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Morinda citrifolia (Noni) Alters Oxidative Stress Marker and Antioxidant Activity in Cervical Cancer Cell Lines

  • Gupta, Rakesh Kumar (Department of Biochemistry, All India Institute of Medical Science) ;
  • Singh, Neeta (Department of Biochemistry, All India Institute of Medical Science)
  • Published : 2013.08.30

Abstract

Background: Cervical cancer, the second most common cancer in women, has a high mortality rate. Cisplatin, an antitumor agent, is generally used for its treatment. However, the administration of cisplatin is associated with side effects and intrinsic resistance. Morinda citrifolia (Noni), a natural plant product, has been shown to have antioxidant activities in vitro and in vivo. Materials and Methods: Both HeLa and SiHa cervical cancer cell lines were treated with 10% Noni, 10 mg/dl cisplatin, or a combination of both 10% Noni and 10 mg/dl cisplatin for 24 hours. Post culturing, the cells were pelleted and stored at $-70^{\circ}C$ for malondialdehyde and catalase assays. Results: On treatment with Noni, CP, and their combination, the level of MDA decreased by 0.76 fold, 0.49 fold, and 0.68 fold respectively in HeLa cells; and by 0.93 fold, 0.67 fold, and 0.79 fold respectively in SiHa cells, as compared to their controls; whereas catalase activity increased by 1.61 fold, 0.54 fold, and 2.35 fold, respectively in HeLa cells; and by 0.98 fold, 0.39 fold, and 1.85 fold respectively in SiHa cells. Conclusions: A decrease in level of lipid peroxidation and an increase in catalase activity were observed with Noni by itself and the effect ameliorated changes observed with cisplatin when given in combination.

Keywords

Morinda citrifolia (Noni);cisplatin;malondialdehyde;catalase;HeLa;SiHa

References

  1. Alitheen NB, Manaf AA, Yeap SK, et al (2010). Immunomodulatory effects of damnacanthal isolated from roots of Morinda elliptica. Pharm Biol, 48, 446-52. https://doi.org/10.3109/13880200903168031
  2. Alshatwi AA, Shafi G, Hasan TN, et al (2011). Apoptosis-mediated inhibition of human breast cancer cell proliferation by lemon citrus extract. Asian Pac J Cancer Prev, 12, 1555-9.
  3. Borek C (2004b). Dietary antioxidants and human cancer. Integr Cancer Ther, 3, 333-41. https://doi.org/10.1177/1534735404270578
  4. Bullo M, Lamuela-Raventos R, Salas-Salvado J (2011). Mediterranean diet and oxidation: nuts and olive oil as important sources of fat and antioxidants. Curr Top Med Chem, 11, 1797-810. https://doi.org/10.2174/156802611796235062
  5. Gupta RK, Banerjee A, Pathak S, et al (2013). Induction of mitochondrial-mediated apoptosis by morinda citrifolia (noni) in human cervical cancer cells. Asian Pac J Cancer Prev, 14, 237-42. https://doi.org/10.7314/APJCP.2013.14.1.237
  6. Gupta RK, Patel AK, Kumari R, et al (2012). Interaction between oxidative stress, lipid profile and antioxidants in breast cancer: a case-control study. Asian Pac J Cancer Prev, 13, 6295-8. https://doi.org/10.7314/APJCP.2012.13.12.6295
  7. Jemal A, Bray F, Center MM, et al (2011). Global cancer statistics. CA Cancer J Clin, 61, 69-90. https://doi.org/10.3322/caac.20107
  8. Hernandez-Flores G, Ortiz-Lazareno PC, Lerma-Diaz JM, et al (2011). Pentoxifylline sensitizes human cervical tumor cells to cisplatin-induced apoptosis by suppressing NF-kappa B and decreased cell senescence. BMC Cancer, 11, 483. https://doi.org/10.1186/1471-2407-11-483
  9. Kostova I (2006). Platinum complexes as anticancer agents. recent patents on anti-cancer. Drug Discovery, 1, 1-22.
  10. Ma DL, West BJ, Su CX, et al (2007). Evaluation of the ergogenic potential of noni juice. Phytother Res, 21, 1100-1. https://doi.org/10.1002/ptr.2188
  11. Mohd-Zin Z, Abdul-Hamid A, Osman A (2002). Antioxidative activity of extracts from Mengkudu (Morinda citrifolia L.) root, fruit and leaf. Food Chem, 78, 227-31. https://doi.org/10.1016/S0308-8146(01)00402-2
  12. Pathak S, Bhatla N, Singh N (2012). Cervical cancer pathogenesis is associated with one carbon metabolism. Mol Cell Biochem, 369, 1-7. https://doi.org/10.1007/s11010-012-1362-3
  13. Peerapatdit T, Patchanans N, Likidlilid A, Poldee S, Sriratanasathavorn C (2006). Plasma lipid peroxidation and antioxidiant nutrients in Type 2 Diabetic patients. J Med Assoc Thai, 89, 147-55.
  14. Serafini MR, Santos RC, Guimaraes AG, et al (2011). Morinda citrifolia Linn leaf extract possesses antioxidant activities and reduces nociceptive behavior and leukocyte migration. J Med Food, 14, 1159-66. https://doi.org/10.1089/jmf.2010.0254
  15. Simone CB 2nd, Simone NL, Simone V, Simone CB (2007). Antioxidants and other nutrients do not interfere with chemotherapy or radioation therapy and can increase kill and increase survival. Part 1. Altern Ther Health Med, 13, 22-8.
  16. Su BN, Pawlus AD, Jung HA, et al (2005). Chemical constituents of the fruits of morinda citrifolia (Noni) and their antioxidant activity. J Nat Prod, 68, 592-5. https://doi.org/10.1021/np0495985
  17. Wang MY, Lutfiyya MN, Weidenbacher-Hoper V, et al (2009). Antioxidant activity of Noni juice in heavy smokers. Chem Cent J, 3, 13. https://doi.org/10.1186/1752-153X-3-13
  18. Wong YH, Abdul Kadir H (2012). Induction of mitochondria-mediated apoptosis in ca ski human cervical cancer cells triggered by mollic acid arabinoside isolated from leea indica. Evid Based Complement Alternat Med, 2012, 684740

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