DOI QR코드

DOI QR Code

Bcl-2 Gene Expression in Human Breast Cancers in Iran

  • Rostamizadeh, Leila (Biotechnology Research Center, Tabriz University of Medical Sciences) ;
  • Fakhrjou, Ashraf (Department of Pathology, Tabriz University of Medical Sciences) ;
  • Montazeri, Vahid (Department of Thoracic Surgery, Tabriz University of Medical Sciences) ;
  • Estiar, Mehrdad Asghari (Students' Scientific Research Center, Tehran University of Medical Sciences) ;
  • Naghavi-Behzad, Mohammad (Medical Philosophy and History Research Center, Tabriz University of Medical Sciences) ;
  • Hosseini, Somayyeh (Genetics and Genomics Department, UAB) ;
  • Sakhinia, Masoud (Faculty of Medicine, University of Liverpool) ;
  • Sakhinia, Ebrahim (Biotechnology Research Center, Tabriz University of Medical Sciences)
  • Published : 2013.07.30

Abstract

Background: Breast cancer is among the five most common cancers and ranks first among cancers diagnosed in Iranian women. Screening and treatment of this disease with molecular methods, especially regarding high incidences at early age and advanced stage, is essential. Several genes with altered expression have been identified by cDNA microarray studies in breast cancer, with the Bcl-2 gene indicated as a likely candidate. In this study, we studied Bcl-2 gene expression levels in parallel tumor and non-tumor breast tissues. Materials and Methods: Forty samples including 21 tumor, 16 non tumor (marginal) and 3 benign breast tissues which were all pathologically diagnosed, were subjected to RNA extraction and polyA RT-PCR with the expression level of Bcl-2 quantified using real-time PCR. Results: There is higher expression levels of the Bcl-2 gene in tumor samples compared with marginal samples, but not attaining significance(p>0.05). Bcl-2 expression in 14 (66.7%) of the cases of tumor samples and 9 (56.3%) cases of the marginal samples were positive. Comparison of the expression of the Bcl-2 gene in histological grade showed that a high expression of Bcl-2 was associated with a high histological grade (p<0.41). Conclusions: Our data suggests that dysregulated Bcl-2 gene expression is potentially involved in the pathogenesis of breast cancer. Using gene expression analysis may significantly improve our ability for screening cancer patients and will prove a powerful tool in the diagnosis and prognostic evaluation of the disease whilst aiding the cooperative group trials in the Bcl-2 based therapy project.

Keywords

Breast Cancer;Bcl-2;over expression;real-time quantitative PCR

References

  1. Amirnia M, Babaie-Ghazani A, Fakhrjou A, et al (2012). Immunohistochemical study of cyclooxygenase-2 in skin tumors. J Dermatolog Treat, [Epub ahead of print].
  2. Carlquist JF, Anderson JL (2011). Using pharmacogenetics in real time to guide warfarin initiation A clinician update. Circulation, 124, 2554-9. https://doi.org/10.1161/CIRCULATIONAHA.111.019737
  3. Chipuk JE, Moldoveanu T, Llambi F, et al (2010). The Bcl-2 family reunion. Molecular Cell, 37, 299-310. https://doi.org/10.1016/j.molcel.2010.01.025
  4. Cleland MM, Norris KL, Karbowski M, et al (2010). Bcl-2 family interaction with the mitochondrial morphogenesis machinery. Cell Death and Differentiation, 18, 235-47.
  5. Das S, Sen S, Mukherjee A, et al (2012). Risk factors of breast cancer among women in eastern india: a tertiary hospital based case control study. Asian Pac J Cancer Prev, 13, 4979-81. https://doi.org/10.7314/APJCP.2012.13.10.4979
  6. Dawson S, Makretsov N, Blows FM, et al (2010). Bcl-2 in breast cancer: a favourable prognostic marker across molecular subtypes and independent of adjuvant therapy received. Bri J Cancer, 103, 668-75. https://doi.org/10.1038/sj.bjc.6605736
  7. Ferlay J, Shin HR, Bray F, et al (2012). Globocan 2008 v2.0, cancer incidence and mortality worldwide: IARC CancerBase No. 10 [Internet]. Lyon: International Agency for Research on Cancer; 2010. This report provide estimates of the incidence of major type of cancers, at the national level, for 184 countries of the world.
  8. Ghojazadeh M, Azar ZF, Saleh P, et al (2012). Knowledge and attitude of Iranian University students toward human papilloma virus. Asian Pac J Cancer Prev, 13, 6115-9. https://doi.org/10.7314/APJCP.2012.13.12.6115
  9. Ghojazadeh M, Naghavi-Behzad M, Azar ZF, et al (2012). Parental knowledge and attitudes about human papilloma virus in Iran. Asian Pac J Cancer Prev, 13, 6169-73. https://doi.org/10.7314/APJCP.2012.13.12.6169
  10. Ghojazadeh M, Mohammadi M, Azami-Aghdash S, et al (2013). Estimation of Cancer Cases Using Capture-Recapture Method in Northwest Iran. Asian Pac J Cancer Prev, 14, 3237-41. https://doi.org/10.7314/APJCP.2013.14.5.3237
  11. Gyorffy B, Lanczky A, Eklund AC, et al (2010). An online survival analysis tool to rapidly assess the effect of 22,277 genes on breast cancer prognosis using microarray data of 1,809 patients. Breast Cancer Res Tr, 123, 725-31. https://doi.org/10.1007/s10549-009-0674-9
  12. Harirchi I, Kolahdoozan S, Karbakhsh M, et al (2011). Twenty years of breast cancer in Iran: downstaging without a formal screening program. Ann Oncol, 22, 93-7. https://doi.org/10.1093/annonc/mdq303
  13. Hoerger TJ, Ekwueme DU, Miller JW, et al (2011). Estimated effects of the national breast and cervical cancer early detection program on breast cancer mortality. Am J Prev Med, 40, 397-404. https://doi.org/10.1016/j.amepre.2010.12.017
  14. Houssami N, Abraham LA, Miglioretti DL, et al (2011). Accuracy and outcomes of screening mammography in women with a personal history of early-stage breast cancer. J Am Med Assoc, 305, 790-9. https://doi.org/10.1001/jama.2011.188
  15. Jaberipour M, Habibagahi M, Hosseini A, et al (2010). Detection of B cell lymphoma 2, tumor protein 53, and FAS gene transcripts in blood cells of patients with breast cancer. Indian J Cancer, 47, 412-7. https://doi.org/10.4103/0019-509X.73576
  16. Logan J, Edwards K, Saunders NA, et al (2009). Real-time PCR: current technology and applications, Caister Academic Pr. Caister Academic Press: 7-22.
  17. Luo X, Zhang J, Wang H, et al (2012). PolyA RT-PCR-based quantification of microRNA by using universal TaqMan probe. Biotechnol Lett, 34, 627-33. https://doi.org/10.1007/s10529-011-0813-3
  18. Martinou JC, Youle RJ (2011). Mitochondria in apoptosis: Bcl-2 family members and mitochondrial dynamics. Developmental Cell, 21, 92-101. https://doi.org/10.1016/j.devcel.2011.06.017
  19. Moul JW (2012). Angiogenesis, p53, Bcl-2 and Ki-67 in the progression of prostate cancer after radical prostatectomy. Eur Urol, 35, 399-407.
  20. Razavi SE, Aaghajani H, Haghazali M, et al (2009). The most common cancers in Iranian women. Iran J Public Health, 38, 109-12.
  21. Reddy N, Ninan T, Tabar L, et al (2012). The results of a breast cancer screening camp at a district level in Rural India. Asian Pac J Cancer Prev, 13, 6067-72. https://doi.org/10.7314/APJCP.2012.13.12.6067
  22. Roder DM, de Silva P, Zorbas HN, et al (2012). Adherence to recommended treatments for early invasive breast cancer: decisions of women attending surgeons in the breast cancer audit of Australia and New Zealand. Asian Pac J Cancer Prev, 13, 1675-82. https://doi.org/10.7314/APJCP.2012.13.4.1675
  23. Rajput S, Kumar BN, Sarkar S, et al (2013). Targeted apoptotic effects of thymoquinone and tamoxifen on XIAP mediated akt regulation in breast cancer. PloS one, 8, 61342. https://doi.org/10.1371/journal.pone.0061342
  24. Sadjadi A, Nouraie M, Ghorbani A, et al (2009). Epidemiology of breast cancer in the Islamic republic of Iran: first results from a population-based cancer registry. East Mediterr Health J, 15, 1426-31.
  25. Smerage JB, Budd GT, Doyle GV, et al (2013). Monitoring apoptosis and Bcl-2 on circulating tumor cells in patients with metastatic breast cancer. Molecular Oncology, 7, 680-92. https://doi.org/10.1016/j.molonc.2013.02.013
  26. Tang L, Tan YX, Jiang BG, et al (2013). The prognostic significance and therapeutic potential of hedgehog signaling in intrahepatic cholangiocelluar carcinoma. Clin Canc Res, 19, 2014-24. https://doi.org/10.1158/1078-0432.CCR-12-0349
  27. Van Slooten HJ, Clahsen PC, van Dierendonck JH, et al (1996). Expression of Bcl-2 in node-negative breast cancer is associated with various prognostic factors, but does not predict response to one course of perioperative chemotherapy. Bri J Cancer, 74, 78-85. https://doi.org/10.1038/bjc.1996.319
  28. Won KY, Kim GY, Kim YW, et al (2010). Clinicopathologic correlation of beclin-1 and Bcl-2 expression in human breast cancer. Hum Pathol, 41, 107-12. https://doi.org/10.1016/j.humpath.2009.07.006
  29. Yan JJ, Chen FF, Tsai YC, et al (2009). Immunohistochemical detection of Bcl-2 protein in small cell carcinomas. Oncology, 53, 6-11.

Cited by

  1. Knowledge Production Status of Iranian Researchers in the Gastric Cancer Area: Based on the Medline Database vol.15, pp.12, 2014, https://doi.org/10.7314/APJCP.2014.15.12.5083
  2. Low Expression of the bcl2 Gene in Gastric Adenocarcinomas in Mazandaran Province of Iran vol.16, pp.14, 2015, https://doi.org/10.7314/APJCP.2015.16.14.6067
  3. Ki-67/MIB-1 as a Prognostic Marker in Cervical Cancer - a Systematic Review with Meta-Analysis vol.16, pp.16, 2015, https://doi.org/10.7314/APJCP.2015.16.16.6997
  4. Expression of Cox-2 and Bcl-2 in Paget's Disease of the Breast vol.16, pp.3, 2015, https://doi.org/10.7314/APJCP.2015.16.3.1041
  5. Gene Expression Analyses of HER-2/neu and ESR1 in Patients with Breast Cancer vol.60, pp.0, 2017, https://doi.org/10.1590/1678-4324-2017160733