DOI QR코드

DOI QR Code

Potential Therapeutic Efficacy of Curcumin in Liver Cancer

  • Dai, Xin-Zheng (Liver Transplantation Center, First Affiliated Hospital of Nanjing Medical University, Key Laboratory of Living Donor Liver Transplantation, Ministry of Public Health) ;
  • Yin, Hai-Tao (Department of Radiotherapy, the Central Hospital of Xuzhou, Affiliated Hospital of Southeast University) ;
  • Sun, Ling-Fei (Department of Radiotherapy, the Central Hospital of Xuzhou, Affiliated Hospital of Southeast University) ;
  • Hu, Xiang (Department of Radiotherapy, the Central Hospital of Xuzhou, Affiliated Hospital of Southeast University) ;
  • Zhou, Chong (Department of Radiotherapy, the Central Hospital of Xuzhou, Affiliated Hospital of Southeast University) ;
  • Zhou, Yun (Department of Radiotherapy, the Central Hospital of Xuzhou, Affiliated Hospital of Southeast University) ;
  • Zhang, Wei (Department of Radiotherapy, the Central Hospital of Xuzhou, Affiliated Hospital of Southeast University) ;
  • Huang, Xin-En (Department of Chemotherapy, the Affiliated Jiangsu Cancer Hospital of Nanjing Medical University & Jiangsu Institute of Cancer Research) ;
  • Li, Xiang-Cheng (Liver Transplantation Center, First Affiliated Hospital of Nanjing Medical University, Key Laboratory of Living Donor Liver Transplantation, Ministry of Public Health)
  • Published : 2013.06.30

Abstract

Purpose: Liver cancer, one of the most common cancers in China, is reported to feature relatively high morbidity and mortality. Curcumin (Cum) is considered as a drug possessing anti-angiogenic, anti-inflammation and anti-oxidation effect. Previous research has demonstrated antitumor effects in a series of cancers. Materials and Methods: In this study the in vitro cytotoxicity of Cum was measured by MTT assay and pro-apoptotic effects were assessed by DAPI staining and measurement of caspase-3 activity. In vivo anti-hepatoma efficacy of Cum was assessed with HepG2 xenografts. Results: It is found that Cum dose-dependently inhibited cell growth in HepG2 cells with activation of apoptosis. Moreover, Cum delayed the growth of liver cancer in a dose-dependent manner in nude mice. Conclusions: Cum might be a promising phytomedicine in cancer therapy and further efforts are needed to explore this therapeutic strategy.

Keywords

Curcumin;apoptosis;liver cancer;in vitro;in vivo

References

  1. Bayet-Robert M, Morvan D (2013). Metabolomics reveals metabolic targets and biphasic responses in breast cancer cells treated by curcumin alone and in association with docetaxel. PLoS One, 8, e57971. https://doi.org/10.1371/journal.pone.0057971
  2. Deng QQ, Huang XE, Ye LH, et al (2013). Phase II trial of Loubo(R) (Lobaplatin) and pemetrexed for patients with metastatic breast cancer not responding to anthracycline or taxanes. Asian Pac J Cancer Prev, 14, 413-7. https://doi.org/10.7314/APJCP.2013.14.1.413
  3. Di Carlo I, Toro A(2013). Future challenges for the treatment of liver tumors. Future Oncol, 9, 481-3. https://doi.org/10.2217/fon.13.20
  4. Du Q, Hu B, An HM, et al (2013). Synergistic anticancer effects of curcumin and resveratrol in Hepa1-6 hepatocellular carcinoma cells. Oncol Rep, 29, 1851-8.
  5. El-Azab M, Hishe H, Moustafa Y, et al (2011). Anti-angiogenic effect of resveratrol or curcumin in Ehrlich ascites carcinoma-bearing mice. Eur J Pharmacol, 10, 7-14.
  6. Gao LL, Huang XE, Zhang Q, et al (2011). A Cisplatin and vinorelbine (NP) regimen as a postoperative adjuvant chemotherapy for completely resected breast cancers in China: final results of a phase II clinical trial. Asian Pac J Cancer Prev, 12, 77-80.
  7. Gong P, Huang XE, Chen CY, et al (2012). Comparison on complications of peripherally inserted central catheters by ultrasound guide or conventional method in cancer patients. Asian Pac J Cancer Prev, 13, 1873-5. https://doi.org/10.7314/APJCP.2012.13.5.1873
  8. Gu M, Li SY, Huang XE, et al (2013). A phase II study on continuous infusional paclitaxel and 5-Fu as first-line chemotherapy for patients with advanced esophageal cancer. Asian Pac J Cancer Prev, 13, 5587-91. https://doi.org/10.7314/APJCP.2012.13.11.5587
  9. Huang XE, Li CG, Li Y, et al (2011). Weekly TP regimen as a postoperative adjuvant chemotherapy for completely resected breast cancer in China: final result of a phase II trial. Asian Pac J Cancer Prev, 12, 2797-800.
  10. Huang XE, Wei GL, Huo JG, et al (2013). Intrapleural or intraperitoneal lobaplatin for treatment of patients with malignant pleural effusion or ascites. Asian Pac J Cancer Prev, 14, 2611-4. https://doi.org/10.7314/APJCP.2013.14.4.2611
  11. Jiang Y, Huang XE, Yan PW, et al (2010). Validation of treatment efficacy of a computer-assisted program for breast cancer patients receiving postoperative adjuvant chemotherapy. Asian Pac J Cancer Prev, 11, 1059-62.
  12. Kudo M (2012). Targeted therapy for liver cancer: updated review in 2012. Curr Cancer Drug Targets, 12, 1062-72.
  13. Kumaravel M, Sankar P, Latha P, et al (2013). Antiproliferative effects of an analog of curcumin in Hep-2 cells: a comparative study with curcumin. Nat Prod Commun, 8, 183-6.
  14. Kwon YK, Jun JM, Shin SW, et al (2005). Curcumin decreases cell proliferation rates through BTG2-mediated cyclin D1 down-regulation in U937 cells. Int J Oncol, 26, 1597-603.
  15. Li CG, Huang XE, Li Y, et al (2011). Clinical observations on safety and efficacy of OxyContin(R) administered by rectal route in treating cancer related pain. Asian Pac J Cancer Prev, 12, 2477-8.
  16. Li CG, Huang XE, Li Y, et al (2011). Phase II trial of irinotecan plus nedaplatin (INP) in treating patients with extensive stage small cell lung cancer. Asian Pac J Cancer Prev, 12, 487-90.
  17. Li CG, Huang XE, Xu L, et al (2012). Clinical application of serum tumor associated material (TAM) from non-small cell lung cancer patients. Asian Pac J Cancer Prev, 13, 301-4. https://doi.org/10.7314/APJCP.2012.13.1.301
  18. Li X, Lu X, Xu H, et al (2012). Paclitaxel/tetrandrine coloaded nanoparticles effectively promote the apoptosis of gastric cancer cells based on “oxidation therapy”. Mol Pharm, 9, 222-9. https://doi.org/10.1021/mp2002736
  19. Li X, Xu H, Dai X, et al (2012). Enhanced in vitro and in vivo therapeutic efficacy of codrug-loaded nanoparticles against liver cancer. Int J Nanomedicine, 7, 5183-90.
  20. Li Y, Yan PW, Huang XE, et al (2011). MDR1 gene C3435T polymorphism is associated with clinical outcomes in gastric cancer patients treated with postoperative adjuvant chemotherapy. Asian Pac J Cancer Prev, 12, 2405-9.
  21. Link A, Balaguer F, Shen Y, et al (2013). Curcumin modulates DNA methylation in colorectal cancer cells. PLoS One, 8, e57709. https://doi.org/10.1371/journal.pone.0057709
  22. Liu J, Huang XE, Tian GY, et al (2013). Phase II Study on Safety and Efficacy of Yadanzi(R) (Javanica oil emulsion injection) Combined with Chemotherapy for Patients with Gastric Cancer. Asian Pac J Cancer Prev, 14, 2009-12. https://doi.org/10.7314/APJCP.2013.14.3.2009
  23. Liu W, Li SY, Huang XE, et al (2012). Inhibition of tumor growth in vitro by a combination of extracts from rosa roxburghii tratt and fagopyrum cymosum. Asian Pac J Cancer Prev, 13, 2409-14. https://doi.org/10.7314/APJCP.2012.13.5.2409
  24. Liu YC, Zhou SB, Gao F, et al (2013). Chemotherapy and late course three dimensional conformal radiotherapy for treatment of patients with stage III non- small cell lung cancer. Asian Pac J Cancer Prev, 14, 2663-5. https://doi.org/10.7314/APJCP.2013.14.4.2663
  25. Lu YY, Huang XE, Xu L, et al (2013). Potential predictors of sensitivity to pemetrexed as first-line chemotherapy for patients with advanced non-squamous NSCLCs. Asian Pac J Cancer Prev, 14, 2005-8. https://doi.org/10.7314/APJCP.2013.14.3.2005
  26. Masuelli L, Benvenuto M, Fantini M, et al (2013). Curcumin induces apoptosis in breast cancer cell lines and delays the growth of mammary tumors in neu transgenic mice. J Biol Regul Homeost Agents, 27, 105-19.
  27. Ni CY, Yang Y, Chang YQ, et al (2013). Fast-track surgery improves postoperative recovery in patients undergoing partial hepatectomy for primary liver cancer: A prospective randomized controlled trial. Eur J Surg Oncol, 39, 542-7. https://doi.org/10.1016/j.ejso.2013.03.013
  28. Ono M, Higuchi T, Takeshima M, et al (2013). Antiproliferative and apoptosis-inducing activity of curcumin against human gallbladder adenocarcinoma cells. Anticancer Res, 33, 1861-6.
  29. Shu J, Li CG, Liu YC, et al (2012). Comparison of serum tumor associated material (TAM) with conventional biomarkers in cancer patients. Asian Pac J Cancer Prev, 13, 2399-403. https://doi.org/10.7314/APJCP.2012.13.5.2399
  30. Suckow BK, Suckow MA (2006). Lifespan extension by the antioxidant curcumin in Drosophila melanogaster. Int J Biomed Sci, 2, 402-5.
  31. Sun MQ, Meng AF, Huang XE, et al (2013). Comparison of psychological influence on breast cancer patients between breast-conserving surgery and modified radical mastectomy. Asian Pac J Cancer Prev, 14, 149-52. https://doi.org/10.7314/APJCP.2013.14.1.149
  32. Sun XD, Liu XE, Huang DS (2013). Curcumin reverses the epithelial-mesenchymal transition of pancreatic cancer cells by inhibiting the Hedgehog signaling pathway. Oncol Rep, 29, 2401-7.
  33. Szymczyk KH, Freeman TA, Adams CS, et al (2006). Active caspase-3 is required for osteoclast differentiation. J Cell Physiol, 209, 836-44. https://doi.org/10.1002/jcp.20770
  34. Wei X, Zhou D, Wang H, et al (2013). Effects of pyridine analogs of curcumin on growth, apoptosis and NF-$\kappa{B}$ activity in prostate cancer PC-3 cells. Anticancer Res, 33, 1343-50.
  35. Wu B, Yao H, Wang S, et al (2013). DAPK1 modulates a curcumin-induced G2/M arrest and apoptosis by regulating STAT3, NF-$\kappa{B}$, and caspase-3 activation. Biochem Biophys Res Commun, 434, 75-80. https://doi.org/10.1016/j.bbrc.2013.03.063
  36. Wu XY, Huang XE, You SX, et al (2013). Phase II study of pemetrexed as second or third line combined chemotherapy in patients with colorectal cancer. Asian Pac J Cancer Prev, 14, 2019-22. https://doi.org/10.7314/APJCP.2013.14.3.2019
  37. Xu HX, Huang XE, Li Y, et al (2011). A clinical study on safety and efficacy of Aidi injection combined with chemotherapy. Asian Pac J Cancer Prev, 12, 2233-6.
  38. Xu HX, Huang XE, Qian ZY, et al (2011). Clinical observation of Endostar(R) combined with chemotherapy in advanced colorectal cancer patients. Asian Pac J Cancer Prev, 12, 3087-90.
  39. Xu JW, Li CG, Huang XE, et al (2011). Ubenimex capsule improves general performance and chemotherapy related toxicity in advanced gastric cancer cases. Asian Pac J Cancer Prev, 12, 985-7.
  40. Xu T, Xu ZC, Zou Q, Yu B, Huang XE (2012). P53 Arg72Pro polymorphism and bladder cancer risk--meta-analysis evidence for a link in Asians but not Caucasians. Asian Pac J Cancer Prev, 13, 2349-54. https://doi.org/10.7314/APJCP.2012.13.5.2349
  41. Xu X, Wang L, Xu HQ, et al (2013). Clinical comparison between Paclitaxel Liposome (Lipusu(R)) and Paclitaxel for treatment of patients with metastatic gastric cancer. Asian Pac J Cancer Prev, 14, 2591-4. https://doi.org/10.7314/APJCP.2013.14.4.2591
  42. Yallapu MM, Ebeling MC, Jaggi M, et al (2013). Plasma proteins interaction with curcumin nanoparticles: implications in cancer therapeutics. Curr Drug Metab, 14, 504-15. https://doi.org/10.2174/1389200211314040012
  43. Yan PW, Huang XE, Yan F, et al (2011). Influence of MDR1 gene codon 3435 polymorphisms on outcome of platinum-based chemotherapy for advanced non small cell lung cancer. Asian Pac J Cancer Prev, 12, 2291-4.
  44. Yin H, Guo R, Xu Y, et al (2012). Synergistic antitumor efficiency of docetaxel and curcumin against lung cancer. Acta Biochim Biophys Sin, 44, 147-53. https://doi.org/10.1093/abbs/gmr106
  45. Yin HT, Tian QZ, Guan L (2013). In vitro and in vivo evaluation of the antitumor efficiency of resveratrol against lung cancer. Asian Pac J Cancer Prev, 14, 1703-6. https://doi.org/10.7314/APJCP.2013.14.3.1703
  46. Yin HT, Zhang DG, Wu XL (2013). In vivo evaluation of curcumin-loaded nanoparticles in a A549 xenograft mice model. Asian Pac J Cancer Prev, 14, 409-12. https://doi.org/10.7314/APJCP.2013.14.1.409
  47. Yin HT, Zhang DG, Wu XL, et al (2013). In vivo evaluation of curcumin-loaded nanoparticles in a A549 xenograft mice model. Asian Pac J Cancer Prev, 14, 409-12. https://doi.org/10.7314/APJCP.2013.14.1.409
  48. Yu DS, Huang XE, Zhou JN, et al (2012). A Comparative study on the value of anal preserving surgery for aged people with low rectal carcinoma in Jiangsu, China. Asian Pac J Cancer Prev, 13, 2339-40. https://doi.org/10.7314/APJCP.2012.13.5.2339
  49. Zhan YP, Huang XE, Cao J (2012). Clinical safety and efficacy of Kanglaite(R) (Coix Seed Oil) injection combined with chemotherapy in treating patients with gastric cancer. Asian Pac J Cancer Prev, 13, 5319-21. https://doi.org/10.7314/APJCP.2012.13.10.5319
  50. Zhan YP, Huang XE, Cao J (2012). Clinical study on safety and efficacy of Qinin(R) (cantharidin sodium) injection combined with chemotherapy in treating patients with gastric cancer. Asian Pac J Cancer Prev, 13, 4773-6. https://doi.org/10.7314/APJCP.2012.13.9.4773
  51. Zhang CY, Zhang L, Yu HX, et al (2013). Curcumin inhibits invasion and metastasis in K1 papillary thyroid cancer cells. Food Chem, 139, 1021-8. https://doi.org/10.1016/j.foodchem.2013.02.016
  52. Zhang LQ, Huang XE, Wang J (2011). The cyclin D1 G870A polymorphism and colorectal cancer susceptibility: a metaanalysis of 20 populations. Asian Pac J Cancer Prev, 12, 81-5.
  53. Zhang XZ, Huang XE, Xu YL, et al (2012). Phase II study on voriconazole for treatment of Chinese patients with malignant hematological disorders and invasive aspergillosis. Asian Pac J Cancer Prev, 13, 2415-8. https://doi.org/10.7314/APJCP.2012.13.5.2415
  54. Zhou JN, Huang XE, Ye Z, et al (2009). Weekly paclitaxel/Docetaxel combined with a paltinum in the treatment of advanced non-samll cell lung cancer: a study on efficacy, safety and pre-medication. Asian Pac J Cancer Prev, 10, 1147-50.

Cited by

  1. Co-treatment of THP-1 cells with naringenin and curcumin induces cell cycle arrest and apoptosis via numerous pathways vol.12, pp.6, 2012, https://doi.org/10.3892/mmr.2015.4480
  2. Role of MYH Polymorphisms in Sporadic Colorectal Cancer in China: A Case-control, Population-based Study vol.14, pp.11, 2013, https://doi.org/10.7314/APJCP.2013.14.11.6403
  3. Risk Assessment on Anastomotic Leakage after Rectal Cancer Surgery: An Analysis of 753 Patients vol.14, pp.7, 2013, https://doi.org/10.7314/APJCP.2013.14.7.4447
  4. Acidic Pelvic Drainage as a Predictive Factor For Anastomotic Leakage after Surgery for Patients with Rectal Cancer vol.14, pp.9, 2013, https://doi.org/10.7314/APJCP.2013.14.9.5441
  5. Curcumin Analogue A501 induces G2/M Arrest and Apoptosis in Non-small Cell Lung Cancer Cells vol.15, pp.16, 2014, https://doi.org/10.7314/APJCP.2014.15.16.6893
  6. Reversal of Resistance towards Cisplatin by Curcumin in Cervical Cancer Cells vol.15, pp.3, 2014, https://doi.org/10.7314/APJCP.2014.15.3.1403
  7. Preventive Effect of Hydrazinocurcumin on Carcinogenesis of Diethylnitrosamine-induced Hepatocarcinoma in Male SD Rats vol.15, pp.5, 2014, https://doi.org/10.7314/APJCP.2014.15.5.2115
  8. Curcumin Inhibits MHCC97H Liver Cancer Cells by Activating ROS/TLR-4/Caspase Signaling Pathway vol.15, pp.5, 2014, https://doi.org/10.7314/APJCP.2014.15.5.2329
  9. Anti-Proliferation Effects and Molecular Mechanisms of Action of Tetramethypyrazine on Human SGC-7901 Gastric Carcinoma Cells vol.15, pp.8, 2014, https://doi.org/10.7314/APJCP.2014.15.8.3581
  10. Targeting Cancer with Nano-Bullets: Curcumin, EGCG, Resveratrol and Quercetin on Flying Carpets vol.15, pp.9, 2014, https://doi.org/10.7314/APJCP.2014.15.9.3865
  11. Expression of Epidermal Growth Factor-like Domain 7 is Increased by Transcatheter Arterial Embolization of Liver Tumors vol.16, pp.3, 2015, https://doi.org/10.7314/APJCP.2015.16.3.1191
  12. Inhibitory Effect of Curcumin on Invasion of Skin Squamous Cell Carcinoma A431 Cells vol.16, pp.7, 2015, https://doi.org/10.7314/APJCP.2015.16.7.2813
  13. Application of Joint Detection of AFP, CA19-9, CA125 and CEA in Identification and Diagnosis of Cholangiocarcinoma vol.16, pp.8, 2015, https://doi.org/10.7314/APJCP.2015.16.8.3451
  14. Analysis on Postoperative Efficacy of Radical Hepatectomy for Patients with Non-HBV/HCV Hepatocellular Carcinoma vol.16, pp.8, 2015, https://doi.org/10.7314/APJCP.2015.16.8.3479
  15. Caspase-9 as a therapeutic target for treating cancer vol.19, pp.1, 2015, https://doi.org/10.1517/14728222.2014.961425
  16. Curcumin ameliorate DENA-induced HCC via modulating TGF-β, AKT, and caspase-3 expression in experimental rat model vol.36, pp.3, 2015, https://doi.org/10.1007/s13277-014-2778-z
  17. Spices for Prevention and Treatment of Cancers vol.8, pp.8, 2016, https://doi.org/10.3390/nu8080495
  18. A novel curcumin analogue is a potent chemotherapy candidate for human hepatocellular carcinoma vol.12, pp.5, 2016, https://doi.org/10.3892/ol.2016.5126