Variation of Blood T Lymphocyte Subgroups in Patients with Non- small Cell Lung Cancer

  • Wang, Wen-Jing (Department of Infectious Diseases, Nanjing First Hospital, Nanjing Medical University) ;
  • Tao, Zhen (Department of Infectious Diseases, Nanjing First Hospital, Nanjing Medical University) ;
  • Gu, Wei (Department of Respiration, Nanjing First Hospital, Nanjing Medical University) ;
  • Sun, Li-Hua (Department of Respiration, Nanjing First Hospital, Nanjing Medical University)
  • Published : 2013.08.30


Objectives: To study variation in T lymphocyte subgoups and its clinical significance in non-small cell lung cancer (NSCLC). Methods: Levels of CD3+, CD4+, CD8+, CD4+/CD8+, NK and Treg cells in peripheral blood of NSCLC cases and healthy adults were determined by flow cytometry. Results: CD3+, CD4+ and CD4+/CD8+ ratio and NK cells in NSCLCs were decreased significantly in comparison with the control group (P < 0.01), and decreased with increase in the clinical stage of NSCLC, while CD8+ cells demonstrated no significant change (P > 0.05). Treg cells were significantly more frequent than in the control group (P < 0.01), and increased with the clinical stage of NSCLC. Conclusion: The cellular immune function of the NSCLC patients is lowered. It is important to detect change of T lymphocyte subgroups by flow cytometry for the diagnosis, treatment and prognostic assessment of NSCLC patients.


Non-small cell lung cancer;lymphocyte subset;flow cytometry;cellular immune


  1. Dou X, Wang RB, Yan HJ, et al (2013). Circulating lymphocytes as predictors of sensitivity to preoperative chemoradiotherapy in rectal cancer cases. Asian Pac J Cancer Prev, 14, 3881-5.
  2. Erdem MG, Cinkilic N, Vatan O, et al (2012). Genotoxic and anti-genotoxic effects of vanillic acid against mitomycin C-induced genomic damage in human lymphocytes in vitro.Asian Pac J Cancer Prev, 13, 4993-8.
  3. Hakansson A, Hakansson L, Gustaf sson B, et al (2003). On the effect of biochemotherapy in metastatic malignant melanoma: an unopathological evaluation. Melanoma Res, 13, 401-8.
  4. Hanaki K, Momo A, Oku T, et al (2003). Semiconductor quantum dot/albumin complex is a long-life and highly photostable endosome marker. Biochem Biophys Res Commun, 302, 496-501.
  5. Jason D, Marc A, Alexander Y, et al (2003). Foxp3 programs the development and function of CD4+ CD25+ regulatory T cells. Nature Immuno, 4, 330-6.
  6. Karaman H, Karaman A, Erden A, et al (2013). Relationship between colonic polyp type and the neutrophil/ lymphocyte ratio as a biomarker. Asian Pac J Cancer Prev, 14, 3159-61.
  7. Liu YC, Zhou SB, Gao F, et al (2013). Chemotherapy and late course three dimensional conformal radiotherapy for treatment of patients with stage III non- small cell lung cancer. Asian Pac J Cancer Prev, 14, 2663-5.
  8. Lu YY, Huang XE, Xu L, et al (2013). Potential predictors of sensitivity to pemetrexed as first-line chemotherapy for patients with advanced non-squamous NSCLCs. Asian Pac J Cancer Prev, 14, 2005-8.
  9. Manni JA, Guilleron C, Araujo HA, et al (1989). Lymphocyte imbalance in autoimmunity. Medicina B Aires, 49, 105-8.
  10. Mattes J, Hulett M, Xie W, et al (2003). Immunotherapy of cytotoxic T cell resist ant tumors by T helper 2 cells: an eot axin and AT dependent process. J Exp Med, 197, 387- 93.
  11. Mitropoulos D, Alamanis C, Deliveliotts C, et al (1995).T- lymphocyt e subgoups in the peripheral blood of patients with renal cell carcinoma. Arta Urol Belg, 63, 21-5.
  12. Nakamura H, Kawasaki N, Hagiwara M, et al (2000). Cellular immunologic parameters related to age, gender, and stage in lung cancer patients. Lung Cancer, 28, 139-45.
  13. Nugroho AE, Hermawan A, Nastiti K, (2012). Immunomodulatory effects of hexane insoluble fraction of Ficus septica Burm. F. in doxorubicin-treated rats. Asian Pac J Cancer Prev, 13, 5785-90.
  14. Parkin DM, Brary FB, Pisani P (2005). Global cancer statistics. Cancer J Clin, 55, 74-108.
  15. Wing K, Lindgren S, Kollberg G, et al (2003). CD4+ T cell activation by myelinoligodendrocyte glycoprot ein is suppressed by adult but not cord blood CD4+ T cells. Eur J Immunol, 33, 579-87.

Cited by

  1. Impact of Allogenic and Autologous Transfusion on Immune Function in Patients with Tumors vol.15, pp.1, 2014,
  2. Lymphocyte Subgroups and their Surface Receptors NKG2D and NKG2A in Patients with Non-small Cell Lung Cancer vol.15, pp.6, 2014,
  3. Adoptive Immunotherapy for Small Cell Lung Cancer by Expanded Activated Autologous Lymphocytes: a Retrospective Clinical Analysis vol.16, pp.4, 2015,
  4. Tumor-derived CD4+CD25+ Tregs Inhibit the Maturation and Antigen-Presenting Function of Dendritic Cells vol.16, pp.7, 2015,
  5. The role of chemotherapy and operation on lymphocytes accumulation in peripheral blood obtained from patients with oral squamous cell carcinoma vol.4, pp.1, 2015,
  6. Expression of TNFR2 by regulatory T cells in peripheral blood is correlated with clinical pathology of lung cancer patients vol.64, pp.11, 2015,
  7. Association of neutrophil-lymphocyte ratio and T lymphocytes with the pathogenesis and progression of HBV-associated primary liver cancer vol.12, pp.2, 2017,
  8. Circulating Tumor Cells Were Associated with the Number of T Lymphocyte Subsets and NK Cells in Peripheral Blood in Advanced Non-Small-Cell Lung Cancer vol.2017, pp.1875-8630, 2017,
  9. Expression of GARP Is Increased in Tumor-Infiltrating Regulatory T Cells and Is Correlated to Clinicopathology of Lung Cancer Patients vol.8, pp.1664-3224, 2017,
  10. Immunomethylomics: A Novel Cancer Risk Prediction Tool vol.15, pp.Supplement_2, 2018,