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Hsa-miR-181a-5p Expression and Effects on Cell Proliferation in Gastric Cancer

  • Chen, Gang (Department of Oncology, the Affiliated Jiangning Hospital of Nanjing Medical University) ;
  • Shen, Zhi-Li (Department of Oncology, the Affiliated Jiangning Hospital of Nanjing Medical University) ;
  • Wang, Ling (Key Laboratory of Developmental Genes and Human Diseases, Ministry of Education, Southeast University) ;
  • Lv, Chun-Ye (Department of Oncology, the Affiliated Jiangning Hospital of Nanjing Medical University) ;
  • Huang, Xin-En (Department of Chemotherapy, the Affiliated Jiangsu Cancer Hospital of Nanjing Medical University & Jiangsu Institute of Cancer Research) ;
  • Zhou, Rong-Ping (Department of Oncology, the Affiliated Jiangning Hospital of Nanjing Medical University)
  • Published : 2013.06.30

Abstract

Purpose: MicroRNAs (miRNAs) are small endogenous, non-coding, single-stranded RNAs (approximately 22 nt). Accumulating evidence has shown that aberrant miRNA expression is pronounced and correlated with gastric cancer genesis and progression. Materials and Methods: Expression levels of miR-181a-5p in GC tissues and cell lines were assessed by qRT-PCR and tested for correlation with clinical features. In addition, effects of miR-181a-5p on GC cell growth were investigated. Results: Our findings indicate that miR-181a-5p is upregulated in GC, in correlation with lymph node invasion, nerve invasion and vascular invasion (P<0.05). Enforced expression of miR-181a -5p promoted cell proliferation ability. Conclusions: This study suggested that increased miR-181a-5p is related to GC progression. MiR-181a-5p may represent a potential therapeutic target for GC.

Keywords

miR-181a-5p;GC;qPCR;cell proliferation

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