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Curcumin Inhibits Human Non-small Cell Lung Cancer A549 Cell Proliferation Through Regulation of Bcl-2/Bax and Cytochrome C

  • Li, Yue (Department of Medical Oncology, The Third Affiliated Hospital of Harbin Medical University) ;
  • Zhang, Shuai (Department of Medical Oncology, The Third Affiliated Hospital of Harbin Medical University) ;
  • Geng, Jian-Xiong (Department of Medical Oncology, The Third Affiliated Hospital of Harbin Medical University) ;
  • Hu, Xiao-Yang (Staff Room of Prescription, Heilongjiang University of Chinese Medicine)
  • Published : 2013.08.30

Abstract

We intended to study the mechanism of the inhibitory action of curcumin on human non-small cell lung cancer A549 cell. The cell growth was determined by CCK-8 assay, and the results indicated that curcumin inhibited the cell proliferation in a concentration dependent manner. And to further confirm the relative anti-cancer mechanism of curcumin, RT-PCR was carried out to analysis the expression of relative apoptotic proteins Bax, Bcl-2. We found that curcumin could up-regulate the expression of Bax but down-regulate the expression of Bcl-2 in A549 cells. In addition, curcumin affect the mitochondrial apoptosis pathway. These results suggested that curcumin inhibited cancer cell growth through the regulation of Bcl-2/Bax and affect the mitochondrial apoptosis pathway.

Keywords

Curcumin;Bcl-2;Bax;cytochrome C;apoptosis;NSCLC cells

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