Asian Pacific Journal of Cancer Prevention

Asian Pacific Journal of Cancer Prevention (아시아태평양암예방학회)
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Associations Between RASSF1A Promoter Methylation and NSCLC: A Meta-analysis of Published Data

  • Liu, Wen-Jian (Department of Chemotherapy, The Affiliated Tumor Hospital of Guangxi Medical University) ;
  • Tan, Xiao-Hong (Department of Chemotherapy, The Affiliated Tumor Hospital of Guangxi Medical University) ;
  • Guo, Bao-Ping (Department of Chemotherapy, The Affiliated Tumor Hospital of Guangxi Medical University) ;
  • Ke, Qing (Department of Chemotherapy, The Affiliated Tumor Hospital of Guangxi Medical University) ;
  • Sun, Jie (Department of Chemotherapy, The Affiliated Tumor Hospital of Guangxi Medical University) ;
  • Cen, Hong (Department of Chemotherapy, The Affiliated Tumor Hospital of Guangxi Medical University)
  • Published : 2013.06.30

Abstract

Background: RASSF1A has been reported to be a candidate tumor suppressor in non-small cell lung cancer (NSCLC). However, the association between RASSF1A promoter methylation and NSCLC remains unclear, particularly in regarding links to clinicopathologic features. Methods: Eligible studies were identified through searching PubMed, EMBASE, Cochrane Library and China National Knowledge Infrastructure (CNKI) databases. Studies were pooled and odds ratios (ORs) with corresponding confidence intervals (CIs) were calculated. Funnel plots were also performed to evaluate publication bias. Results: Nineteen studies involving 2,063 cases of NSCLC and 1,184 controls were included in this meta-analysis. A significant association was observed between RASSF1A methylation and NSCLC in the complete data set (OR = 19.42, 95% CI: 14.04-26.85, P < 0.001). Pooling the control tissue subgroups (heterogeneous/autologous) gave pooled ORs of 32.4 (95% CI, 12.4-84.5) and 17.7 (95% CI, 12.5-25.0) respectively. Racial subgroup (Caucasian/Asian) analysis gave pooled ORs of 26.6 (95% CI, 10.9-64.9) and 20.9 (95% CI, 14.4-30.4) respectively. The OR for RASSF1A methylation in poorly-differentiated vs. moderately/well-differentiated NSCLC tissues was 1.88 (95% CI, 1.32-2.68, P<0.001), whereas there were no significant differences in RASSF1A methylation in relation to gender, pathology, TNM stage and smoking behavior among NSCLC cases. Conclusion: This meta-analysis suggests a significant association between RASSF1A methylation and NSCLC, confirming the role of RASSF1A as a tumor suppressor gene. Large-scale and well-designed case-control studies are needed to validate the associations identified in the present meta-analysis.

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References

  1. Agathanggelou A, Cooper WN, Latif F (2005). Role of the Rasassociation domain family 1 tumor suppressor gene in human cancers. Cancer Res, 65, 3497-508. https://doi.org/10.1158/0008-5472.CAN-04-4088
  2. Akhavan-Niaki H, Samadani AA (2013). DNA Methylation and Cancer Development: Molecular Mechanism. Cell Biochem Biophys.
  3. Anglim PP, Alonzo TA, Laird-Offringa IA (2008). DNA methylation-based biomarkers for early detection of non-small cell lung cancer: an update. Mol Cancer, 7, 81. https://doi.org/10.1186/1476-4598-7-81
  4. Baylin SB (2005). DNA methylation and gene silencing in cancer. Nat Clin Pract Oncol, 2, S4-11. https://doi.org/10.1038/ncponc0354
  5. Brauch H, Johnson B, Hovis J, et al (1987). Molecular analysis of the short arm of chromosome 3 in small-cell and non-smallcell carcinoma of the lung. N Engl J Med, 317, 1109-13. https://doi.org/10.1056/NEJM198710293171803
  6. Burbee DG, Forgacs E, Zochbauer-Muller S, et al (2001). Epigenetic inactivation of RASSF1A in lung and breast cancers and malignant phenotype suppression. J Natl Cancer Inst, 93, 691-9. https://doi.org/10.1093/jnci/93.9.691
  7. Chan MW, Chan LW, Tang NL, et al (2003). Frequent hypermethylation of promoter region of RASSF1A in tumor tissues and voided urine of urinary bladder cancer patients. Int J Cancer, 104, 611-6. https://doi.org/10.1002/ijc.10971
  8. Chen H, Suzuki M, Nakamura Y, et al (2006). Aberrant methylation of RASGRF2 and RASSF1A in human non-small cell lung cancer. Oncol Rep, 15, 1281-5.
  9. Choi JE, Kim DS, Kim EJ, et al (2008). Aberrant methylation of ADAMTS1 in non-small cell lung cancer. Cancer Genet Cytogenet, 187, 80-4. https://doi.org/10.1016/j.cancergencyto.2008.08.001
  10. Choi N, Son DS, Song I, et al (2005). RASSF1A is not appropriate as an early detection marker or a prognostic marker for nonsmall cell lung cancer. Int J Cancer, 115, 575-81. https://doi.org/10.1002/ijc.20916
  11. Dammann R, Li C, Yoon JH, et al (2000). Epigenetic inactivation of a RAS association domain family protein from the lung tumour suppressor locus 3p21.3. Nat Genet, 25, 315-9. https://doi.org/10.1038/77083
  12. Dammann R, Strunnikova M, Schagdarsurengin U, et al (2005). CpG island methylation and expression of tumour-associated genes in lung carcinoma. Eur J Cancer, 41, 1223-36. https://doi.org/10.1016/j.ejca.2005.02.020
  13. Guo M, House MG, Hooker C, et al (2004). Promoter hypermethylation of resected bronchial margins: a field defect of changes? Clin Cancer Res, 10, 5131-6. https://doi.org/10.1158/1078-0432.CCR-03-0763
  14. Hesson LB, Cooper WN, Latif F (2007). The role of RASSF1A methylation in cancer. Dis Markers, 23, 73-87. https://doi.org/10.1155/2007/291538
  15. Hsu HS, Chen TP, Hung CH, et al (2007). Characterization of a multiple epigenetic marker panel for lung cancer detection and risk assessment in plasma. Cancer, 110, 2019-26. https://doi.org/10.1002/cncr.23001
  16. Ito M, Ito G, Kondo M, et al (2005). Frequent inactivation of RASSF1A, BLU, and SEMA3B on 3p21.3 by promoter hypermethylation and allele loss in non-small cell lung cancer. Cancer Lett, 225, 131-9. https://doi.org/10.1016/j.canlet.2004.10.041
  17. Jemal A, Bray F, Center MM, et al (2011). Global cancer statistics. CA Cancer J Clin, 61, 69-90. https://doi.org/10.3322/caac.20107
  18. Kang CY, Zhou HC, Tang SP, Xiao H (2011). Values of promoter hypermethylation of FHIT, p 16, MGMT and RASSF 1A genes in plasma in the diagnosis of lung cancer. Tumor, 31, 729-34.
  19. Kim DS, Cha SI, Lee JH, et al (2007). Aberrant DNA methylation profiles of non-small cell lung cancers in a Korean population. Lung Cancer, 58, 1-6. https://doi.org/10.1016/j.lungcan.2007.04.008
  20. Kontic M, Stojsic J, Jovanovic D, et al (2012). Aberrant promoter methylation of CDH13 and MGMT genes is associated with clinicopathologic characteristics of primary non-small-cell lung carcinoma. Clin Lung Cancer, 13, 297-303. https://doi.org/10.1016/j.cllc.2011.11.003
  21. Kwong J, Lo KW, To KF, et al (2002). Promoter hypermethylation of multiple genes in nasopharyngeal carcinoma. Clin Cancer Res, 8, 131-7.
  22. Li W, Deng J, Jiang P, et al (2012). Methylation of the RASSF1A and RAR(beta) genes as a candidate biomarker for lung cancer. Exp Ther Med, 3, 1067-71.
  23. Lin Q, Geng J, Ma K, et al (2009). RASSF1A, APC, ESR1, ABCB1 and HOXC9, but not p16INK4A, DAPK1, PTEN and MT1G genes were frequently methylated in the stage I non-small cell lung cancer in China. J Cancer Res Clin Oncol, 135, 1675-84. https://doi.org/10.1007/s00432-009-0614-4
  24. Liu Z, Zhao J, Chen XF, et al (2008). CpG island methylator phenotype involving tumor suppressor genes located on chromosome 3p in non-small cell lung cancer. Lung Cancer, 62, 15-22. https://doi.org/10.1016/j.lungcan.2008.02.005
  25. Peng Z, Shan C, Wang H (2010). [Value of promoter methylation of RASSF1A, p16, and DAPK genes in induced sputum in diagnosing lung cancers]. Zhong Nan Da Xue Xue Bao Yi Xue Ban, 35, 247-53.
  26. Shivakumar L, Minna J, Sakamaki T, Pestell R, White MA (2002). The RASSF1A tumor suppressor blocks cell cycle progression and inhibits cyclin D1 accumulation. Mol Cell Biol, 22, 4309-18. https://doi.org/10.1128/MCB.22.12.4309-4318.2002
  27. Song H, Yi J, Zhang Y, Wang R, Chen L (2011). DNA methylation of tumor suppressor genes located on chromosome 3p in non-small cell lung cancer. Chin J Lung Cancer, 14, 233-8.
  28. To KF, Leung WK, Lee TL, et al (2002). Promoter hypermethylation of tumor-related genes in gastric intestinal metaplasia of patients with and without gastric cancer. Int J Cancer, 102, 623-8. https://doi.org/10.1002/ijc.10783
  29. Toyooka S, Maruyama R, Toyooka KO, et al (2003). Smoke exposure, histologic type and geography-related differences in the methylation profiles of non-small cell lung cancer. Int J Cancer, 103, 153-60. https://doi.org/10.1002/ijc.10787
  30. Wang J, Lee JJ, Wang L, et al (2004). Value of p16INK4a and RASSF1A promoter hypermethylation in prognosis of patients with resectable non-small cell lung cancer. Clin Cancer Res, 10, 6119-25. https://doi.org/10.1158/1078-0432.CCR-04-0652
  31. Wang J, Wang B, Chen X, Bi J (2011). The prognostic value of RASSF1A promoter hypermethylation in non-small cell lung carcinoma: a systematic review and meta-analysis. Carcinogenesis, 32, 411-6. https://doi.org/10.1093/carcin/bgq266
  32. Yanagawa N, Tamura G, Oizumi H, et al (2003). Promoter hypermethylation of tumor suppressor and tumor-related genes in non-small cell lung cancers. Cancer Sci, 94, 589-92. https://doi.org/10.1111/j.1349-7006.2003.tb01487.x
  33. Yanagawa N, Tamura G, Oizumi H, et al (2007). Promoter hypermethylation of RASSF1A and RUNX3 genes as an independent prognostic prediction marker in surgically resected non-small cell lung cancers. Lung Cancer, 58, 131-8. https://doi.org/10.1016/j.lungcan.2007.05.011
  34. Yang ZH, Cai YY, Sun LH (2007). Methylation of antioncogen at 3p in non-small cell lung cancer. Chin J Cancer Prev Treat, 14, 363-5.
  35. Yu MY, Tong JH, Chan PK, et al (2003). Hypermethylation of the tumor suppressor gene RASSFIA and frequent concomitant loss of heterozygosity at 3p21 in cervical cancers. Int J Cancer, 105, 204-9. https://doi.org/10.1002/ijc.11051
  36. Zhang H, Zhang S, Zhang Z, et al (2010). Prognostic value of methylation status of RASSF1A gene as an independent factor of non-small cell lung cancer. Chin J Lung Cancer, 13, 311-6.
  37. Zhang Y, Wang R, Song H, et al (2011). Methylation of multiple genes as a candidate biomarker in non-small cell lung cancer. Cancer Lett, 303, 21-8. https://doi.org/10.1016/j.canlet.2010.12.011

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