- Volume 14 Issue 6
DOI QR Code
Association of XRCC1 Gene Polymorphisms with Breast Cancer Susceptibility in Saudi Patients
- Al Mutairi, Fatima Masoud (Genome Research Chair, Department of Biochemistry, College of Science, King Saud University) ;
- Alanazi, Mohammed (Genome Research Chair, Department of Biochemistry, College of Science, King Saud University) ;
- Shalaby, Manal (Genome Research Chair, Department of Biochemistry, College of Science, King Saud University) ;
- Alabdulkarim, Huda A. (The Comprehensive Cancer Center at King Fahad Medical City) ;
- Pathan, Akbar Ali Khan (Genome Research Chair, Department of Biochemistry, College of Science, King Saud University) ;
- Parine, Narasimha Reddy (Genome Research Chair, Department of Biochemistry, College of Science, King Saud University)
- Published : 2013.06.30
Background: X-ray repair cross-complementing group 1 (XRCC1) plays a key role in the base excision repair pathway, as a scaffold protein that brings together proteins of the DNA repair complex. XRCC1 is reported to be a candidate influence on cancer risk. The aim of our present study was to assess the association of rs1799782 (Arg194Trp) and rs25487 (Arg399Gln) XRCC1 gene polymorphisms with breast cancer in the Saudi population. Materials and Methods: The two SNP's were analyzed in breast cancer patients and healthy control subjects. Genotypes were determined by TaqMan SNP genotype analysis technique and data were analyzed using Chi-square or t test and logistic regression analysis by SPSS16.0 software. Results and Conclusions: Results showed that rs1799782 significantly increased susceptibility to breast cancer with Arg/Trp, Arg/Trp+Trp/Trp genotypes and at Trp allele overall study. It also increased risk of breast cancer in older age patients (above 48) and with the ER positive category. XRCC1rs25487 (Arg399Gln) did not showed any significant association. In conclusion the XRCC1rs1799782 polymorphism may be involved in the etiology of breast cancer in the Saudi population. Confirmation of our findings in larger populations of different ethnicities is warranted.
XRCC1;Saudi population;breast cancer;genetic polymorphism
- Alanazi MS, Pathan AAK, Ajaj SA, et al (2013). DNA Repair Genes XRCC1, XRCC3, XPD, and OGG1 loci polymorphisms among the central region population of Saudi Arabia. Biol Res, (Epub on press).
- Alsbeih GA, El-Sebaie MM, Al-Rajhi NM, et al (2008) Association between XRCC1 G399A polymorphism and late complications to radiotherapy in Saudi head and neck cancer patients. J Egypt Natl Canc Inst, 20, 302-28.
- Breast Cancer Association Consortium (2010). Commonly studied single-nucleotide polymorphisms and breast cancer: results from the breast cancer association consortium. J Natl Cancer Inst, 98, 1382-96.
- Caldecott KW, Aoufouchi S, Johnson P, Shall S (1996). XRCC1 polypeptide interacts with DNA polymerase beta and possibly poly ADP-ribose polymerase, and DNA ligase III is a novel molecular 'nick-sensor' in vitro. Nucleic Acids Res, 24, 4387-94. https://doi.org/10.1093/nar/24.22.4387
- Chacko P, Rajan B, Joseph T, et al (2005). Polymorphisms in DNA repair gene XRCC1 and increased genetic susceptibility to breast cancer. Breast Cancer Res Treat, 89, 15-21. https://doi.org/10.1007/s10549-004-1004-x
- Duell EJ, Millikan RC, Pittman GS, et al (2001). Polymorphisms in the DNA repair gene XRCC1 and breast cancer. Cancer Epidemiol Biomarkers Prev, 10, 217-22.
- Dufloth RM, Arruda A, Heinrich JK, et al (2008). The investigation of DNA repair polymorphisms with histopathological characteristics and hormone receptors in a group of Brazilian women with breast cancer. Genet Mol Res, 7, 574-82. https://doi.org/10.4238/vol7-3gmr376
- Figueiredo JC, Knight JA, Briollais L, et al (2004). Polymorphisms XRCC1-R399Q and XRCC3-T241M and the risk of breast cancer at the ontario site of the breast cancer family registry. Cancer Epidemiol Biomarkers Prev, 13, 583-91.
- Forsti A, Angelini S, Festa F, et al (2004). Single nucleotide polymorphisms in breast cancer. Oncol Rep, 11, 917-22.
- Hu Z, Ma H, Chen F, Wei Q, Shen H (2001). XRCC1 polymorphisms and cancer risk: a meta-analysis of 38 case-control studies. Cancer Epidemiol Biomarkers Prev, 14, 1810-8.
- Hung RJ, Hall J, Bernnan P, Boffestta P (2005). Genetic polymorphisms in the base excision repair pathway and cancer risk: a HuGE review. Am J Epidemiol, 162, 925-42. https://doi.org/10.1093/aje/kwi318
- Jorgensen TJ, Helzlsouer KJ, Clipp SC, et al (2009). DNA repair gene variants associated with benign breast disease in high cancer risk women. Cancer Epidemiol Biomarkers Prev, 18, 346-50. https://doi.org/10.1158/1055-9965.EPI-08-0659
- Kelsey KT, Park S, Nelson HH, Karagas MR (2004). A population-based case-control study of the XRCC1 Arg399Gln polymorphism and susceptibility to bladder cancer. Cancer Epidem Biomar, 13, 1337-41.
- Kim SU, Park SK, Yoo KY, et al (2002). XRCC1 genetic polymorphism and breast cancer risk. Pharmacogenetics, 12, 335-8. https://doi.org/10.1097/00008571-200206000-00010
- Livak KJ (1999). Allelic discrimination using fluorogenic probes and the 5' nuclease assay. Genet Anal-Biomol E, 14, 143-9. https://doi.org/10.1016/S1050-3862(98)00019-9
- Lopez-Cima MF, Gonzalez-Arriaga P, Garcia-Castro L, et al (2007). Polymorphisms in XPC, XPD, XRCC1, and XRCC3 DNA repair genes and lung cancer risk in a population of northern Spain. BMC cancer, 7, 162. https://doi.org/10.1186/1471-2407-7-162
- Moullan N, Cox DG, Angele S, et al (2003). Polymorphisms in the DNA repair gene XRCC1, breast cancer risk, and response to radiotherapy. Cancer Epidemiol Biomarkers Prev, 12, 1168-74.
- Pramanik S, Devi S, Chowdhary S, et al (2011). DNA repair gene polymorphisms at XRCC1, XRCC3, XPD, and OGG1 loci in Maharashtrian population of central India. Chemosphere, 82, 941-6. https://doi.org/10.1016/j.chemosphere.2010.10.100
- Przybylowska-Sygut K, Stanczyk M, Kusinska R, et al (2013). Association of the Arg194Trp and the Arg399Gln polymorphisms of the XRCC1 gene with risk occurrence and the response to adjuvant therapy among Polish women with breast cancer. Clin Breast Cancer, 13, 61-8. https://doi.org/10.1016/j.clbc.2012.09.019
- Ratnasinghe D, Yao SX, Tangrea JA, et al (2001). Polymorphisms of the DNA repair gene XRCC1 and lung cancer risk. Cancer Epidem Biomar, 10, 119-23.
- Saadat, M, Kohan L, Omidvari S (2008). Genetic polymorphisms of XRCC1 (codon 399) and susceptibility to breast cancer in Iranian women, a case-control study. Breast Cancer Res Treat, 111, 549-53. https://doi.org/10.1007/s10549-007-9811-5
- Sambrook J, Fritsch EF, Maniatis T (1989). Molecular Cloning: a laboratory manual. 2nd ed. Cold Spring Harbor Laboratory: Cold Spring Harbor Laboratory Press. pp1659.
- Smith TR, Miller MS, Lohman K, et al (2003). Polymorphisms of XRCC1 and XRCC3 genes and susceptibility to breast cancer. Cancer Lett, 190, 183-90. https://doi.org/10.1016/S0304-3835(02)00595-5
- Sterpone S, Mastellone V, Padua L, et al (2010). Singlenucleotide polymorphisms in BER and HRR genes, XRCC1 haplotypes and breast cancer risk in Caucasian women. J Cancer Res Clin Oncol, 136, 631-6. https://doi.org/10.1007/s00432-010-0791-1
- Sturgis EM, Castillo EJ, Li L, et al (1999). Polymorphisms of DNA repair gene XRCC1 in squamous cell carcinoma of the head and neck. Carcinogenesis, 20, 2125-9. https://doi.org/10.1093/carcin/20.11.2125
- Thompson LH, West MG (2000). XRCC1 keeps DNA from getting stranded. Mutat Res, 459, 1-18. https://doi.org/10.1016/S0921-8777(99)00058-0
- Zhai X, Liu J, Hu Z, et al (2006). Polymorphisms of ADPRT Val762Ala and XRCC1 Arg399Glu and risk of breast cancer in Chinese women: a case control analysis. Oncol Rep, 15, 247-52.
- Breast Cancer in Iraq, Incidence Trends from 2000-2009 vol.15, pp.1, 2014, https://doi.org/10.7314/APJCP.2014.15.1.281
- Interaction of XRCC1 and XPD Gene Polymorphisms with Lifestyle and Environmental Factors Regarding Susceptibility to Lung Cancer in a High Incidence Population in North East India vol.15, pp.5, 2014, https://doi.org/10.7314/APJCP.2014.15.5.1993
- Single nucleotide polymorphisms (SNPs) of ERCC2, hOGG1, and XRCC1 DNA repair genes and the risk of triple-negative breast cancer in Polish women vol.35, pp.4, 2014, https://doi.org/10.1007/s13277-013-1461-0
- The Relationship between Seven Common Polymorphisms from Five DNA Repair Genes and the Risk for Breast Cancer in Northern Chinese Women vol.9, pp.3, 2014, https://doi.org/10.1371/journal.pone.0092083
- Polymorphisms of the XRCC1 gene and breast cancer risk in the Mexican population vol.14, pp.3, 2015, https://doi.org/10.1007/s10689-015-9787-y
- Differences in Incidence, Mortality and Survival of Breast Cancer by Regions and Countries in Asia and Contributing Factors vol.16, pp.7, 2015, https://doi.org/10.7314/APJCP.2015.16.7.2857
- The hOGG1 Ser326Cys Gene Polymorphism and Breast Cancer Risk in Saudi Population vol.23, pp.3, 2017, https://doi.org/10.1007/s12253-016-0146-6
- Genetic Variations of DNA Repair Genes in Breast Cancer pp.1532-2807, 2017, https://doi.org/10.1007/s12253-017-0322-3
- (Thr241Met) Polymorphisms with the Risk of Breast Cancer: A Case–Control Study in Egypt vol.18, pp.11, 2014, https://doi.org/10.1089/gtmb.2014.0191