers in sight? Nat Rev Genet, 9, 102-14.
  4. Gargalionis AN, Basdra EK (2013). Insights in microRNAs Biology. Curr Top Med Chem, 13, 1493-502. https://doi.org/10.2174/15680266113139990098
  5. Kahlert C, Kalluri R (2013). Exosomes in tumor microenvironment influence cancer progression and metastasis. J Mol Med (Berl), 91, 431-7. https://doi.org/10.1007/s00109-013-1020-6
  6. Kong X, Du Y, Wang G, et al (2011). Detection of differentially expressed microRNAs in serum of pancreatic ductal adenocarcinoma patients: miR-196a could be a potential marker for poor prognosis. Dig Dis Sci, 56, 602-9. https://doi.org/10.1007/s10620-010-1285-3
  7. Lerner M, Lundgren J, Akhoondi S, et al (2011). MiRNA-27a controls FBW7/hCDC4-dependent cyclin E degradation and cell cycle progression. Cell Cycle, 10, 2172-83. https://doi.org/10.4161/cc.10.13.16248
  8. Li Z, Hu S, Wang J, et al (2010). MiR-27a modulates MDR1/Pglycoprotein expression by targeting HIPK2 in human ovarian cancer cells. Gynecol Oncol, 119, 125-30. https://doi.org/10.1016/j.ygyno.2010.06.004
  9. Li Y, Li W, Ouyang Q, et al (2011). Detection of lung cancer with blood microRNA-21 expression levels in Chinese population. Oncol Lett, 2, 991-4.
  10. Li M, Marin-Muller C, Bharadwaj U, et al (2009). MicroRNAs: control and loss of control in human physiology and disease. World J Surg, 33, 667-84. https://doi.org/10.1007/s00268-008-9836-x
  11. Liu D, Sun Q, Liang S, et al (2013). MicroRNA-27a inhibitors alone or in combination with perifosine suppress the growth of gastric cancer cells. Mol Med Rep, 7, 642-8.
  12. Liu T, Tang H, Lang Y, et al (2009). MicroRNA-27a functions as an oncogene in gastric adenocarcinoma by targeting prohibitin. Cancer Lett, 273, 233-42. https://doi.org/10.1016/j.canlet.2008.08.003
  13. Lucas K, Raikhel AS (2013). Insect microRNAs: biogenesis, expression profiling and biological functions. Insect Biochem Mol Biol, 43, 24-38. https://doi.org/10.1016/j.ibmb.2012.10.009
  14. Mertens-Talcott SU, Chintharlapalli S, Li X, et al (2007). The oncogenic microRNA-27a targets genes that regulate specificity protein transcription factors and the G2-M checkpoint in MDA-MB-231 breast cancer cells. Cancer Res, 67, 11001-11. https://doi.org/10.1158/0008-5472.CAN-07-2416
  15. Mohammed Abba, Heike Allgayer (2009). MicroRNAs as regulatory molecules in cancer: a focus on models defining miRNA functions. Drug Discov Today Dis Model, 6, 13-9. https://doi.org/10.1016/j.ddmod.2009.01.002
  16. Okayama H, Schetter AJ, Harris CC (2012). MicroRNAs and inflammation in the pathogenesis and progression of colon cancer. Dig Dis, 30, 9-15. https://doi.org/10.1159/000341882
  17. Rothschild SI (2013). Epigenetic Therapy in Lung Cancer - Role of microRNAs. Front Oncol, 3, 158.
  18. Samimi H, Zaki Dizaji M, Ghadami M, et al (2013). MicroRNAs networks in thyroid cancers: focus on miRNAs related to the fascin. J Diabetes Metab Disord, 12, 31. https://doi.org/10.1186/2251-6581-12-31
  19. Schepeler T, Reinert JT, Ostenfeld MS, et al (2008). Diagnostic and prognostic microRNAs in stage II colon cancer. Cancer Res, 68, 6416-24. https://doi.org/10.1158/0008-5472.CAN-07-6110
  20. Schultz NA, Andersen KK, Roslind A, et al (2012). Prognostic microRNAs in cancer tissue from patients operated for pancreatic cancer--five microRNAs in a prognostic index. World J Surg, 36, 2699-707. https://doi.org/10.1007/s00268-012-1705-y
  21. Wang X, Tang S, Le SY, et al (2008). Aberrant expression of oncogenic and tumor-suppressive microRNAs in cervical cancer is required for cancer cell growth. PLoS One, 3, e2557. https://doi.org/10.1371/journal.pone.0002557
  22. Yan Z, Li J, Xiong Y, et al (2012). Identification of candidate colon cancer biomarkers by applying a random forest approach on microarray data. Oncol Rep, 28, 1036-42.
  23. Zhang J, Guo H, Qian G, et al (2010). Mir-145, a new regulator of the DNA fragmentation factor-45 (DFF45)-mediated apoptotic networkr. Mol Cancer, 9, 211. https://doi.org/10.1186/1476-4598-9-211
  24. Zhang H, Li M, Han Y, et al (2010). Down-regulation of miR-27a might reverse multidrug resistance of esophageal squamous cell carcinoma. Dig Dis Sci, 55, 2545-51. https://doi.org/10.1007/s10620-009-1051-6
  25. Zhao L, Bode AM, Cao Y, et al (2012). Regulatory mechanisms and clinical perspectives of miRNA in tumor radiosensitivity. Carcinogenesis, 33, 2220-7. https://doi.org/10.1093/carcin/bgs235

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