DOI QR코드

DOI QR Code

MicroRNA-16 Inhibits Bladder Cancer Proliferation by Targeting Cyclin D1

  • Jiang, Qi-Quan ;
  • Liu, Bin ;
  • Yuan, Tao
  • Published : 2013.07.30

Abstract

MicroRNA-16 (miR-16) has been demonstrated to regulate proliferation and apoptosis in many types of cancers, but its biological function in bladder cancer remains unknown. Here, we found expression of miR-16 to be downregulated in bladder cancer in comparison with the adjacent normal tissues. Enforced expression of miR-16 was able to inhibit cell proliferation in TCHu-1 cells, in line with results for miR-16 antisense oligonucleotides (antisense miR-16). At the molecular level, our results further revealed that cyclin D1 expression was negatively regulated by miR-16. Therefore, the data reported here demonstrate that miR-16 is an important regulator in bladder cancer, which will contribute to better understanding of important mis-regulated miRNAs.

Keywords

MicroRNA-16;Cyclin D1;bladdercancer;cell proliferation

References

  1. Berezikov E (2011). Evolution of microRNA diversity and regulation in animals. Nat Rev Genet, 12, 846-60. https://doi.org/10.1038/nrg3079
  2. Dip N, Reis ST, Timoszczuk LS, et al (2012). Stage, grade and behavior of bladder urothelial carcinoma defined by the microRNA expression profile. J Urol, 188, 1951-6. https://doi.org/10.1016/j.juro.2012.07.004
  3. Esteller M (2011). Non-coding RNAs in human disease. Nat Rev Genet, 12, 861-74. https://doi.org/10.1038/nrg3074
  4. Hanlon K, Rudin CE, Harries LW (2009). Investigating the targets of MIR-15a and MIR-16-1 in patients with chronic lymphocytic leukemia (CLL). PLoS One, 4, e7169. https://doi.org/10.1371/journal.pone.0007169
  5. Hu Z, Lin Y, Chen H, et al (2013). MicroRNA-101 suppresses motility of bladder cancer cells by targeting c-Met. Biochem Biophys Res Commun, 435, 82-7. https://doi.org/10.1016/j.bbrc.2013.04.042
  6. Jacobs BL, Lee CT, Montie JE (2010). Bladder cancer in 2010: how far have we come? CA Cancer J Clin, 60, 244-72. https://doi.org/10.3322/caac.20077
  7. Linsley PS, Schelter J, Burchard J, et al (2007). Transcripts targeted by the microRNA-16 family cooperatively regulate cell cycle progression. Mol Cell Biol, 27, 2240-52. https://doi.org/10.1128/MCB.02005-06
  8. Ma Q, Wang X, Li Z, et al (2013). microRNA-16 represses colorectal cancer cell growth in vitro by regulating the p53/survivin signaling pathway. Oncol Rep, 29, 1652-8.
  9. Majid S, Dar AA, Saini S, et al (2012). MicroRNA-1280 inhibits invasion and metastasis by targeting ROCK1 in bladder cancer. PLoS One, 7, e46743. https://doi.org/10.1371/journal.pone.0046743
  10. Malmstrom PU, Loskog AS, Lindqvist CA, et al (2010). AdCD40L immunogene therapy for bladder carcinoma the first phase I/IIa trial. Clin Cancer Res, 16, 3279-87. https://doi.org/10.1158/1078-0432.CCR-10-0385
  11. Neely LA, Rieger-Christ KM, Neto BS, et al (2010). A microRNA expression ratio defining the invasive phenotype in bladder tumors. Urol Oncol, 28, 39-48. https://doi.org/10.1016/j.urolonc.2008.06.006
  12. Noguchi S, Yasui Y, Iwasaki J, et al (2013) Replacement treatment with microRNA-143 and -145 induces synergistic inhibition of the growth of human bladder cancer cells by regulating PI3K/Akt and MAPK signaling pathways. Cancer Lett, 328, 353-61. https://doi.org/10.1016/j.canlet.2012.10.017
  13. O'Kelly F, Marignol L, Meunier A, et al (2012). MicroRNAs as putative mediators of treatment response in prostate cancer. Nat Rev Urol, 9, 397-407. https://doi.org/10.1038/nrurol.2012.104
  14. Shimizu T, Suzuki H, Nojima M, et al (2013). Methylation of a Panel of MicroRNA Genes Is a Novel Biomarker for Detection of Bladder Cancer. Eur Urol, 63, 1091-100. https://doi.org/10.1016/j.eururo.2012.11.030
  15. Song T, Zhang X, Zhang L, et al (2013). miR-708 promotes the development of bladder carcinoma via direct repression of Caspase-2. J Cancer Res Clin Oncol, 139, 1189-98. https://doi.org/10.1007/s00432-013-1392-6
  16. Sun CY, She XM, Qin Y, et al (2013). miR-15a and miR-16 affect the angiogenesis of multiple myeloma by targeting VEGF. Carcinogenesis, 34, 426-35. https://doi.org/10.1093/carcin/bgs333
  17. Takeshita F, Patrawala L, Osaki M, et al (2010). Systemic delivery of synthetic microRNA-16 inhibits the growth of metastatic prostate tumors via downregulation of multiple cell-cycle genes. Mol Ther, 18, 181-7. https://doi.org/10.1038/mt.2009.207
  18. van Kouwenhove M, Kedde M, Agami R (2011). MicroRNA regulation by RNA-binding proteins and its implications for cancer. Nat Rev Cancer, 11, 644-56. https://doi.org/10.1038/nrc3107
  19. Vardouli L, Lindqvist C, Vlahou K, et al (2009). Adenovirus delivery of human CD40 ligand gene confers direct therapeutic effects on carcinomas. Cancer Gene Ther, 16, 848-60. https://doi.org/10.1038/cgt.2009.31
  20. Voutsinas GE, Stravopodis DJ (2009). Molecular targeting and gene delivery in bladder cancer therapy. J Buon, 14, S69-78.
  21. Wang S, Xue S, Dai Y, et al (2012). Reduced expression of microRNA-100 confers unfavorable prognosis in patients with bladder cancer. Diagn Pathol, 7, 159. https://doi.org/10.1186/1746-1596-7-159
  22. Zhang X, Wan G, Mlotshwa S, et al (2010). Oncogenic Wip1 phosphatase is inhibited by miR-16 in the DNA damage signaling pathway. Cancer Res, 70, 7176-86. https://doi.org/10.1158/0008-5472.CAN-10-0697
  23. Zhu DX, Miao KR, Fang C, et al (2011). Aberrant microRNA expression in Chinese patients with chronic lymphocytic leukemia. Leuk Res, 35, 730-4. https://doi.org/10.1016/j.leukres.2010.11.005
  24. Pasquinelli AE (2012). MicroRNAs and their targets: recognition, regulation and an emerging reciprocal relationship. Nat Rev Genet, 13, 271-82.

Cited by

  1. Matrine Reduces Proliferation of Human Lung Cancer Cells by Inducing Apoptosis and Changing miRNA Expression Profiles vol.15, pp.5, 2014, https://doi.org/10.7314/APJCP.2014.15.5.2169
  2. Induction of MicroRNA-9 Mediates Cytotoxicity of Curcumin Against SKOV3 Ovarian Cancer Cells vol.15, pp.8, 2014, https://doi.org/10.7314/APJCP.2014.15.8.3363
  3. miR-19a acts as an oncogenic microRNA and is up-regulated in bladder cancer vol.33, pp.1, 2014, https://doi.org/10.1186/s13046-014-0067-8
  4. MicroRNA-137 Upregulation Increases Bladder Cancer Cell Proliferation and Invasion by Targeting PAQR3 vol.9, pp.10, 2014, https://doi.org/10.1371/journal.pone.0109734
  5. Enrichment Analysis Identifies Functional MicroRNA-Disease Associations in Humans vol.10, pp.8, 2015, https://doi.org/10.1371/journal.pone.0136285
  6. MiR-323-5p acts as a Tumor Suppressor by Targeting the Insulin-like Growth Factor 1 Receptor in Human Glioma Cells vol.15, pp.23, 2015, https://doi.org/10.7314/APJCP.2014.15.23.10181
  7. Matrine inhibits the growth and induces apoptosis of osteosarcoma cells in vitro by inactivating the Akt pathway vol.36, pp.3, 2015, https://doi.org/10.1007/s13277-014-2764-5
  8. Regulation of growth of human bladder cancer by miR-192 vol.36, pp.5, 2015, https://doi.org/10.1007/s13277-014-3020-8
  9. MicroRNA-24 upregulation inhibits proliferation, metastasis and induces apoptosis in bladder cancer cells by targeting CARMA3 vol.47, pp.4, 2015, https://doi.org/10.3892/ijo.2015.3117
  10. MiR-16 mediates trastuzumab and lapatinib response in ErbB-2-positive breast and gastric cancer via its novel targets CCNJ and FUBP1 vol.35, pp.48, 2016, https://doi.org/10.1038/onc.2016.151
  11. BCL2 and miR-15/16: from gene discovery to treatment pp.1476-5403, 2017, https://doi.org/10.1038/cdd.2017.159
  12. Artesunate Induces Apoptosis of Bladder Cancer Cells by miR-16 Regulation of COX-2 Expression vol.15, pp.8, 2014, https://doi.org/10.3390/ijms150814298
  13. miRNA-15a/16: as tumor suppressors and more vol.11, pp.16, 2015, https://doi.org/10.2217/fon.15.101
  14. MicroRNA-16 functions as a tumor-suppressor gene in oral squamous cell carcinoma by targeting AKT3 and BCL2L2 vol.233, pp.12, 2018, https://doi.org/10.1002/jcp.26833
  15. Inhibition of miR-1247 on cell proliferation and invasion in bladder cancer through its downstream target of RAB36 vol.43, pp.2, 2018, https://doi.org/10.1007/s12038-018-9755-4